EXPLORATION AND IDENTIFICATION OF MICROBES AND FUNGI FROM SOIL SAMPLES OF GUNGGUNG VILLAGE, BATUAN DISTRICT, SUMENEP REGENCY AS BIOLOGICAL CONTROL AGENTS AT THE EAST JAVA FOOD CROPS AND HORTICULTURE PLANT PROTECTION LABORATORY  

Authors

  • Diva Anindita Darma Universitas Islam Negri Sunan Ampel Surabaya
  • Wiwin Misnati Nur Indahsari Universitas Islam Negeri Sunan Ampel Surabaya image/svg+xml

DOI:

https://doi.org/10.30605/b4ev2f20

Keywords:

Identification, Microbes, Fungi, Biological Agents

Abstract

Soil microorganisms play an essential role in maintaining soil fertility and suppressing plant pathogens, making them potential biological control agents in sustainable agriculture. This study aimed to explore and identify microbes and fungi from soil samples collected from yellow Kepok banana (Musa spp.) plantations in Gunggung Village, Batuan District, Sumenep Regency, as potential biological agents. The novelty of this research lies in the exploration of untreated Fusarium-infected banana plantation soil to obtain indigenous microbial isolates with biocontrol potential. A descriptive exploratory research design was employed. Bacterial isolation was conducted using the serial dilution method on Potato Dextrose Agar (PDA), followed by purification, macroscopic and microscopic characterization, 5% KOH test, soft rot test, and hypersensitivity test. Fungal isolation was performed using the baiting method with coconut, rice, and mealworms as substrates, followed by morphological identification. The results revealed the presence of Bacillus sp. as the dominant bacterial isolate, characterized by circular colonies, yellowish-white color, Gram-positive reaction, and rod-shaped cells. Soft rot and hypersensitivity assays indicated varying pathogenic responses among isolates. Fungal identification showed the presence of Aspergillus sp. (brownish-green, powdery colonies) and Penicillium sp. (yellowish-green, granular colonies). These findings indicate that soil from the study site contains microbial populations with potential applications as biological control agents. The study highlights the importance of exploring indigenous soil microorganisms to support environmentally friendly plant disease management and sustainable agricultural practices.

References

Abdelaziz, A. M., Hashem, A. H., El, G. S., Wakil, D. A. El, & Selim, S. (2023). Biocontrol of soil borne diseases by plant growth promoting rhizobacteria. Tropical Plant Pathology. https://doi.org/10.1007/s40858-022-00544-7

Agastya, I. M. I., Ameliawati, P., & Fikrinda, W. (n.d.). Eksplorasi dan identifikasi Jamur Patogen Serangga di Rhizosfer Lahan Kering Kabupaten Malang Exploration and identification of Insect Pathogenic Fungi in Rhizosphere on Dry Land Malang Regency. 17(3), 13–17.

Agensia, L. S., & Hayati, P. (2023). EKSPLORASI BAKTERI Bacillus spp . PADA PERAKARAN TANAMAN KENTANG Fusarium sp . ASAL LAHAN WONOKITRI KABUPATEN PASURUAN JAWA TIMUR EXPLORATION OF BACTERIA Bacillus spp . ON THE ROOTINGS OF POTATO PLANT ( Solanum tuberosum L .) AS A BIOLOGICAL CONTROL AGENT FOR THE PATHOGEN Fusarium sp . ORIGIN OF WONOKITRI LAND , PASURUAN DISTRICT ,. 25(4), 4028–4041.

Aji, W., Dion, R., & Fa, M. (2021). Isolasi dan Karakterisasi Kapang Filoplan serta Serasah Daun di Lingkungan Laboratorium Biologi Universitas Diponegoro Dengan Metode Contact Plate Isolation and Characterization of Filoplan Mold and Leaf Litter in the Biology Laboratory of Diponegoro University Using the Contact Plate Method. 23(1)

Aktivitas, U., Hasil, A., Bakteri, F., Umbi, E., Purwaningsih, D., & Wulandari, D. (2021). Jurnal Sains dan Kesehatan. 3(5), 750–759.

Amalia, N. (2013). IDENTIFIKASI JAMUR Aspergillus flavus PADA KACANG TANAH (Arachis hypogaea L ) YANG DIJUAL DI PASAR KODIM. 1, 1–10.

Diarta I Made, Javandira2, C., & Widnyan, * dan I Ketut. (2016). Fusarium oxysporum. 05(01).

Dohare, K. S., Lahagu, M. P., Novi, P., & Waruwu, K. (2025). Peran Mikroorganisme Tanah dalam Meningkatkan Kesehatan Tanah dan Hasil Pertanian Organik. 2.

Efikasi, U. J. I., Hayati, A., Penyakit, T., & Daun, H. (2013). UJI EFIKASI AGENS HAYATI TERHADAP PENYAKIT HAWAR DAUN BAKTERI (Xanthomonas oryzae pv. oryzae) PADA BEBERAPA VARIETAS PADI SAWAH (Oryza sativa). 1(4), 1402–1412.

Eko Famuji Ariyanto, Abdul Latief Abadi, S. D. (2013). Keanekaragaman Jamur Endofit Pada Daun Tanaman Padi (Oryza Sativa L.) Dengan Sistem Pengelolaan Hama Terpadu (PHT) Dan Konvensional Di Desa Bayem, Kecamatan Kasembon, Kabupaten Malang. 1, 37–51.

Ekwueme, A., & Alike, N. (2024). Application of Biosolids-Derived Biochar for the Remediation of Australian Soil Contaminated with Diesel Charles Chinyere Dike.

Hardiansyah, M. Y., Musa, Y., Jaya, A. M., Growth, P., & Rhizobacteria, P. (2020). Identifikasi Plant Growth Promoting Rhizobacteria pada Rizosfer Bambu Duri dengan Gram KOH 3 %. 4(1), 41–46. https://doi.org/10.20961/agrotechresj.v4i1.40875

Heikal, J. (2024). MOTIVASI PETANI MUDA DALAM PENGGUNAAN AGENS HAYATI TRICHODERMA SPP . PADA BUDIDAYA PERTANIAN. 2(1), 1366–1379.

Husen, S., Ishartati, E., Ruhiyat, M., Juliati, R., Malang, U. M., Nasional, S., & Pengabdian, H. (2018). Produksi benih kentang melalui teknik kultur in vitro. September, 274–280.

Khairani, M., Sukma, A., Damanik, H., & Lubis, I. R. (2025). Analisis Literatur Jurnal Pada Variasi Pengendalian Mikroorganisme. 3(1), 414–422.

Lea, V. C., Hamakonda2), U. A., & Enga, V. A. P. 3) I. T. 4) A. H. P. (2023). Identifikasi penyakit penting pada tanaman pisang di desa foa kecamatan aimere kabupaten ngada. 1, 45–49.

Lestari, S. A., Kulsum, U., & Ramdan, E. P. (2021). Efikasi Beberapa Agens Hayati Terhadap Penekanan Pertumbuhan Pyricularia grisea Secara In Vitro The Efficacy of Several Biological Agents Against In Vitro Growth Suppression of Pyricularia agrisea. 23(1), 31–36.

Made, K. (2023). Eksplorasi dan Identifikasi Bacillus sp . dari Tanah Rizosfer Bambu dan. 1(1), 1–6.

Mahendra, M. I., Martosudiro, M., & Choliq, F. A. (2022). EKSPLORASI JAMUR TANAH YANG BERPOTENSI SEBAGAI BIOREMEDIATOR FUNGISIDA BERBAHAN AKTIF PROPINEB PADA TANAMAN JERUK ( Citrus reticulata L.). 10(4), 174–186. https://doi.org/10.21776/ub.jurnalhpt.2022.010.4.3

Marsaoli, F., & Matinahoru, J. M. (2019). Isolasi , Seleksi , dan Uji Antagonis Bakteri Endofit diisolasi dari Salawaku ( Falcataria mollucana ) dalam Menekan Pertumbuhan Cendawan Patogen Cercospora spp . Isolation , Selection , and Antagonis Test of Endophytic Bacteria Isolated from Falcataria mollucana to Inhibit Growth of Cercospora spp .

Nyongesa, B. W., Okoth, S., & Ayugi, V. (2015). Identification Key for Aspergillus Species Isolated from Maize and Soi.

Putu, N., Ristiari, N., Srie, K., Julyasih, M., Ayu, I., Suryanti, P., & Biologi, J. (2018). ISOLASI DAN IDENTIFIKASI JAMUR MIKROSKOPIS PADA RIZOSFER TANAMAN JERUK SIAM ( Citrus nobilis Lour .) DI KECAMATAN KINTAMANI , BALI. 6(1), 10–19.

Sakiah, S., Arfianti, D., Silalahi, A. B., & Lesmana, I. (2024). Pemanfaatan Trichoderma sp dan Aspergillus sp dalam Pengomposan Tandan Kosong Kelapa Sawit. 2017.

Sari1, A. R., Kusdiyantini2, E., & MG Isworo Rukmi2. (2017). PRODUKSI SELULASE OLEH KAPANG Aspergillus sp. HASIL ISOLASI DARI LIMBAH PENGOLAHAN SAGU (Metroxylon sp.) DENGAN VARIASI KONSENTRASI INOKULUM PADA FERMENTASI TERENDAM STATIS. 6(1).

Serealia, O. P. T., Desa, D. I., & Kecamatan, S. (2023). Pkm teknik pengendalian organisme pengganggu tumbuhan (opt) serealia di desa senaning kecamatan pemayung. 1, 481–486. https://doi.org/10.57254/eka.v2i2.72

Sutanto, A., Widowati, H., Septiana, N., Thresia, F., & Hendri, N. (2023). Organic Vegetable Business Empowerment of Hijau Daun Farmers Group , Karang Rejo , North Metro Pemberdayaan Usaha Sayuran Organik Kelompok Tani Hijau Daun Karang Rejo Metro Utara. 4(1).

Triwidodo, H., Tondok, E. T., & Shiami, A. (2020). Pengaruh Varietas dan Umur Tanaman Berbeda terhadap Jumlah Populasi dan Tingkat Serangan Hama dan Penyakit Pisang ( Musa sp .) di Kabupaten Sukabumi. 31(2), 68–75.

Udayana, U., Mikrobiologi, L., & Udayana, U. (2022). Journal of Biological Sciences. 9(September), 325–337. https://doi.org/10.24843/metamorfosa.2022.v09.i02.p12

Umboh, S. D., Rondonuwu, F. B., Palar, D. C., Langoy, M. L. D., & Montong, V. B. (2024). PKM Manfaat Jamur Antagonis bagi Peningkatan Hasil Pertanian Desa Ongkaw Dua Kecamatan Sinonsayang Kabupaten Minahasa Selatan. 5(2).

Wisda, H., Sediawan, W. B., Teknik, M., Pencemaran, P., Kimia, D. T., Teknik, F., & Mada, U. G. (2016). Pengaruh aerasi pada fermentasi padat tandan kosong kelapa sawit oleh aspergillus niger terhadap produksi gula sederhana. 5(3), 12–16.

Wulandari, N., Irfan, M., & Saragih, R. (2019). ISOLASI DAN KARAKTERISASI PLANT GROWTH PROMOTING RHIZOBACTERIA DARI RIZOSFER KEBUN KARET RAKYAT Isolation and Characterization of Plant Growth Promoting Rhizobacteria from the Rizosphere of Folk Rubber Plantations. 2019, 57–64.

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Published

2026-03-14

Issue

Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)

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Copyright (c) 2026 Diva Anindita Darma, Wiwin Misnati Nur Indahsari

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How to Cite

EXPLORATION AND IDENTIFICATION OF MICROBES AND FUNGI FROM SOIL SAMPLES OF GUNGGUNG VILLAGE, BATUAN DISTRICT, SUMENEP REGENCY AS BIOLOGICAL CONTROL AGENTS AT THE EAST JAVA FOOD CROPS AND HORTICULTURE PLANT PROTECTION LABORATORY  . (2026). Jurnal Biogenerasi, 11(1), 321-333. https://doi.org/10.30605/b4ev2f20

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