Efektivitas Lactobacillus fermentum pada Fermentasi Kopi Arabika (Coffea arabica) dengan Media Rongga Batang Bambu Terhadap Kadar Kafein dan Antioksidan

The Effectiveness of Lactobacillus fermentum in the Fermentation of Arabica Coffee (Coffea arabica) with Bamboo Stem Cavity Media on Caffeine and Antioxidant Content

Authors

  • St. Chadijah Politeknik Pertanian Negeri Pangkep
  • Fatimah Fatimah Politeknik Pertanian Negeri Pangkep
  • Darmawan Darmawan Politeknik Pertanian Negeri Pangkep

DOI:

https://doi.org/10.30605/perbal.v14i1.8096

Keywords:

Kopi Arabika, fermentasi, Lactobacillus fermentum, bambu, kafein, antioksidan

Abstract

Fermentasi merupakan tahap krusial dalam proses pascapanen kopi yang menentukan mutu kimia dan sensorik biji kopi. Penelitian ini bertujuan mengevaluasi efektivitas Lactobacillus fermentum pada fermentasi kopi Arabika menggunakan media rongga batang bambu terhadap perubahan kadar kafein, dan aktivitas antioksidan. Penelitian menggunakan rancangan acak lengkap dengan empat perlakuan fermentasi, yaitu P0 (kontrol tanpa inokulasi), P1 (24 jam), P2 (48 jam), dan P3 (72 jam) dengan tiga ulangan. Parameter yang diamati meliputi pH fermentasi, kadar kafein, aktivitas antioksidan metode DPPH. Hasil penelitian menunjukkan bahwa fermentasi dengan Lactobacillus fermentum menurunkan pH hingga 4,2, memodifikasi kadar kafein, dan menurunkan aktivitas antioksidan seiring bertambahnya lama fermentasi yang ditunjukkan pada perlakuan P3 72 jam. Media rongga batang bambu sebagai wadah fermentasi berperan sebagai mikrohabitat alami yang mendukung fermentasi terkendali dan pembentukan kompleksitas cita rasa. Kombinasi Lactobacillus fermentum dan bambu berpotensi menjadi inovasi ramah lingkungan untuk peningkatan mutu kopi specialty Indonesia.

Fermentation is a crucial stage in the post-harvest process of coffee that determines the chemical and sensory quality of coffee beans. This study aims to evaluate the effectiveness of Lactobacillus fermentum in Arabica coffee fermentation using bamboo stem cavity media on changes in caffeine levels and antioxidant activity. The study used a completely randomized design with four fermentation treatments, namely P0 (control without inoculation), P1 (24 hours), P2 (48 hours), and P3 (72 hours) with three replications. The parameters observed included fermentation pH, caffeine levels, and antioxidant activity using the DPPH method. The results showed that fermentation with Lactobacillus fermentum reduced the pH to 4.2, modified caffeine levels, and decreased antioxidant activity as the fermentation time increased, as shown in the P3 72-hour treatment. The bamboo stem cavity media as a fermentation container acts as a natural microhabitat that supports controlled fermentation and the formation of flavor complexity. The combination of Lactobacillus fermentum and bamboo has the potential to be an environmentally friendly innovation for improving the quality of Indonesian specialty coffee.

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References

Avallone, S., Guyot, B., Brillouet, J. M., Olguin, E., & Guiraud, J. P. (2001). Microbiological and biochemical study of coffee fermentation. Journal of Food Science, 66, 147–154.

De Bruyn, F., Zhang, S. J., Pothakos, V., Torres, J., Lambot, C., Moroni, A. V., Callanan, M., Sybesma, W., Weckx, S., & De Vuyst, L. (2017). Exploring the impacts of postharvest processing on the microbiota and metabolite profiles during green coffee bean production. Appl Environ Microbiol, 83, e02398-16. https://doi.org/10.1128/AEM.02398-16

Elhalis H., Cox, J., & Zhao, J. (2020). Ecological diversity, evolution and metabolism of microbial communities in the wet fermentation of Australian coffee beans. International Journal of Food Microbiology, 321, 108544. https://doi.org/10.1016/j.ijfoodmicro.2020.108544

Farah, A. (2012). Coffee constituents. In: Coffee: Emerging Health Effects and Disease Prevention. Wiley-Blackwell.

Gokulakrishnan, S., Chandraraj, K., & Gummadi, S. N. (2005). Microbial and enzymatic changes during coffee fermentation. Critical Reviews in Food Science and Nutrition, 45(1), 1–10.

Haile, M., & Kang, W. H. (2019). The role of microbes in coffee fermentation and their impact on coffee quality. Hindawai: Journal of Food Quality, 2019, 1-6.

Hatiningsih, S., Permana, I. D. G. M., Harsojuwono, B. A., & Adi, I. B. W. G. N. W. (2022). Pengaruh penambahan Lactobacillus fermentum CK165 dan lama fermentasi terhadap karakteristik fisik kopi arabika (Coffea arabica) asal Kintamani, Bangli. Itepa: Jurnal Ilmu dan Teknologi Pangan, 11(3), 506-522.

Kansrini, Y., Febrimeli, D., & Mulyani, P. W. (2020). Tingkat adopsi budidaya yang baik (Good Agriculture Practices) tanaman kopi arabika oleh petani di Kabupaten Tapanuli Selatan. Jurnal Paradigma Agribisnis, 3(1), 36–49.

Lee, L. W., Cheong, M. W., Curran, P., Yu, B., & Liu, S. Q. (2015). Coffee fermentation and flavor. Journal of Agricultural and Food Chemistry, 63, 4614–4625.

Sugitha I. M., & Puspawati, N. N. (2018). Cows milk dadih made in dried petung bamboo bali. Scientific Journal of Food Technology,5(2), 80 - 84

Sunarharum, W., Davit J.,W., & Heather S. (2014). Complexity of coffee flavor: A compositional and sensory perspective. Food Research International, 62, 315-325.

Usman, H., Rahardjo, M., & Wahyudi, A. (2015). Kajian penggunaan bambu sebagai wadah fermentasi kopi Arabika secara alami. Jurnal Penelitian Tanaman Industri, 21(2), 75–82.

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Published

2026-02-20