Literature Review: Bakteri Asam Laktat Dari Dadih Minangkabau: Keragaman, Karakteristik, Dan Potensi Aktivitas Antibakteri
DOI:
https://doi.org/10.30605/wmgnc484Keywords:
Aktivitas Antibakteri, Bakteri Asam Laktat, Dadih, Fermentasi, ProbiotikAbstract
Dadih merupakan produk susu fermentasi tradisional khas Minangkabau, Sumatera Barat, yang diproduksi melalui fermentasi spontan susu kerbau dalam tabung bambu. Produk ini telah lama dikenal sebagai sumber bakteri asam laktat (BAL) yang kaya dan beragam, dengan potensi manfaat sebagai agen probiotik, antibakteri, antioksidan, dan biopreservatif alami. Artikel review ini mengintegrasikan temuan dari berbagai studi ilmiah mengenai keragaman spesies BAL yang diisolasi dari dadih, karakteristik morfologi dan biokimianya, serta potensi aktivitas antibakterinya terhadap bakteri patogen pangan, terutama Escherichia coli dan Staphylococcus aureus. Kajian literatur menunjukkan dominasi genus Lactobacillus, Lactococcus, Pediococcus, Enterococcus, Weissella, dan Leuconostoc dalam komunitas BAL dadih. Aktivitas antibakteri BAL dadih diperantarai oleh produksi senyawa bioaktif seperti asam laktat, hidrogen peroksida, diasetil, dan bakteriosin, dengan mekanisme kerja yang melibatkan permeabilisasi membran sel dan gangguan sintesis dinding sel patogen. Isolat BAL dari dadih secara konsisten menunjukkan penghambatan lebih kuat terhadap bakteri Gram-positif (S. aureus) dibandingkan Gram-negatif (E. coli), yang dikaitkan dengan perbedaan struktur dinding sel. Dadih Minangkabau berpotensi besar sebagai sumber BAL unggulan untuk pengembangan probiotik dan biopreservatif pangan berbasis kearifan lokal
References
Arnold, M., Rajagukguk, Y. V., & Gramza-Michałowska, A. (2021). Characterization of dadih: Traditional fermented buffalo milk of minangkabau. Beverages, 7(3). https://doi.org/10.3390/beverages7030060
Daffa Sonik, M., Neldi, V., & Ramadhani, P. (2023). REVIEW ARTIKEL: EFEKTIVITAS DADIH (YOGURT KHAS SUMATRA BARAT) SEBAGAI PROBIOTIK. Jurnal Farmasi Higea, 15(1). www.jurnalfarmasihigea.org
El Ahmadi, K., Haboubi, K., El Allaoui, H., El Hammoudani, Y., Bouhrim, M., Eto, B., Shahat, A. A., & Herqash, R. N. (2025). Isolation and preliminary screening of lactic acid bacteria for antimicrobial potential from raw milk. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1565016
Fagheei Aghmiyuni, Z., Saderi, H., Owlia, P., & Saidi, N. (2024). Evaluation of the Effect of Lactobacillus acidophilus ATCC 4356 Bacteriocin against Staphylococcus aureus. BioMed Research International, 2024. https://doi.org/10.1155/2024/4119960
Goa, T., Beyene, G., Mekonnen, M., & Gorems, K. (2022). Isolation and Characterization of Lactic Acid Bacteria from Fermented Milk Produced in Jimma Town, Southwest Ethiopia, and Evaluation of their Antimicrobial Activity against Selected Pathogenic Bacteria. International Journal of Food Science, 2022. https://doi.org/10.1155/2022/2076021
Harnentis, H., Marlida, Y., Nur, Y. S., Wizna, W., Santi, M. A., Septiani, N., Adzitey, F., & Huda, N. (2020). Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia. Veterinary World, 13(9), 1922–1927. https://doi.org/10.14202/vetworld.2020.1922-1927
Herlina, V. T., & Setiarto, R. H. B. (2024). From tradition to innovation: dadih, the Minangkabau tribe’s traditional fermented buffalo milk from Indonesia. Journal of Ethnic Foods, 11(1). https://doi.org/10.1186/s42779-024-00234-6
Ismael, M., Gu, Y., Cui, Y., Wang, T., Yue, F., Qin, Y., & Lü, X. (2022). Probiotic of Lactiplantibacillus plantarum NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation. Foods, 11(23). https://doi.org/10.3390/foods11233766
Kalhoro, M. S., Anal, A. K., Kalhoro, D. H., Hussain, T., Murtaza, G., & Mangi, M. H. (2023). Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk. Oxidative Medicine and Cellular Longevity, 2023. https://doi.org/10.1155/2023/8475995
Kolech, A., Alemu, S., & Milkessa, T. (2026). Evaluation of the antimicrobial properties of potential probiotic lactic acid bacteria from raw and fermented cow milk. Scientific Reports, 16(1). https://doi.org/10.1038/s41598-026-39586-1
Lei, S., Zhao, R., Sun, J., Ran, J., Ruan, X., & Zhu, Y. (2020). Partial purification and characterization of a broad-spectrum bacteriocin produced by a Lactobacillus plantarum zrx03 isolated from infant’s feces. Food Science and Nutrition, 8(5), 2214–2222. https://doi.org/10.1002/fsn3.1428
Mulyadi, V. F., Wesha, N. S. P., Yandi, S., & Ningrum, V. (2020). Efektivitas Topikal Aplikasi Dadih terhadap Inflamasi Gingiva. Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva, 9(1). https://doi.org/10.18196/di.9110
Phan, A., Mijar, S., Harvey, C., & Biswas, D. (2025). Staphylococcus aureus in Foodborne Diseases and Alternative Intervention Strategies to Overcome Antibiotic Resistance by Using Natural Antimicrobials. Microorganisms, 13(8). https://doi.org/10.3390/microorganisms13081732
Sonik, M. D., Neldi, V., & Ramadhani, P. (2023). Review Artikel: Efektivitas Dadih (Yogurt Khas Sumatra Barat) Sebagai Probiotik. Jurnal Farmasi Higea, 15(1), 77. https://doi.org/10.52689/higea.v15i1.542
Sperry, M. F., Silva, H. L. A., Balthazar, C. F., Esmerino, E. A., Verruck, S., Prudencio, E. S., Neto, R. P. C., Tavares, M. I. B., Peixoto, J. C., Nazzaro, F., Rocha, R. S., Moraes, J., Gomes, A. S. G., Raices, R. S. L., Silva, M. C., Granato, D., Pimentel, T. C., Freitas, M. Q., & Cruz, A. G. (2018). Probiotic Minas Frescal cheese added with L. casei 01: Physicochemical and bioactivity characterization and effects on hematological/biochemical parameters of hypertensive overweighted women – A randomized double-blind pilot trial. Journal of Functional Foods, 45, 435–443. https://doi.org/10.1016/j.jff.2018.04.015
Veettil, V. N., & Chitra, A. V. (2022). Optimization of bacteriocin production by Lactobacillus plantarum using Response Surface Methodology. Cellular and Molecular Biology, 68(6), 105–110. https://doi.org/10.14715/cmb/2022.68.6.17
Venema, K., & Surono, I. S. (2019). Microbiota composition of dadih – a traditional fermented buffalo milk of West Sumatra. Letters in Applied Microbiology, 68(3), 234–240. https://doi.org/10.1111/lam.13107
Wang, P., Wei, Y., Yertai, Y., Li, W., & Mamet, T. (2025). The physicochemical and nutritional profile of high protein yak milk powder. International Dairy Journal, 165. https://doi.org/10.1016/j.idairyj.2025.106195
Wirawati, C. U., Sudarwanto, M. B., Lukman, D. W., Wientarsih, I., & Srihanto, E. A. (2019). Diversity of lactic acid bacteria in dadih produced by either back-slopping or spontaneous fermentation from two different regions of West Sumatra, Indonesia. Veterinary World, 12(6), 823–829. https://doi.org/10.14202/vetworld.2019.823-829
Yuliana, T., Tyano, F. N., Harlina, P. W., Cahyana, Y., Marta, H., & Krama, A. (2023). Characterizing Probiotic Lactic Acid Bacteria from Buffalo Milk Fermentation (Dadih) for Beef Biopreservation. Applied Sciences (Switzerland), 13(24). https://doi.org/10.3390/app132413089
ZAENAL MUSTOPA, A., & FATIMAH, F. (2014). Diversity of Lactic Acid Bacteria Isolated from Indonesian Traditional Fermented Foods. Microbiology Indonesia, 8(2), 48–57. https://doi.org/10.5454/mi.8.2.2
Zapaśnik, A., Sokołowska, B., & Bryła, M. (2022). Role of Lactic Acid Bacteria in Food Preservation and Safety. Foods, 11(9). https://doi.org/10.3390/foods11091283
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Rahmi Adzra Haura, Dandy Yusuf, Dwi Hilda Putri

This work is licensed under a Creative Commons Attribution 4.0 International License.
In submitting the manuscript to the journal, the authors certify that:
- They are authorized by their co-authors to enter into these arrangements.
- The work described has not been formally published before, except in the form of an abstract or as part of a published lecture, review, thesis, or overlay journal.
- That it is not under consideration for publication elsewhere,
- That its publication has been approved by all the author(s) and by the responsible authorities – tacitly or explicitly – of the institutes where the work has been carried out.
- They secure the right to reproduce any material that has already been published or copyrighted elsewhere.
- They agree to the following license and copyright agreement.
License and Copyright Agreement
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
.png)
