IDENTIFIKASI DNA BARCODING TUMBUHAN BALAKKA (Phyllanthus emblica L.) BERDASARKAN LOKUS GEN rbcL
DOI:
https://doi.org/10.30605/biogenerasi.v10i1.5145Keywords:
DNA Barcoding, rbcL, Phyllanthus emblica, PhylogeneticAbstract
This study aims to identify DNA barcodes with the rbcL gene locus and reconstruct the phylogenetic relationship of the Balakka plant with species belonging to the genus Phyllanthus. This research includes experimental research by isolating DNA, amplification DNA using rbcL gene as primer, electrophoresis and sequencing. DNA was isolated with a commercial kit, amplification was carried out using the Polymerase Chain Reaction method. Sequence results and phylogenetic tree reconstruction were analyzed by Bioinformatics (MEGA 11). The results showed that the number of amplicons from the consensus of the two primers on the balakka plant sample was 557 bp. The phylogenetic tree of the Balakka sequence formed a sister clade with Phyllantus emblica KY9888320.1 with 66% similarity, and P. emblica grouped together, but still had common ancestor branches with other species of the Phyllantus genus using the Neighbor-Joining method, and Kimura 2-parameter calculations.
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References
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Sagala, L.R., Lazuardi, Harahap., F., Manalu, K., Idami, Z., & Prasetya, E. (2021). DNA Barcoding Zingiber loerzingii Valeton menggunakan lokus gen Ribulose-1,5- biphosphate Carboxylase-OxygenaseLarge subunit Gene (rbcL). Jurnal Biosains, 7(3), 166-173.
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Wang, D-Y., Wang, Q., Wang, Y-L., Xiang, X-G., Huang, L-Q., and Jin, X-H. (2017). Evaluation of DNA Barcodes in Codonopsis (Campanulaceae) and in some Large Angiosperm Plant Genera. PLoS ONE, 12(2), 1-14..
Xu, J., Zang, F., Wu, Q., Wang, Y., Wang, B., Huang, P., Zang, D., Ma, Y., & Zheng, Y. (2021). Analysis of the genetic diversity and molecular phylogeography of the endangered wild rose (Rosa rugosa) in China based on chloroplast genes. Global Ecology and Conservation, 28, 1-12.
Yan, M., Dong, S., Gong, Q., Xu, Q., & Ge, Y. (2023). Comparative chloroplast genome analysis of four Polygonatum species insights into DNA barcoding, evolution, and phylogeny. Scientific Reports, 13(1), 1–23.
Bakshi, P., Wali, V.K., Jasrotia, A., Bhushan, B., and Bakshi, M. (2016). Aonla Cultivation. Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu.
Basith A. (2015). Peluang Gen rbcL sebagai DNA Barcode Berbasis DNA Kloroplas untuk Mengungkap Keanekaragaman Genetik Padi Beras Hitam (Oryza sativa L.) Lokal Indonesia. Biologi, Sains, Lingkungan, dan Pembelajarannya, 1, 938-941.
Direktorat Warisan dan Diplomasi Budaya, Dirjend Kebudayaan, Kemendikbud. (2017). Penetapan Warisan Budaya Takbenda Indoensia Tahun 2017. Jakarta. Direktorat Warisan dan Diplomasi Budaya, Dirjend Kebudayaan, Kemendikbud.
Gustianty, L.R. (2018). Kajian Pustaka: Balakka (Phylanthus emblica L) sebagai Hasil Hutan Bukan Kayu yang Tidak Terkelola dengan Baik di Sumatera Utara. Jurnal Pionir, 2(5), 70-75.
Hall, B. G. (2018). Phylogenetic Tree Made Easy : A How-to Manual. Oxford. Oxford University Press.
Hashim, A. M., Alatawi, A., Altaf, F. M., Qari, S. H., Elhady, M. E., Osman, G. H., & Abouseadaa, H. H. (2021). Phylogenetic relationships and DNA barcoding of nine endangered medicinal plant species endemic to Saint Katherine protectorate. Saudi Journal of Biological Sciences, 28(3), 1919–1930.
Letsiou, S., Madesis, P., Vasdekis, E., Montemurro, C., Grigoriou, M. E., Skavdis, G., Moussis, V., Koutelidakis, A. E., & Tzakos, A. G. (2024). DNA Barcoding as a Plant Identification Method. Applied Sciences (Switzerland), 14(4), 1–12.
Rahayu, D. A., & Jannah, M. (2022). Dna Barcode Hewan Dan Tumbuhan Indonesia. 9–25.
Sagala, L.R., Lazuardi, Harahap., F., Manalu, K., Idami, Z., & Prasetya, E. (2021). DNA Barcoding Zingiber loerzingii Valeton menggunakan lokus gen Ribulose-1,5- biphosphate Carboxylase-OxygenaseLarge subunit Gene (rbcL). Jurnal Biosains, 7(3), 166-173.
Tamura, K., Stecher, G., & Kumar, S. 2021. MEGA11: Molecular Evolutionary Genetic Analysis Version 11. Molecular Biology and Evolution, 38(7), 3022-3027.
Wang, D-Y., Wang, Q., Wang, Y-L., Xiang, X-G., Huang, L-Q., and Jin, X-H. (2017). Evaluation of DNA Barcodes in Codonopsis (Campanulaceae) and in some Large Angiosperm Plant Genera. PLoS ONE, 12(2), 1-14..
Xu, J., Zang, F., Wu, Q., Wang, Y., Wang, B., Huang, P., Zang, D., Ma, Y., & Zheng, Y. (2021). Analysis of the genetic diversity and molecular phylogeography of the endangered wild rose (Rosa rugosa) in China based on chloroplast genes. Global Ecology and Conservation, 28, 1-12.
Yan, M., Dong, S., Gong, Q., Xu, Q., & Ge, Y. (2023). Comparative chloroplast genome analysis of four Polygonatum species insights into DNA barcoding, evolution, and phylogeny. Scientific Reports, 13(1), 1–23.
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Published
2024-12-30
How to Cite
Idami, Z. . (2024). IDENTIFIKASI DNA BARCODING TUMBUHAN BALAKKA (Phyllanthus emblica L.) BERDASARKAN LOKUS GEN rbcL. Jurnal Biogenerasi, 10(1), 541–546. https://doi.org/10.30605/biogenerasi.v10i1.5145
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