Evaluation of Siam Catfish Fry Resistance to Edwardsiella ictaluri and Aeromonas hydrophila Bacterial Infections
DOI:
https://doi.org/10.30605/y0k33r70Keywords:
Siam catfish, Edwardsiella ictaluri, Aeromonas hydrophila, LD50, Challenge testAbstract
Catfish is one of the fishery commodities that has important economic value in the aquaculture industry. However, the success of cultivation is often constrained by bacterial disease attacks that cause decreased productivity, high mortality, and economic losses. One of the main diseases that affect catfish is Enteric Septicemia of Catfish (ESC) and Motile Aeromonads Septicemia (MAS). This activity aims to evaluate the resistance of catfish to the bacteria Edwardsiella ictaluri and Aeromonas hydrophila. The method used was an experiment with the test treatment of the bacteria Edwardsiella ictaluri and Aeromonas hydrophila on selected Siamese catfish and UPR Siamese catfish. Before the challenge test was carried out, an experiment was first carried out to determine the LD50 value in each bacteria. The parameters observed included clinical symptoms and mortality rates during maintenance. The observation data were tabulated using the Microsoft Excel 2017 program tool and analyzed descriptively. The results of the study showed that the LD50 value of catfish infected with Edwardsiella ictaluri bacteria.obtained at a concentration of 1.2x108 CFU/mL, while in Aeromonas hydrophila bacteria at a concentration of 1.8x109 CFU/mL, the selected Siamese catfish had better resistance to the bacterial pairing of Edwardsiella ictaluri and Aeromonas hydrophila than the Siam catfish UPR.
References
Abdelhamed, H., Lawrence, M. L., & Karsi, A. (2017). The role of Edwardsiella ictaluri TonB mutant in Edwardsiella ictaluri virulence. Frontiers in Physiology, 8(DEC), 1–8. https://doi.org/10.3389/fphys.2017.01066
Adeolu, M., Alnajar, S., Naushad, S., & Gupta, R. S. (2016). Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: Proposal for enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. International Journal of Systematic and Evolutionary Microbiology, 66(12), 5575–5599. https://doi.org/10.1099/ijsem.0.001485
Andriyanto S., Tahapari, E dan Insan, I.. 2012. Pendederan Ikan Patin Sebagai Pendukung Program Restocking di Waduk Malahayu–Brebes, Jawa Tengah. Prosiding Indoaqua - Forum Inovasi Teknologi Akuakultur 2012. Jakarta, pp 211 – 215.
Arnold, T., Neubauer, H., Nikolaou, K., Roesler, U., & Hensel, A. (2004). Identification of Yersinia enterocolitica in Minced Meat: A Comparative Analysis of API 20E, Yersinia Identification Kit and a 16S rRNA-based PCR Method. Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, 51(1), 23–27. https://doi.org/10.1046/j.1439-0450.2003.00718.x
Austin B dan Austin DA. 1987. Bacterial Fish Pathogens : Disease in Farmed and Wils Fish. John Willy and Sons Ltd. England
Bakiyev, S., Smekenov, I., Zharkova, I., Kobegenova, S., & Sergaliyev, N. (2022). Isolation , identification , and characterization of pathogenic Aeromonas hydrophila from critically endangered Acipenser baerii. Aquaculture Reports, 26(May), 101293. https://doi.org/10.1016/j.aqrep.2022.101293
Carson, J., Wagner, T., Wilson, T., & Donachie, L. (2001). Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix. Journal of Applied Microbiology, 90(2), 190–200. https://doi.org/10.1046/j.1365-2672.2001.01231.x
Carriero, M.M., Mendes Maia, A.A., Moro Sousa, R.L. and Henrique-Silva, F. (2016), Characterization of a new strain of Aeromonas dhakensis isolated from diseased pacu fish (Piaractus mesopotamicus) in Brazil. J Fish Dis, 39: 1285-1295. https://doi.org/10.1111/jfd.12457
Chen H, Yuan G, Su J, Liu X. 2019. Hematological and immune genes responsses in yellow catfish (Pelteobagrus fulvidraco) with septicemia induced by Edwardsiella ictaluri. J Fish and Shellfish Immunology. https://doi.org/10.1016/j.fsi.2019.11.071
Chowdhury, M. A., & Roy, N. C. (2020). Probiotic supplementation for enhanced growth of striped catfish (Pangasianodon hypophthalmus) in cages. Aquaculture Reports, 18, 100504. https://doi.org/10.1016/j.aqrep.2020.100504
Darmawan, B. D., & Rohaendi, O. E. (2014). Zoonosis : Infeksi penyakit ikan terhadap manusia akibat kesalahan manajemen dan penanganan ikan maupun produk olahannya. Journal of Aquatropica Asia, 1, 2–9.
Dehghani, S., Akhlaghi, M., & Dehghani, M. (2012). Efficacy of formalin-killed, heat-killed and lipopolysaccharide vaccines against motile aeromonads infection in rainbow trout (Oncorhynchus mykiss). Global Veterinaria, 9(4), 409–415. https://doi.org/10.5829/idosi.gv.2012.9.4.6591
Eissa, N., & Wang, H. P. (2016). Transcriptional stress responses to environmental and husbandry stressors in aquaculture species. Reviews in Aquaculture, 8(1), 61–88. https://doi.org/10.1111/raq.12081
Erickson VI, Khoi LM, Hounmanou YMG, Dung TT, Phu MT, Dalsgaard A. 2023. Comparative genomic analysis of Aeromonas dhakensis and Aeromonas hydrophila from diseased striped catfish fingerlings cultured in Vietnam. Frontiers in Microbiology. 14:1254781. doi:10.3389/fmicb.2023.1254781
Fu, G. H., Liu, F., Xia, J. H., & Yue, G. H. (2014). The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia. International Journal of Molecular Sciences, 15(12), 22028–22041. https://doi.org/10.3390/ijms151222028
Gustiano, R. 2009. Pangasiid catfishes of Indonesia. Buletin Plasma Nutfah 15(2): 91-100.
Hawke, J. P., McWhorter, A. C., Steigerwalt, A. G., & Brenner, D. J. (1981). Edwardsiella ictaluri sp. nov., the causative agent of enteric septicemia of catfish. International Journal of Systematic Bacteriology, 31(4), 396–400. https://doi.org/10.1099/00207713-31-4-396
Hawke JP, Kent M, Rogge M, Baumgartner W, Wiles J, Shelley J, Savolainen LC, Wagner R, Murray K, Peterson TS. 2013. Edwardsiellosis Caused by Edwardsiella ictaluri in Laboratory Populations of Zebrafish Danio rerio. Journal of Aquatic Animal Health 25(3): 171-183. https://doi.org/ 10.1018/08997659.2013.782226
Hoa, T. T. T., Boerlage, A. S., Duyen, T. T. M., Thy, D. T. M., Hang, N. T. T., Humphry, R. W., & Phuong, N. T. (2021). Nursing stages of striped catfish (Pangasianodon hypophthalmus) in Vietnam: Pathogens, diseases and husbandry practices. Aquaculture, 533(July 2020), 736114. https://doi.org/10.1016/j.aquaculture.2020.736114
Hussain Bhat, R. A., Thakuria, D., Dubey, M. K., Tandel, R. S., Sharma, P., Khangembam, V. C., Dash, P., Tripathi, G., & Sarma, D. (2021). Lethal dose and histopathological alterations induced by Aeromonas salmonicida in experimentally challenged common carp, Cyprinus carpio. Microbial Pathogenesis, 158(April), 105110. https://doi.org/10.1016/j.micpath.2021.105110
Kartamihardja, E. S., Purnomo, K., Koeshendrajana, S., Umar, C. 2011. Karakteristik limnologis, perkembangan populasi ikan patin siam (Pangasionodon hypophthalmus) introduksi dan perikanan di waduk wonogiri, jawa tengah. Jurnal Kebijakan Perikanan Indonesia 3(1), 37-50
KKP, 2022. Kelautan dan Perikanan Dalam Angka Tahun 2022. Pusat Data, Statistik dan Informasi. Kementerian Kelautan Dan Perikanan. 348 hal.
[KKP] Kementerian Kelautan dan Perikanan. 2024. Laporan Kinerja Kementerian Kelautan dan Perikanan 2023 Edisi ke-1. Jakarta: KKP Pr.
Le, T. S., Nguyen, T. H., Vo, H. P., Doan, V. C., Nguyen, H. L., Tran, M. T., Tran, T. T., Southgate, P. C., & İpek Kurtböke, D. (2018). Protective effects of bacteriophages against Aeromonas hydrophila species causing motile aeromonas Septicemia (MAS) in striped catfish. Antibiotics, 7(1), 1–11. https://doi.org/10.3390/antibiotics7010016
Lukistyowati, I. and Kurniasih, 2012. Pelacakan Gen Aerolysin dari Aeromonas hydrophila pada Ikan Mas yang Diberi Pakan Ekstrak Bawang Putih. Jurnal Veteriner, 13(1), pp.43–50.
Magnadottir, B. (2010). Immunological Control of Fish Diseases. Mar Biotechnol 12, 361–379 https://doi.org/10.1007/s10126-010-9279-x
Mok, D., Nabulsi, R., & Chowdhury, S. (2018). Conformity evaluation checklists of API 20 E for ISO 15189:2012 internal auditing: An optimisation tool for medical laboratories. New Zealand Journal of Medical Laboratory Science, 72(3), 90–95. https://www.researchgate.net/publication/328876253
Nhinh, D.T., Le, D.V., Van, K.V., Huong Giang, N.T., Dang, L.T., Hoai, T.D., 2021. Prevalence, virulence gene distribution and alarming the multidrug resistance of Aeromonas hydrophila associated with disease outbreaks in freshwater aquaculture. Antibiotics 10 (5), 532. https://doi.org/10.1046/j.1365-2672.2002.01711.x.
Nusbaum KE, Morrison EE. 2002. Edwardsiella ictaluri bacteraemia elicits shedding of Aeromonas hydrophila complex in latently infected channel catfish, Ictalurus punctatus (Rafinesque). J. of Fish Diseases, 25:343-350.
Noga EJ. 2000. Fish Disease : Diagnosis and Treatment. Iowa State Press, A Blackwell Publishing Company, Iowa. 366 hal.
Payne, C. J., Grace, K., Phuong, V. H., Phuoc, N. N., Dung, T. T., Phuoc, L. H., & Crumlish, M. (2023). Exploring the genetic diversity of Edwardsiella ictaluri in Vietnamese striped catfish (Pangasianodon hypophthalmus) farms over a 20-year period. Frontiers in Marine Science, 10(December), 1–13. https://doi.org/10.3389/fmars.2023.1270968
Peterman, M. A., & Posadas, B. C. (2019). Direct Economic Impact of Fish Diseases on the East Mississippi Catfish Industry. North American Journal of Aquaculture, 81(3), 222–229. https://doi.org/10.1002/naaq.10090
Phuoc, N. N., Richards, R., & Crumlish, M. (2020). Environmental conditions influence susceptibility of striped catfish Pangasianodon hypophthalmus (Sauvage) to Edwardsiella ictaluri. Aquaculture, 523(May 2019), 735226. https://doi.org/10.1016/j.aquaculture.2020.735226
Plumb, J. A., & Hanson, L. A. (2011). Health maintenance and principal microbial diseases of cultured fishes (3rd ed.). Wiley-Blackwell.
Pouyaud, L., R. Gustiano and M. Legendre. 1998. Phylogenetic relationship among pangasiid catfish species (Siluriformes, Pangasiidae) and new insights on their zoogeography. In: M. Legendre and A. Parisele (eds.). The Biological Diversity and Aquaculture of Clariid and Pangasiid Catfishes in South-East Asia. Proceeding of The Mid-Term Workshop of the Catfish Asia Project, 11-15 May 1998. Cantho - Vietnam. Pp. 49-56
Purwaningsih U, Novita H, Sugiani D, Andriyanto S. 2019. Identifikasi dan karakterisasi bakteri Edwardsiella ictaluri penyebab penyakit Enteric Septicemia of Catfish (ESC) pada ikan patin (Pangasius sp.). Jurnal Riset Akuakultur 14 (1): 47-57.
Putri, F. A., Rahman, & Arif, M. M. (2022). Determination of LC50 and Clinical Symptoms of Aeromonas hydrophila Infection on the Fingerlings of Semah (Tor soro), the Indonesian Native Freshwater Fish. E3S Web of Conferences, 348. https://doi.org/10.1051/e3sconf/202234800019
Rathod NB, Pagarkar AU, Pujari KH, Shingare PE, Satam SB, Phadke GG, Gaikwad BV. 2018. Status of Valuable Components from Pangasius: A Review. Int.J.Curr.Microbiol.App.Sci 7(4): 2106-2120. https://doi.org/10.20546/ijcmas.2018.704.241
Samayanpaulraj, V., Sivaramapillai, M., Palani, S. N., Govindaraj, K., Velu, V., & Ramesh, U. (2020). Identification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan. Food Science and Nutrition, 8(2), 1272–1283. https://doi.org/10.1002/fsn3.1416
Santos, Y., Romalde, J. L., Bandín, I., Magariños, B., Núñez, S., Barja, J. L., & Toranzo, A. E. (1993). Usefulness of the API-20E system for the identification of bacterial fish pathogens. Aquaculture, 116(2–3), 111–120. https://doi.org/10.1016/0044-8486(93)90002-G
Semwal, A., Kumar, A., & Kumar, N. (2023). Heliyon Review article A review on pathogenicity of Aeromonas hydrophila and their mitigation through medicinal herbs in aquaculture. Heliyon, 9(3), e14088. https://doi.org/10.1016/j.heliyon.2023.e14088
Shoemaker, C., Xu, D. H., LaFrentz, B., & LaPatra, S. (2015). Overview of fish immune system and infectious diseases. Dietary Nutrients, Additives and Fish Health, 1–24. https://doi.org/10.1002/9781119005568.ch1
Song, X., Zhao, J., Bo, Y., Liu, Z., Wu, K., & Gong, C. (2014). Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model. Aquaculture, 434, 171–178. https://doi.org/10.1016/j.aquaculture.2014.08.015
Susandi, F., & Rosmawati, M. (2017). Increased Immunity of Giant Gouramy (Osphronemus gouramy Lac.) Fry Against Aeromonas hydrophila Bacteria Used Roselle (Hibiscus sabdariffa L.). Jurnal Mina Sains, 3(2), 1–12.
Supriyadi, H., Widiyati, A., Sunarto, A., & Prihadi, T.H. 2005. Keragaan penyakit bacterial ikan nila (Oreochromis niloticus) pada keramba jaring apung (KJA) di Lokasi Berbeda, J. Pen. Perik. Indonesia 11(7): 35–41
Susanti, W., Indrawati, A., & Pasaribu, F. H. (2016). Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus. Jurnal Akuakultur Indonesia, 15(2), 99–107. https://doi.org/10.19027/jai.15.99-107
Triet TH, Tinh BT, Hau LV, Huong TV, Binh NQ. 2019. Development and potential use of an Edwardsiella ictaluri wzz mutant as a live attenuated vaccine against enteric septicemia in Pangasius hypophthalmus (Tra catfish). Fish Shellfish Immunol. 87: 87–95. https://doi.org/10.1016/j.fsi.2019.01.005.
Ugarte-Torres, A., Perry, S., Franko, A., & Church, D. L. (2018). Multidrug-resistant Aeromonas hydrophila causing fatal bilateral necrotizing fasciitis in an immunocompromised patient: A case report. Journal of Medical Case Reports, 12(1), 1–7. https://doi.org/10.1186/s13256-018-1854-1
Yuasa K, Kholidin EB, Panigoro N, Hatai K. 2003. First isolation of Edwardsiella ictaluri from cultured striped catfish Pangasius hypophthalmus in Indonesia. Fish Pathol 38 (4): 181-183.
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