IDENTIFIKASI BAKTERI LAUT SECARA MOLEKULER DARI OBJEK WISATA PUSAT LAUT KABUPATEN DONGGALA SULAWESI TENGAH

Authors

  • Slamet Ifandi
  • Astri Febriana Iffaf Politeknik Cendrawasih Palu

DOI:

https://doi.org/10.30605/biogenerasi.v10i2.6054

Keywords:

Marine Bacteria, Molecular, Marine Center Tourism Object

Abstract

Research in marine areas related to the potential of marine bacteria in Indonesia has been widely conducted. Information on bacterial research originating from the waters of Central Sulawesi, especially Donggala Regency, is still very rare and limited to observations of isolation and characterization. Therefore, a more accurate identification method is needed, namely using molecular. Molecular identification is considered more accurate when compared to other identification methods. This study aims to identify marine bacteria molecularly through the 16S rRNA gene. The method used in this study is experimental with a descriptive approach. The results of the molecular identification of bacterial isolates showed that isolate PS4 had the closest kinship level to Pseudomonas stutzeri. Isolate PS5 has a kinship with Bacillus megaterium and isolate PS6 has the closest kinship to Pseudomonas aeruginosa. All levels of kinship with those obtained were 100% percent identity.

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References

Ifandi S., Nugraini S., dan Enggar.2025.Potensi Bakteri Pseudomonas stutzeridari Objek Wisata Pusat Laut, Kabupaten Donggala sebagai Antibakteri terhadap Staphylococcus aureus.Jurnal Medika Nusantara.Vol.3(1):1-5.
Mardiana, N.A., Muniasih, T., Rukmi, W.D., dan Kusnadi, J. 2020. Potensi Bakteri Laut Sebagai SumberAntibiotik baru penghambat Saccharomyces Aureus. Jurnal Teknologi Pertanian.Vol. 21 (1):49-56.
Chrisnawati S.D., Sabdaningsih S., Jati O.E & Ayuningrum D.2023. Solasi Dan Identifikasi Molekuler Bakteri Rhizosfer Dari Sedimen Mangrove Jenis Rhizopora Sp. Di Ekosistem Mangrove Tapak, Semarang. Jurnal Kelautan. Vol.16(2):117-124.
Noer S.2021.Identifikasi Bakteri secara Molekular Menggunakan 16S rRNA. EduBiologia. Vol. 1(1):2-6.
Nugraini, S., Permatasari, G., Mutasin, K., Humairah, Ifandi, S., & Gintoe, H. L. (2024). Solasi dan karakterisasi bakteri laut dari objek wisata pusat laut desa Towale Kabupaten Donggala Sulawesi Tengah. Jurnal Pendidikan Biologi (Biogenerasi), 1(1):1–6.
Rau C.H.. Yudistira A., dan Simbala H.E. I.2018. Isolasi, Identifikasi Secara Molekuler Menggunakan Gen 16s rRNA, Dan Uji Aktivitas Antibakteri Bakteri Simbion Endofit Yang Diisolasi Dari Alga (Halimeda Opuntia). PHARMACON: Jurnal Ilmiah Farmasi. Vol. 7(2).53-61.
Sogandi. Biologi Molekuler Identifikasi Bakteri Secara Molekuler.2019. Penerbit : Universitas 17 Agustus 1945 Jakarta.ISBN. 978-602-53782-1-8
Stackebrandt, E., dan Goebel, B. M. (1994). Taxonomic Note : A Place for DNADNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology. International Journal of Systematic Bacteriology, 44(4), 846–849. https://doi.org/0020-7713/94/$04.00+0
Sune, D., Rydberg, H., Augustinsson, A. N., Serrander, L. dan Jungestrom, M. B. (2020). Optimization of 16S rRNA gene Analysis for Use in the Diagnostic Clinical Microbiology Service. Journal of Microbiological Methods, 170, 1-8. https://doi.org/10.1016/j.mimet.2020.1 05854.

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Published

2025-06-20

How to Cite

Ifandi, S. ., & Febriana Iffaf, A. . (2025). IDENTIFIKASI BAKTERI LAUT SECARA MOLEKULER DARI OBJEK WISATA PUSAT LAUT KABUPATEN DONGGALA SULAWESI TENGAH. Jurnal Biogenerasi, 10(2), 1533–1536. https://doi.org/10.30605/biogenerasi.v10i2.6054

Issue

Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)

Section

Articles

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