POLA PRODUKSI LISTRIK Bacillus licheniformis strain KGEB16 AMOBIL PADA SISTEM MICROBIAL FUEL CELL (MFC)
DOI:
https://doi.org/10.30605/8cvprk22Keywords:
Bacillus licheniformis, electricity production, immobilization, microbial fuel cell, natrium alginatAbstract
Peningkatan kebutuhan energi berkelanjutan telah menunutut pengembangan Microbial Fuel Cell (MFC) sebagai teknologi alternatif. Penelitian ini bertujuan untuk menganalisis pola produksi listrik Bacillus licheniformis strain KGEB16 yang diamobilisasi pada sistem Microbial Fuel Cell (MFC). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan variasi konsentrasi natrium alginat (2%, 3%, 4%, 5%, dan 6%) serta lima ulangan. Proses amobilisasi sel dilakukan dengan metode entrapment menggunakan natrium alginat, sedangkan pengukuran tegangan listrik dilakukan setiap 2 jam selama periode inkubasi 24 jam. Data yang diperoleh dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Duncan (Duncan Multiple Range Test/DMRT) pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa pola produksi listrik mengalami peningkatan secara bertahap sejak awal inkubasi hingga mencapai puncak pada jam ke-20, kemudian menurun pada jam ke-22 hingga 24. Tegangan tertinggi diperoleh pada konsentrasi natrium alginat 5%, sedangkan tegangan terendah diperoleh pada konsentrasi 2%. Dengan demikian, dapat disimpulkan bahwa konsentrasi natrium alginat berpengaruh signifikan terhadap pola produksi listrik, dengan konsentrasi optimum sebesar 5% pada sistem MFC.
References
Hassanzadeh, M., Bakhshi, M., dan Gharibi, D. (2017). Immobilization of microorganisms for biotechnological applications. Journal of Applied Microbiology, 123(5), 1278–1290.
Indriyani, Y. A., & Efendi, R. (2025). Microbial fuel cells (MFC): Sebuah teknologi untuk biokonversi energi kimia pada limbah organik menjadi biolistrik. Sultra Journal of Mechanical Engineering (SJME), 4(1), 10-21.
Logan, B. E. (2009). Exoelectrogenic bacteria that power microbial fuel cells. Nature Reviews Microbiology, 7(5), 375-381.
Logan, B. E., Rossi, R., Ragab, A. A., & Saikaly, P. E. (2019). Electroactive microorganisms in bioelectrochemical systems. Nature Reviews Microbiology, 17(5), 307-319.
Logan, B.E., Hamelers, B., Rozendal, R., Schroder, U., Keller, J., Freguia, J., Aelterman, P., Verstraete, W., Rabaey, K. (2006). Microbial fuel cells: methodology and technology. Environ Sci Technol. 40:5181–5192.
Lovley, D. R. (2006). Microbial fuel cells: novel microbial physiologies and engineering approaches. Current opinion in biotechnology, 17(3), 327-332.
Maulina A. R. (2025). Amobilsasi Bakteri Termofilik Sebagai Microbial Fuel Cell Dalam Menghasilkan Biolistrik. Skripsi. Padang ID: Universitas Negeri Padang, Indonesia
Mesran, M, H., Mamat, S., Pang, Y, R., Hong, T, Y., Muneera Z, Ghazali, N, F,Md., Ali, Md, A., & Mahmood, N, A, N., (2014). Preliminary Studies on Immobilized Cells-Based Microbial Fuel Cell System on Its Power Generation Performance. Journal of Asian Scientific Research 4(8): 428–435
Nugroho, I., Amaliyah, R. I. U., & Kirom, M. R. (2020). Pengaruh lama pembusukan nasi basi dan rasio volume variasi substrat terhadap produksi energi listrik pada sistem MFC. e-Proceeding of Engineering, 7(1), 1230–1237.
Parkash, A. (2015). Design and Fabrication of a Double Chamber Microbial Fuel Cells for Voltage Generation from Biowaste. Bioprocessing & Biotechniques. 5 (8): 1-4.
Rabaey, K., & Verstraete, W. (2005). Microbial fuel cells: Novel biotechnology for energy generation. Trends in Biotechnology, 23(6), 291–298.
Riwayati, I., Hartati, I., Dan Kurniasari, L. (2012). Teknologi Imobilisasi Sel Mikroorganisme Pada Produksi Enzim Lipase. In Prosiding Seminar Sains Nasional Dan Teknologi 1(1).
Singh, R., Kumar, A., & Sharma, P. (2024). The Critical Role of Salt Bridge in Proton Transfer and Performance of Two-Chamber Microbial Fuel Cells. Journal of Bioelectrochemistry and Biofuel, 10(1), 1-10.
Sulistiyawati, I., Rahayu, N. L., & Purwitaningrum, F. S. (2020). Produksi biolistrik menggunakan Microbial Fuel Cell (MFC) Lactobacillus bulgaricus dengan substrat limbah tempe dan tahu. Majalah Ilmiah Biologi Biosfera: A Scientific Journal, 37(2), 112–117.
Summaiati, Titi. (2024). Amobilisasi Kultur Campuran Bakteri Termofilik Pada Variasi Konsentrasi Alginat Dalam Produksi Enzim Xilanase. Skripsi. Padang: Universitas Negeri Padang.
Uthami, F. N., & Irdawati. (2024). Analisis karakteristik pola pertumbuhan bakteri termofilik isolat MS-17 dari sumber air panas Mudiak Sapan. Jurnal Tambusai, 8(2), 17109–17114.
Yuliana, N., Sarkono, Hidayati, E., & Faturrahman. (2022). Isolasi, karakterisasi, dan identifikasi Bacillus spp. berasosiasi Abalon (Haliotis asinina). Samota Journal of Biological Sciences 1(1): 1–10.
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