Efek Seed Priming KNO3 terhadap Perkecambahan dan Pertumbuhan Awal Benih Padi Varietas IR 64 pada Beberapa Tingkat Salinitas

Authors

DOI:

https://doi.org/10.30605/hcqyq463

Keywords:

Seed priming, KNO3, salinity, IR 64 rice, germination

Abstract

Salinity is one of the major limiting factors in rice cultivation, negatively affecting seed germination and early plant growth. One approach that can be taken to improve seed tolerance under saline conditions is seed priming. This study aims to examine the effect of potassium nitrate (KNO3) seed priming on the germination and early growth of IR 64 rice seeds at various salinity levels. This study was an experiment designed using a completely randomized design with a single treatment factor, namely NaCl salinity levels (0, 50, 100, and 150 mM), in which all seeds were treated with 1% KNO3 seed priming prior to stress application. Each treatment was replicated four times. The observed parameters included germination percentage, root length, shoot length, seedling length, and seed vigor index. The data were analyzed using analysis of variance (ANOVA) followed by Duncan’s multiple range test at a 5% significance level. The results showed that KNO3 seed priming was able to maintain the germination percentage of IR 64 seeds at all salinity levels, with values that were not statistically significantly different. However, increasing NaCl concentrations had a significant negative effect on the morphological growth of seedlings, as indicated by a decrease in root length, shoot length, seedling length, and seed vigor index. The treatment without salinity produced the best early growth, while high salinity significantly inhibited seedling growth. Based on these results, it can be concluded that KNO3 seed priming plays an effective role in maintaining the viability of IR 64 rice seeds under saline conditions, but it is not yet fully capable of overcoming the negative effects of salinity on early plant growth.

References

Abdul‐Baki, A. A., & Anderson, J. D. (1973). Vigor determination in soybean seed by multiple criteria. Crop Science, 13(6), 630-633.

Ali, L. G., Nulit, R., Ibrahim, M. H., & Yien, C. Y. S. (2021). Efficacy of KNO3, SiO2 and SA priming for improving emergence, seedling growth and antioxidant enzymes of rice (Oryza sativa), under drought. Scientific reports, 11(1), 3864.

Coca, L. I. R., García González, M. T., Gil Unday, Z., Jiménez Hernández, J., Rodríguez Jáuregui, M. M., & Fernández Cancio, Y. (2023). Effects of sodium salinity on rice (Oryza sativa L.) cultivation: A review. Sustainability, 15(3), 1804.

Guan, R. X., Guo, X. Y., Qu, Y., Zhang, Z. W., Bao, L. G., Ye, R. Y., Chang, R. Z., & Qiu, L. J. (2024). Salt tolerance in soybeans: Focus on screening methods and genetics. Plants, 13(1), 97.

Hakim, M. A., Juraimi, A. S., Begum, M., Hanafi, M. M., Ismail, M. R., & Selamat, A. (2010). Effect of salt stress on germination and early seedling growth of rice (Oryza sativa L.). African journal of biotechnology, 9(13), 1911-1918.

Janata, B. S., Grover, S., Ram, H., & Baath, G. S. (2024). Seed priming: Molecular and physiological mechanisms underlying biotic and abiotic stress tolerance. Agronomy, 14(12), 2901.

MacDonald, M. T., & Mohan, V. R. (2025). Chemical seed priming: Molecules and mechanisms for enhancing plant germination, growth, and stress tolerance. Current Issues in Molecular Biology, 47(3), 177.

Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annu. Rev. Plant Biol., 59(1), 651-681.

Pharmawati, M., & Wijaya, I. M. A. S. (2019). Changes in growth, biochemical components and antioxidant genes expression in rice seedling (Oryza sativa L.) cultivar ‘IR 64’ under salt stress. Indian Journal of Agricultural Research, 53(4), 478-482.

Purwestri, Y. A., Nurbaiti, S., Putri, S. P. M., Wahyuni, I. M., Yulyani, S. R., Sebastian, A., Nuringtyas, T. R., & Yamaguchi, N. (2023). Seed halopriming: A promising strategy to induce salt tolerance in Indonesian pigmented rice. Plants, 12(15), 2879.

Rathinavel, K., Priyadharshini, C., & Kavitha, H. (2020). Effect of potassium nitrate (KNO3) osmopriming on seed germination and seedling vigour in cotton (Gossypium hirsutum L.). International Journal of Agriculture, Environment and Research (IJAER), 6(4), 694-702.

Rhaman, M. S., Imran, S., Rauf, F., Khatun, M., Baskin, C. C., Murata, Y., & Hasanuzzaman, M. (2020). Seed priming with phytohormones: An effective approach for the mitigation of abiotic stress. Plants, 10(1), 37.

Rehman, M. M. U., Liu, J., Nijabat, A., Alsudays, I. M., Saleh, M. A., Alamer, K. H., Attia, H., Ziaf, K., Zaman, Q. U., & Amjad, M. (2024). Seed priming with potassium nitrate alleviates the high temperature stress by modulating growth and antioxidant potential in carrot seeds and seedlings. BMC Plant Biology, 24(606).

Sackey, O. K., Feng, N., Mohammed, Y. Z., Dzou, C. F., Zheng, D., Zhao, L., & Shen, X. (2025). A comprehensive review on rice responses and tolerance to salt stress. Frontiers in Plant Science, 16, 1561280.

Talavera-Mateo, L., García, A., & Santamaría, M. E. (2023). A comprehensive meta-analysis reveals the key variables and scope of seed defense priming. Frontiers in Plant Science, 14, 1208449.

Taratima, W., Chomarsa, T., & Maneerattanarungroj, P. (2022). Salinity stress response of rice (Oryza sativa L. cv. Luem Pua) calli and seedlings. Scientifica, 2022(1), 5616683.

Theerakulpisut, P., Kanawapee, N., & Panwong, B. (2017). Seed priming alleviated salt stress effects on rice seedlings by improving Na+/K+ balance and maintaining membrane integrity. Plant Biology, 7(1), 39-46.

Yadav, C., Bahuguna, R. N., Dhankher, O. P., Singla-Pareek, S. L., & Pareek, A. (2022). Physiological and molecular signatures reveal differential response of rice genotypes to drought and drought combination with heat and salinity stress. Physiology and Molecular Biology of Plants, 28(4), 899-910.

Downloads

Published

2026-04-29

How to Cite

Efek Seed Priming KNO3 terhadap Perkecambahan dan Pertumbuhan Awal Benih Padi Varietas IR 64 pada Beberapa Tingkat Salinitas. (2026). Jurnal Biogenerasi, 11(2), 660-665. https://doi.org/10.30605/hcqyq463