ANALISIS MOLEKULER KERANG (Anadara sp.) MENGGUNAKAN MARKER GEN CYTOCHROME OXYDASE SUBUNIT 1 (CO1) DI PERAIRAN PERCUT SEI TUAN

Authors

  • Anggrainy Dwi Pratiwi
  • Zahratul Idami Universitas Islam Negeri Sumatera Utara
  • Kartika Manalu Universitas Islam Negeri Sumatera Utara

DOI:

https://doi.org/10.30605/biogenerasi.v10i1.4835

Keywords:

Anadara sp, CO1 Gene, Phylogenetic Tree

Abstract

Anadara sp. clams are very popular clams among the community. Anadara sp. also usually live in waters with muddy and sandy surfaces. DNA barcoding is a barcode sequence to identify unknown species. CO1 is a molecular marker commonly used in molecular research on animals. The purpose of this study was to determine the species of Anadara sp. clams found in Percut Sei Tuan Waters using gene markers, the diversity of Anadara sp. clams using CO1 Gene Markers, and the kinship of Anadara sp. clams found in Percut Sei Tuan Waters. This study has 4 stages, namely DNA isolation using the Favorgen kit, PCR amplification, electrophoresis and sequencing. The DNA sequencing results were analyzed using the MEGA 11 application. The results showed that of the three samples that had been analyzed using the CO1 gene marker obtained using BLAST and NCBI, namely the Anadara granosa, Anadara antiquata, and Anadara gubernaculum samples. With the calculation results of higher AT content (28.9%) and lower GC content (20.12%) which states that the species is more primitive. In the pairwise distance matrix, the samples of Anadara antiquata (Percut Sei Tuan) and Anadara granosa (Percut Sei Tuan) which are in the same clade with different sub clades have a genetic distance ranging from 111.45 to 213.77. The phylogenetic tree also shows that the three samples have a close kinship relationship.

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References

Ginting, E.D.D., Susetya, I.E., Pratana, P., & Desrita. 2017. Identifikasi Jenis-Jenis Bivalvia di Perairan Tanjungbalai, Provinsi Sumatera Utara. Acta Aquatica. 4 (1). 13-20.
Ginting, T.V.B., Nasution, S., & Effendi, I. 2021. Perubahan Genetik pada Kerang Sipetang (Pharella acutidens) yang Dipengaruhi oleh Logam Pb dan Cd di Perairan Selat Rupat. Jurnal Natur Indonesia. 19 (2). 43-50.
Khaira, K., Tambunan, E.P.S., & Idami, Z. 2024. DNA Barcoding Pada Tumbuhan Harimonting (Rhodomyrtus tomentosa) Menggunakan Gen trnL-F. Best Journal Biology Education Science & Technology. 7(1). 366-372.
Monalisa, E., Mantiri, F.R., & Lengkong, H.J. 2019. Kajian Variasi Sekuens Interspesies dan Filogeni Kelelawar Pteropus sp. Menggunakan Gen CO1. Jurnal MIPA Unsrat. 8 (2). 71-77.
Rahayu, W.P., Rinanti, E., Nurjanah, S., & Nurwitri, C.C. 2016. Identifikasi Liseria monocytogenes Pada Kerang Hijau dan Kerang Darah. JPHPI. 19 (3). 329-338.
Saleky, D. & Merly, S.L. 2021. Pendekatan DNA Barcoding Untuk Identifikasi Cassidula angulifera (Petit, 1841) (Moluska : Gastropoda). Jurnal Sumberdaya Akuatik Indopasifik. 5 (1). 55-64.
Simamora, H.P.M & Tumanggor, L. 2022. Analilis Kadar Timbal (Pb) Pada Kerang (Anadara sp) yang Di Jual Di Pasar Ikan Belawan dan Pasar Ikan Percut Sei Tuan. Nutrition And Life Journal. 1 (1). 1-7.
Zain, M.S.A. & Sulandari, S. 2008. Keragaman Genetik Ayam Lombok Berdasarkan Sekuen D-LOOP DNA Mitokondria. JITV. 13 (4). 308-314.

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Published

2024-11-15

How to Cite

Dwi Pratiwi, A. ., Idami, Z. ., & Manalu, K. . (2024). ANALISIS MOLEKULER KERANG (Anadara sp.) MENGGUNAKAN MARKER GEN CYTOCHROME OXYDASE SUBUNIT 1 (CO1) DI PERAIRAN PERCUT SEI TUAN. Jurnal Biogenerasi, 10(1), 458–463. https://doi.org/10.30605/biogenerasi.v10i1.4835

Issue

Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025

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