INTERAKSI SINERGIS DALAM KONSORSIUM TERMOFILIK: MENYEIMBANGKAN RASIO KONSENTRASI MIKROBA DALAM PRODUKSI BIOELEKTRIK
DOI:
https://doi.org/10.30605/biogenerasi.v10i2.6048Keywords:
Microbial Fuel Cell, thermophilic consortium, inoculum ratio, bioelectricity, synergistic interactionAbstract
The global urgency for sustainable energy solutions amid climate change and fossil fuel depletion has driven innovation in Microbial Fuel Cells (MFCs). This study investigates the synergistic interactions within a thermophilic microbial consortium (MS9 & MS12) to optimize energy efficiency in MFCs. By varying the inoculum ratios (1:1, 1:2, and 1:3), the research evaluates how microbial population balance impacts bioelectricity production. The MFCs were operated for 24 hours, with voltage measurements recorded every 2 hours. Results revealed that a 1:3 (MS9:MS12) ratio achieved the highest voltage output, peaking at 20 hours before declining, indicating metabolic synergy and efficient substrate utilization. Descriptive analysis highlighted the critical role of cross-feeding mechanisms and functional complementarity between species in enhancing electron transfer. The findings align with modular co-cultivation principles, emphasizing the importance of engineered microbial ratios for stable system performance. This study provides a protocol for designing balanced thermophilic consortia, advancing sustainable bioenergy strategies through optimized microbial interactions.
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References
Cendra, A. S. (2022, December 22). ESDM Ungkap Konsumsi Energi Fosil Masih Dominan, EBT Masih Jauh dari Target. Retrieved from Detik Finance: https://finance.detik.com/energi/d-6475412/esdm-ungkap-konsumsi-energi-fosil-masih-dominan-ebt-masih-jauh-dari-target
Christwardana, M., Joelianingsih, J., & Yoshi, L. A. (2023). Synergistic of yeast Saccharomyces cerevisiae and glucose oxidase enzyme as co-biocatalyst of enzymatic Microbial Fuel Cell (EMFC) in converting sugarcane bagasse extract into electricity. Journal of Electrochemical Science and Engineering, 13(2), 321–332. DOI: https://doi.org/10.5599/jese.1559
Deng, Y. J., & Wang, S. Y. (2016). Synergistic growth in bacteria depends on substrate complexity. Journal of Microbiology, 54(1):23-30. DOI: 10.1007/s12275-016-5461-9
Jawed, K., Yazdani, S. S., & Koffas, M. A. (2019). Advances in the development and application of microbial consortia for metabolic engineering. In Metabolic Engineering Communications, 9:1-10. DOI: https://doi.org/10.1016/j.mec.2019.e00095
Kostylev, M., & Wilson, D. (2012). Synergistic interactions in cellulose hydrolysis. Biofuels, 3(1) :61-70. DOI: 10.4155/bfs.11.150
Lahope, G. (2024). Implementasi Kebijakan Energi Nasional (KEN) Indonesia Menuju 23% Target Bauran Energi Baru Terbarukan (EBT) 2025. Jurnal Darma Agung, 32(1), 124-135. https://jurnal.darmaagung.ac.id/index.php/jurnaluda/article/download/3945/3607
Ning, X., Huang, Y., Huang, P., Ou, X., Luo, H., Bai, Z., Chen, H., Ge, X., & Li, L. (2024). Effect of the inoculum mixing ratio on the anaerobic digestion of food waste: Reactor performance and microbial community. Environmental Technology and Innovation, 35. DOI: https://doi.org/10.1016/j.eti.2024.103680
Risna, Y. K., Sri-Harimurti, S. H., Wihandoyo, W., & Widodo, W. (2022). Kurva pertumbuhan isolat bakteri asam laktat dari saluran pencernaan itik lokal asal aceh. Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science), 24(1), 1-7. https://jpi.faterna.unand.ac.id/index.php/jpi/article/download/718/446
Rolfe, M. D., Rice, C. J., Lucchini, S., Pin, C., Thompson, A., Cameron, A. D., ... & Hinton, J. C. (2012). Lag phase is a distinct growth phase that prepares bacteria for exponential growth and involves transient metal accumulation. Journal of bacteriology, 194(3):686-701. DOI: 10.1128/JB.06112-11
Purnama, T., & Sanantang. (2023). -UJI SKRINING FITOKIMIA EKSTRAK SUPERNATAN DARI BAKTERI ENDOFIT KULIT PISANG. Bioma: Jurnal Biologi Makassar, 8(1):44-50. https://journal.unhas.ac.id/index.php/bioma/article/view/23545/8882
Saraswati, P. W., Nocianitri, K. A., & Arihantana, N. M. I. H. (2021). Pola Pertumbuhan Lactobacillus sp. F213 Selama Fermentasi Pada Sari Buah Terung Belanda (Solanum betaceum Cav.) Growth Pattern of Lactobacillus sp. F213 During Fermentation in Tamarillo Juice (Solanum betaceum Cav.). Itepa: Jurnal Ilmu dan Teknologi Pangan, 10(4):621-633. https://ojs.unud.ac.id/index.php/itepa/article/download/82624/42896
Srivastava, R. K., Boddula, R., & Pothu, R. (2022). Microbial Fuel Cells: Technologically advanced devices and approach for sustainable/renewable energy development. Energy Conversion and Management: X, 13. https://doi.org/10.1016/j.ecmx.2021.100160
Sulistiyawati, I., Laila Rahayu, N., & Septiana Purwitaningrum, F. (2020). Produksi Biolistrik menggunakan Microbial Fuel Cell (MFC) Lactobacillus bulgaricus dengan Substrat Limbah Tempe dan Tahu. Majalah Ilmiah Biologi Biosfera : A Scientific Journal,37(2):112-117. DOI: https://doi.org/10.20884/1.mib.2020.37.2.1147
Wetser K., Sudirjo E., Buisman C. J. N. & Strik D. P. B. T. B. (2015). Electricity generation by a plant Microbial Fuel Cell with an integrated oxygen reducing biocathode. Applied Energy, 137, 151–157. DOI: https://doi.org/10.1016/j.apenergy.2014.10.006
Wintermute, E. H., & Silver, P. A. (2010). Emergent cooperation in microbial metabolism. Molecular systems biology, 6(407): 1-7. DOI: https://doi.org/10.1038/msb.2010.66
Christwardana, M., Joelianingsih, J., & Yoshi, L. A. (2023). Synergistic of yeast Saccharomyces cerevisiae and glucose oxidase enzyme as co-biocatalyst of enzymatic Microbial Fuel Cell (EMFC) in converting sugarcane bagasse extract into electricity. Journal of Electrochemical Science and Engineering, 13(2), 321–332. DOI: https://doi.org/10.5599/jese.1559
Deng, Y. J., & Wang, S. Y. (2016). Synergistic growth in bacteria depends on substrate complexity. Journal of Microbiology, 54(1):23-30. DOI: 10.1007/s12275-016-5461-9
Jawed, K., Yazdani, S. S., & Koffas, M. A. (2019). Advances in the development and application of microbial consortia for metabolic engineering. In Metabolic Engineering Communications, 9:1-10. DOI: https://doi.org/10.1016/j.mec.2019.e00095
Kostylev, M., & Wilson, D. (2012). Synergistic interactions in cellulose hydrolysis. Biofuels, 3(1) :61-70. DOI: 10.4155/bfs.11.150
Lahope, G. (2024). Implementasi Kebijakan Energi Nasional (KEN) Indonesia Menuju 23% Target Bauran Energi Baru Terbarukan (EBT) 2025. Jurnal Darma Agung, 32(1), 124-135. https://jurnal.darmaagung.ac.id/index.php/jurnaluda/article/download/3945/3607
Ning, X., Huang, Y., Huang, P., Ou, X., Luo, H., Bai, Z., Chen, H., Ge, X., & Li, L. (2024). Effect of the inoculum mixing ratio on the anaerobic digestion of food waste: Reactor performance and microbial community. Environmental Technology and Innovation, 35. DOI: https://doi.org/10.1016/j.eti.2024.103680
Risna, Y. K., Sri-Harimurti, S. H., Wihandoyo, W., & Widodo, W. (2022). Kurva pertumbuhan isolat bakteri asam laktat dari saluran pencernaan itik lokal asal aceh. Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science), 24(1), 1-7. https://jpi.faterna.unand.ac.id/index.php/jpi/article/download/718/446
Rolfe, M. D., Rice, C. J., Lucchini, S., Pin, C., Thompson, A., Cameron, A. D., ... & Hinton, J. C. (2012). Lag phase is a distinct growth phase that prepares bacteria for exponential growth and involves transient metal accumulation. Journal of bacteriology, 194(3):686-701. DOI: 10.1128/JB.06112-11
Purnama, T., & Sanantang. (2023). -UJI SKRINING FITOKIMIA EKSTRAK SUPERNATAN DARI BAKTERI ENDOFIT KULIT PISANG. Bioma: Jurnal Biologi Makassar, 8(1):44-50. https://journal.unhas.ac.id/index.php/bioma/article/view/23545/8882
Saraswati, P. W., Nocianitri, K. A., & Arihantana, N. M. I. H. (2021). Pola Pertumbuhan Lactobacillus sp. F213 Selama Fermentasi Pada Sari Buah Terung Belanda (Solanum betaceum Cav.) Growth Pattern of Lactobacillus sp. F213 During Fermentation in Tamarillo Juice (Solanum betaceum Cav.). Itepa: Jurnal Ilmu dan Teknologi Pangan, 10(4):621-633. https://ojs.unud.ac.id/index.php/itepa/article/download/82624/42896
Srivastava, R. K., Boddula, R., & Pothu, R. (2022). Microbial Fuel Cells: Technologically advanced devices and approach for sustainable/renewable energy development. Energy Conversion and Management: X, 13. https://doi.org/10.1016/j.ecmx.2021.100160
Sulistiyawati, I., Laila Rahayu, N., & Septiana Purwitaningrum, F. (2020). Produksi Biolistrik menggunakan Microbial Fuel Cell (MFC) Lactobacillus bulgaricus dengan Substrat Limbah Tempe dan Tahu. Majalah Ilmiah Biologi Biosfera : A Scientific Journal,37(2):112-117. DOI: https://doi.org/10.20884/1.mib.2020.37.2.1147
Wetser K., Sudirjo E., Buisman C. J. N. & Strik D. P. B. T. B. (2015). Electricity generation by a plant Microbial Fuel Cell with an integrated oxygen reducing biocathode. Applied Energy, 137, 151–157. DOI: https://doi.org/10.1016/j.apenergy.2014.10.006
Wintermute, E. H., & Silver, P. A. (2010). Emergent cooperation in microbial metabolism. Molecular systems biology, 6(407): 1-7. DOI: https://doi.org/10.1038/msb.2010.66
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Published
2025-06-02
How to Cite
Alfatimah Azzahra, B. ., Irdawati, Handayani, D. ., & Hilda Putri, D. . (2025). INTERAKSI SINERGIS DALAM KONSORSIUM TERMOFILIK: MENYEIMBANGKAN RASIO KONSENTRASI MIKROBA DALAM PRODUKSI BIOELEKTRIK. Jurnal Biogenerasi, 10(2), 1483–1489. https://doi.org/10.30605/biogenerasi.v10i2.6048
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