LITERATUR REVIEW: APLIKASI MOLEKULER BERBASIS PCR DALAM DETEKSI  DAN KARAKTERISASI Escherichia coli PADA SEKTOR LINGKUNGAN, PANGAN, DAN KLINIS

Authors

  • Diva Yunavita Universitas Negri Padang
  • Dwi Hilda Putri State University of Padang image/svg+xml

DOI:

https://doi.org/10.30605/jcp1q263

Keywords:

Quality of service for cadres, toddlers, maternal visits

Abstract

Escherichia coli is a gram-negative bacterium and a key indicator of water and food contamination. Conventional PCR is a widely used molecular method for specific, rapid, and sensitive E. coli detection. This literature review systematically examines the application of conventional PCR in E. coli detection across water, food, and clinical sample matrices from 2017 to 2025. Thirteen scientific articles were reviewed regarding research objects, PCR methods, research stages, and findings. Conventional PCR using primers 16E1/16E2 (584 bp), Afa FP/RP (480 bp), and ESS (825 bp) effectively detected E. coli in diverse samples. PCR showed superior sensitivity (100%) and specificity (94.34%) compared to culture methods (Hariri, 2022). Direct PCR offered a cost-effective alternative without DNA extraction (JKG, 2025). Multiplex PCR enabled simultaneous detection of multiple virulence genes (Ekawati et al.; Microbiology Spectrum, 2024). Conventional PCR remains a reliable, specific, and rapid tool for E. coli detection across diverse sample types.

References

Arai, et al. (2025). An Optimized PCR Assay to Detect E. coli Harboring the astA Gene (EAST1) in Various Food Matrices. Food Safety (FSCJ). DOI: 10.14252/foodsafetyfscj.D-25-00013

Bioma: Jurnal Biologi Makassar. (2021). Deteksi Molekuler Bakteri Escherichia coli sebagai Penyebab Penyakit Diare dengan Menggunakan Teknik PCR. journal.unhas.ac.id/index.php/bioma/article/view/13194

CIHAMS: Conference on Innovation in Health, Accounting and Management Sciences. (2020). Deteksi Escherichia coli dengan Metode Polimerase Chain Reaction (PCR): Literature Review. cihams.setiabudi.ac.id/index.php/proceeding/article/view/24

Ekawati, et al. Deteksi Escherichia coli Patogen pada Pangan Menggunakan Metode Konvensional dan Multiplex PCR. Jurnal SainHealth, 1(2), 23–31.

Hariri, S. (2022). Detection of Escherichia coli in Food Samples Using Culture and Polymerase Chain Reaction Methods. Cureus, 14(12): e32808. DOI: 10.7759/cureus.32808

JIFI: Jurnal Ilmiah Farmasi Imelda. (2023). Identifikasi Bakteri Escherichia coli pada Saus Makanan Jajanan Cilok di Sekolah Dasar Kecamatan Indralaya Selatan. 7(1). jurnal.uimedan.ac.id/index.php/JURNALFARMASI/article/view/1505

Jurnal Kesmas dan Gizi (JKG). (2025). Direct PCR for Escherichia coli: A Straightforward and Cost-Effective Method. Mediastra. ejournal.medistra.ac.id/index.php/JKG/article/view/2606

Jurnal Wiyata: Penelitian Sains dan Kesehatan. (2025). Desain Primer dan Pemurnian Ganda untuk Deteksi PCR Gen 16S rRNA pada Escherichia coli. IIK Kediri. ojs.iik.ac.id/index.php/wiyata/article/view/1007

Makara Sains, 14(1): 39–43. Deteksi Cepat Bakteri Escherichia coli dalam Sampel Air dengan Metode PCR Menggunakan Primer 16E1 dan 16E2. researchgate.net/publication/47416691

Microbiology Spectrum / PMC. (2024). Novel Multiplex-PCR Test for Escherichia coli Detection Using Genes cydA, lacY, and ydiV. DOI: 10.1128/spectrum.03773-23. PMC11237426

PMC / Cureus. (2025). Detection of Virulence Determinants of Uropathogenic Escherichia coli (UPEC). PMC11923561

Putri, et al. (2022). Deteksi Bakteri Escherichia coli dengan Metode PCR pada Sampel Makanan Takjil. Prosiding Semnas Biologi FMIPA UNP, 2(1), 405–413. DOI: 10.24036/prosemnasbio/vol2/404

Putri & Achyar. (2024). Optimasi Isolasi DNA Bakteri Patogen (E. coli) pada Sampel Air Sungai Berbasis PCR. Jurnal Serambi Biologi, UNP. serambibiologi.ppj.unp.ac.id/index.php/srmb/article/view/241

Downloads

Published

2026-05-11

Issue

Vol. 11 No. 2 (2026): April - Juni 2026

Section

Articles

License

Copyright (c) 2026 Diva Yunavita, Dwi Hilda Putri

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

In submitting the manuscript to the journal, the authors certify that:

  • They are authorized by their co-authors to enter into these arrangements.
  • The work described has not been formally published before, except in the form of an abstract or as part of a published lecture, review, thesis, or overlay journal.
  • That it is not under consideration for publication elsewhere,
  • That its publication has been approved by all the author(s) and by the responsible authorities – tacitly or explicitly – of the institutes where the work has been carried out.
  • They secure the right to reproduce any material that has already been published or copyrighted elsewhere.
  • They agree to the following license and copyright agreement.

License and Copyright Agreement

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

How to Cite

LITERATUR REVIEW: APLIKASI MOLEKULER BERBASIS PCR DALAM DETEKSI  DAN KARAKTERISASI Escherichia coli PADA SEKTOR LINGKUNGAN, PANGAN, DAN KLINIS. (2026). Jurnal Biogenerasi, 11(2), 717-722. https://doi.org/10.30605/jcp1q263