Formulasi dan Uji Efektivitas Sediaan Sirup Ekstrak Daun Kacapiring (Gardenia jasminoides J. Ellis) Sebagai Antipiretik Terhadap Mencit (Mus musculus L) YANG DI INDUKSI VAKSIN DPT
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Abstract
Flavonoids in plate glass leaves (Gardenia jasminoides J. Ellis) can inhibit the cyclooxygenase enzyme, especially cyclooxygenase-2, which plays a role in prostaglandin biosynthesis so that the process of fever is inhibited. In addition, flavonoids and paracetamol have a similar structure. The aim of this research was to test the syrup extract of G. jasminoides leaves to have antipyretic effectiveness against male mice (Mus musculus) induced by DPT vaccine. The research method used was experimental research. The research stages were plant determination, the manufacture of G. jasminoides leaf extract using the maceration method with 96% ethanol solvent, phytochemical screening, syrup formulation with the active substance of G. jasminoides leaf extract and additives in the form of saccharose, citric acid, grape essence, propylene glycol and distilled water. Antipyretic testing was carried out in vivo, namely the male mice were measured at the initial oral temperature, then injected intramuscularly with the DPT HB vaccine in the thigh, two hours after experiencing optimum fever (above normal temperature), then each group was treated with oral method, namely negative control (syrup basis), positive control (paracetamol syrup), ethanol extract syrup from G. jasminoides leaves dose 1; 3 and 5 mg / 20gBW. The results showed that extract G. jasminoides could be formulated into syrup preparations with good physical quality in terms of organoleptic, density, volume transferred and pH. The dose of G. jasminoides leaf extract syrup 5 mg/20Gbb was the most effective dose as an antipyretic in male mice induced with DPT vaccine with a total temperature reduction of 2oC.
Keywords: antipyretic, gardenia, syrup.
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References
Diniatik. 2015. Penentuan Kadar Flavonoid Total Ekstrak Etanolik Daun Kepel (Stelechocarpus burahol (Bl.) Hook f. & Th.) Dengan Metode Spektrofotometri. Kartika-Jurnal Ilmiah Farmasi, Jun 2015, 3 (1), 1-5.
Fadhil, M., Desnita, E & Elianora, D. 2017. Uji Efektifitas Ekstrak Biji Mahoni (Swietenia Mahagoni (L.) Jacq) Sebagai Antipiretik Pada Tikus Wistar (Rattus norvegicus). Jurnal B-Dent. 4(2) : 141 – 149.
Jansen, I., J. Wuisan & H.Awaloei. 2015. Uji Efek Antipiretik Ekstrak Meniran (Phyllantus niruri L.) Pada Tikus Wistar (Rattus norvegicus) Jantan Yang Diinduksi Vaksin DPT-HB. Jurnal e-Biomedik (eBm), 3(1): 470-474.
Kalay, S.,Bodhi, W., dan Yamlean, P.V., 2014. Uji Efek Antipiretik Ekstrak Etanol Daun Prasman (Eupatorium triplinerve Vahl) Pada Tikus Jantan Galur Wistar (Rattus norvegicus L) Yang Diinduksi Vaksin DPT HB. Program Studi Farmasi Universitas Sam Ratulangi. Manado.
Malik, Andryani Ningsi, Mentarry Bafadal, Dwi Nur Saktiani & Wahyuni. 2018. Uji Efek Antipiretik Ekstrak Etanol Buah Wualae (Etlingera elatior (Jack) R.M. Smith) Terhadap Mencit Jantan (Mus musculus L.) Galur Balb/C. Pharmauho Volume 4, No. 1, April 2018,Hal. 9-11.
Nadi, I Wayan Surudarma, Desak Made Wihandani, I Wayan Gede Sutadarma & Agung Nova Mahendra. 2021. Perbandingan Selisih Kadar Glukosa Darah Puasa Dan Dua Jam Post-Prandial Terhadap Pemberian Nasi Beras Putih, Nasi Beras Merah, Dan Nasi Beras Hitam Pada Mencit Jantan (Mus musculus L.) Galur Swiss webster. Jurnal Medika Udayana. 10 (2).
Nuralifah, Fery Indradewi Armadani & Ni Nyoman Fitri Astari. 2019. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kacapiring (Gardenia jasminoides Ellis) Terhadap Bacteri Staphylococcus aureus dan Propionibacterium acnes. Medula , Voume 6, Suplemen: 617-628.
Purwati, Sonja V. T. Lumowa & Samsurianto. 2017. Skrining Fitokimia Daun Saliara (Lantana camara L) Sebagai Pestisida Nabati Penekan Hama Dan Insidensi Penyakit Pada Tanaman Holtikultura Di Kalimantan Timur. Prosiding Seminar Nasional Kimia 2017. Kimia FMIPA Universitas Mulawarman.
Simaremare, E.S. 2014. Skrining Fitokimia Ekstrak Etanol Daun Gatal (Laportea decumana (Roxb.) Wedd). PHARMACY, Vol.11 No. 01: 98-107.
Supomo, R. Supriningrum & R. Junaid. 2016. Karakterisasi Dan Skrining Fitokimia Daun Kerehau (Callicarpa longifolia Lamk.). Jurnal Kimia Mulawarman. 13(2). 89-96.
Tawi, Wilmar Maarisit, Olvie S. Datu & Yessie K. Lengkey. 2019. Uji Efektivitas Ekstrak Etanol Daun Awar-awar Ficus septica Burm F. Sebagai Antipiretik Terhadap Tikus Putih Rattus novergicus. Jurnal Biofarmasetikal Tropis. 2 (1), 1-9.
World Health Organization. 2014. 10 facts on immunization. Diakses dari http://www.who.int/en/news-room/fact-