AMPLIFIKASI GEN COI PADA SAMPEL LEBAH TANPA SENGAT DI LAMPUNG TENGAH: ANALISIS SECARA KUALITATIF
DOI:
https://doi.org/10.30605/biogenerasi.v10i2.5539Abstract
Indonesia memiliki keanekaragaman flora dan fauna yang tinggi dengan iklim tropis yang dimilikinya. Lebah tanpa sengat berhabitat di daerah tropis dan memiliki keanekaragaman yang tinggi dan menghasilkan produk madu dan propolis serta telah banyak dibudidayakan. Lampung Tengah sebagai salah satu daerah yang memiliki lokasi budidaya lebah tanpa sengat, dipilih sebagai lokasi penelitian. Penelitian telah dilakukan dengan analisis molekuler menggunakan penanda molekuler gen COI. Penelitian ini bertujuan untuk mengetahui kualitas hasil amplifikasi gen COI pada empat individu lebah tanpa sengat yang dikoleksi di Lampung Tengah. Analisis molekuler telah dilakukan melalui proses ekstraksi, amplifikasi, elektroforesis, dan visualisasi DNA. Kualitas DNA diketahui melalui pita DNA yang terbentuk pada proses elektroforesis gel agarosa 1% dan divisualisasikan di bawah sinar ultraviolet. Hasil amplifikasi gen COI pada empat individu lebah tanpa sengat memiliki kualitas pita DNA yang baik dengan panjang base pair berkisar antara 600 – 750bp.
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