KEMAMPUAN PSEUDOMONAS BERFLUORESEN SEBAGAI AGEN ANTAGONIS TERHADAP Ralstonia solanaceaerum PENYEBAB LAYU TANAMAN NILAM (Pogestemon cablin Benth)
DOI:
https://doi.org/10.30605/biogenerasi.v11i1.8088Keywords:
Patchouli, Ralstonia solanaceaerum, Bacterial wilt disease, Fluoresent PseudomonasAbstract
Patchouli (Pogestemon cablin Benth) is a cultivated plant that can produce oil and generate income for farmers. However, attacks by plant pests (OPT) often pose a challenge to patchouli production in Indonesia. One organism that causes wilt disease in patchouli plants is the bacterium Ralstonia solanaceaerum. Therefore, more environmentally friendly and sustainable control methods are needed. One strategy is the use of biological agents, especially antagonistic rhizosphere bacteria such as fluorescent pseudomonas. This study was conducted from June to August 2025 at the Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study used a completely randomized design (CRD) as the experimental design. In this study, there were 6 treatments (different isolates), each repeated 3 times. The parameter observed was the formation of an inhibition zone using the disc diffusion method. The data were analyzed using analysis of variance (ANOVA). If there were significant differences, Duncan's New Multiple Range Test (DNMRT) was used at a 5% level. Based on the results and discussions, it can be concluded that all fluorescent Pseudomonas isolates tested have the ability to inhibit R. solanaceaerum, the cause of wilt disease in patchouli plants. The isolate with the best ability to inhibit the growth of R. solanaceaerum on patchouli plants was isolate Pf S36 with an inhibition zone of 20.37 mm, but there was no significant difference between the other isolates because the inhibition effectiveness was relatively uniform.
Downloads
References
Ababa, G. 2024. Pathogenic Diversity, Ecology, Epidemiology, and Management Practices of the Potato Cavterial Wilt (Ralstonia solanaceaerum) Disease. Journal Cogent Food & Agriculture, 10(1): 1-18.
Advinda, L. 2020. Pseudomonad Fluoresen Agens Biokontrol Blood Disease Bacteria (BDB) Tanaman Pisang, (Monograf). Yogyakarta: Deepublish.
Febriani, I., Advinda, L., Handayani, D., Farma, S. a., Putri, D. H. 2023. Asosiasi Pseudomonad Fluoresen pada Rizosfir Tanaman. Serambi Biologi, 8(2), 117-122.
Gabriel, D.W., Allen, I.C., Schell, M., Denny, T.P., Greenberg, J.T., Duan, Y.P., Cruz, Z. F., Huang, Q., Clifford, J. M., Presting, G., González, E.T., Reddy, J., Elphinstone, J., Swanson, J., Yao, J., Mulholland, V., Liu, L., Farmerie, W., Patnaikuni, M., Balogh, B., Norman, I.D., Alvarez, A., Castillo, J.A., Jones, J., Saddler, G., Walunas,T., Zhukov, A., Mikhailova, N. 2006. Identification of Open Reading Frames Unique to a Select Agent: Ralstonia solanacearum Race 3 Biovar 2. Molecular Plant-Microbe Interactions, 19 (1): 69-79.
Glick, B. R. 2012. Plant Growth-promoting Bacteria: Mechanisms and Applications. Scientifica, 2012, Article ID 963401.
Haas, D., & Défago, G. 2005. Biological Control of Soil-borne Pathogens by fluorescent Pseudomonads. Nature Reviews Microbiology, 3(4): 307–319.
Harli. 2016. Identifikasi dan Potensi Perluasan Tanaman Nilam (Pogestemon cablin Benth) di Bawah Tegakan Kakao di Kabupaten Polewali Mandar. Jurnal Agrovital, 1(1): 21-26.
Hasanuddin. 2011. Uji Aktivitas Antibiosis Pseudomonads Pendarfluor Terhadap Rigidoporus lignosus (Klotszch) imazeki Penyebab Penyakit Akar Putih. Jurnal HPT Tropika, 11(1): 87-94.
Istiqomah & Kusumawati, E. 2018. Pemanfaatan Bacillus subtilis dan Pseudomonas fluorescens dalam Pengendalian Hayati Ralstonia solanacearum Penyebab Penyakit Layu Bakteri pada Tomat. Jurnal Agroindustri, 5(1): 1-12.
Khairan, K., Husna, N., Maisyarah, H., & Diah, M. 2025. Formulation and Evaluastion of Liquid Perfum from Natural Fragrance Using Patchouli Oil. Indonesian Journal of Pharmaceutical Science and Technology, 12 (2): 119-129.
Lingga, A. R., Pato, U., & Rossi, E. 2016. Uji Antibakteri Ekstrak Batang Kecombrang (Nicolaia speciosa horan) Terhadap Staphylococcus eureus dan Escherichia coli. Jurnal Jom Faperta, 3(1): 1-15.
Merta, I. W., Nuidja, I. N. & Marwati, N. M. 2013. Ektrak Gambir Memiliki Daya Hambat Terhadap Pertumbuhan Staphylococcus aureus secara in Vitro. Jurnal Skala Husada, 10(1): 39-43.
Mesy, M. AR., Lestari, M. D., Setiawan, U. N., Setyaningrum, E., Nukmal, N., Arifyianto, & A., Aeny, T. N. 2022. Uji Daya Hambat Pertumbuhan Mikroba Patogen oleh Streptomyces sp. strain 188 Sebagai Agen Biokontrol. Jurnal Bioekperimen, 8(2): 88-96.
Nasrun & Nurmansyah. 2016. Keefektifan Formula Pseudomonas fluorescens untuk Mengendalikan Penyakit Layu Bakteri dan Meningkatkan Pertumbuhan Tanaman Nilam. Jurnal Fitopatologi Indonesia, 12 (2): 46-52.
Nur. M. S., Mohd, Z. A. W., & Sapak, Z. 2023. The Potential of Pseudomonas fluorescens as Biological Control Agent Against Sheath Blight Disease in Rice: a Systematic review. Journal Food Research, 7(2): 46-56.
Rambe, T. R., Parinduri, W. M., Wandi, L., Nasir, M., Sulaimant, Herdiani, E., Maharani, E. P., & Sunita. 2022. Pemanfaatan Minyak Atsiri Daun Nilam untuk Mengobati Sakit Kepala. Jurnal Pengabdian Kepada Masyarakat, 3(2): 62-68.
Soesanto, L., Mugiastuti, E., & Rahayuniati, R.F. 2010. Kajian Mekanisme Antagonis Pseudomonas Fluorescens P60 terhadap Fusarium oxysporum, Fusarium sp. Lycopersici pada Tanaman Tomat in Vivo. Jurnal Hama Penyakit Tumbuhan Tropis, 10(2): 108-115.
Setiawan, A.W. 2019. Epidomologi Penyakit Layu Bakteri dan Perkembangan Kompleks Spesies Ralstonia solanacearum. Jurnal Galung Tropika, 8(3): 243-270.
Weller, D. M. 2007. Pseudomonas Biocontrol Agents of Soilborne Pathogens: Looking Back Over 30 Years. Jurnal Phytopathology, 97(2): 250–256.
Yuliar, Nion, Y. A., & Toyota, K. 2015. Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Jurnal Microbes and environments, 30(1): 1-11.
Zehra, A., Raytekar, N. A., Meena, M., & Swapnil, P. 2024. Efficiency of Microbial Bio-agents as Elicitors in Plant Defense Mechanism Under Biotic Stress: A Review. Current Research in Microbial Sciences, vol, 2.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Abil Pratama Putra, Linda Advinda, Moralita Chatri, Dezi Handayani
This work is licensed under a Creative Commons Attribution 4.0 International License.
In submitting the manuscript to the journal, the authors certify that:
- They are authorized by their co-authors to enter into these arrangements.
- The work described has not been formally published before, except in the form of an abstract or as part of a published lecture, review, thesis, or overlay journal.
- That it is not under consideration for publication elsewhere,
- That its publication has been approved by all the author(s) and by the responsible authorities – tacitly or explicitly – of the institutes where the work has been carried out.
- They secure the right to reproduce any material that has already been published or copyrighted elsewhere.
- They agree to the following license and copyright agreement.
License and Copyright Agreement
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
.png)
