ANALISIS FECAL COLIFORM PADA AIR LIMBAH MENGGUNAKAN METODE MOST PROBABLE NUMBER (MPN)

Authors

DOI:

https://doi.org/10.30605/xmcbmm96

Keywords:

Air limbah, fecal coliform, Most Probable Number, Lauryl tryptose broth, EC Broth

Abstract

Domestic wastewater has the potential to pollute the environment because it contains pathogenic microorganisms, including fecal coliform bacteria as indicators of biological contamination. This study aimed to determine the presence of fecal coliform in wastewater samples using the Most Probable Number (MPN) method. The research was conducted from February 2 to February 6, 2026, at the Public Health Laboratory Center (BLKM) Medan. The study used a descriptive method with a five-tube MPN technique through presumptive testing using Lauryl Tryptose Broth (LTB) medium and confirmed testing using EC Broth referring to EPA Method 1680. The results showed variations in fecal coliform MPN values in each wastewater sample. Samples AL8, AL9, AL11, and AL12 showed the highest MPN value of >1600 MPN/100 mL, while samples AL1, AL2, and AL6 showed the lowest MPN value of <1.8 MPN/100 mL. The MPN method using LTB and EC Broth media was effective in detecting fecal coliform contamination in wastewater samples.

References

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Published

2026-06-14

Issue

Vol. 11 No. 2 (2026): April - Juni 2026

Section

Articles

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Copyright (c) 2026 Siti Nabila Nabila, Endang Sulistyarini Gultom

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How to Cite

ANALISIS FECAL COLIFORM PADA AIR LIMBAH MENGGUNAKAN METODE MOST PROBABLE NUMBER (MPN). (2026). Jurnal Biogenerasi, 11(2). https://doi.org/10.30605/xmcbmm96