TINGKAT KONTAMINASI ANGGREK Vanda limbata DENGAN EKSTRAK BAHAN ALAMI YANG BERBEDA SECARA IN VITRO
DOI:
https://doi.org/10.30605/biogenerasi.v10i1.4942Keywords:
Vanda limbata, organic, contaminationAbstract
The aim of this research to determine the lowest percentage of contamination and growth of Vanda limbata orchids with the addition of organic media extract. This research was carried out from July to September 2022 at the Biology Education Laboratory, Faculty of Teacher Training and Education, University of Timor. This study used a randomized block design with 3 natural media treatments (tomato extract, bean sprouts and Ambon banana) with 5 replications. From this research, it can be shown that natural-based media with tomato extract showed the lowest percentage of contamination, namely 0%. With the addition of tomato extract, it was able to stimulate the growth of Protocorm Likes Bodies (PLB) at the age of 27 days after planting compared to organic media with the addition of bean sprout extract and media. organic with the addition of Ambon banana extract and the highest percentage of contamination was found in organic media with the addition of Ambon banana extract, namely 16%.
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References
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Yusuf, Y., & Indiarto, A. (2015). Pertumbuhan Dan Perkembangan Embrio Anggrek Vanda Limbata Blume X Vanda Tricolor Lindl . Pada Medium Pupuk Organik Cair Secara In Vitro. Prosiding Seminar Nasional Biologi Dan Pembelajarannya, 533–538.
Agustin, R., Suharsono, S., & Putra, R. R. (2020). Pengaruh Ekstrak Tomat Terhadap Pertumbuhan Embrio Anggrek Phaius Tankervilleae Khas Gunung Galunggung Kabupaten Tasikmalaya. Bioma : Jurnal Ilmiah Biologi, 9(2), 264–279. Https://Doi.Org/10.26877/Bioma.V9i2.7064
Az-Zahra, N. U. R. F. (2020). Analisis Keanekaragaman tanaman anggrek Danau Ranamese Taman Wisata Alam Ruteng Nusa Tenggara Timur. Muhammadiyah Malang.
Clarissa, O., & Halim, M. (2019). Taman Wisata Dan Konservasi Anggrek Nusantara. Jurnal Sains, Teknologi, Urban, Perancangan, Arsitektur (Stupa), 1(1), 408. Https://Doi.Org/10.24912/Stupa.V1i1.3966
Corina, I. P., Mukarlina, & Linda, R. (2014). Respon Pertumbuhan Kultur Biji Jeruk Siam Seed ( Citrus Nobilis Var . Microcarpa ) Dengan Penambahan Ekstrak Tauge Dan Benzilaminopurine ( Bap ). Protobiont, 3(2), 120–124.
Dwiyani, R., Yuswanti, H., Darmawati, I. A. P., Suada, K., & Mayadewi, N. N. A. (2015). In Vitro Germination And Its Subsequent Growth Of An Orchid Of Vanda Tricolor Lindl. Var. Suavis From Bali On Complex Additives Enriched Medium. Agrivita, 37(2), 144–150. Https://Doi.Org/10.17503/Agrivita-2015-37-2-P144-150
Febriandito, P. D., & Soetopo, L. (2019). Eksplorasi Keanekaragaman Anggrek Epifit Di Kawasan Konservasi Wilayah Ii Senduro , Blok Ireng-Ireng , Taman Nasional Bromo Tengger Semeru , Jawa Timur. Plantropica Journal Of Agricultural Science, 4(1), 68–76.
Handini, E., & Puspitaningtyas, Dwi Murti. (2020). Perkecambahan Dan Prediksi Masa Hidup Tiga Jenis Dendrobium Menggunakan Empat Macam Formulasi Media. Buletin Kebun Raya, 23(3), 187–195.
Hapsoro, D., & Yusnita. (2018). Kultur Jaringan: Teori Dan Praktik (A. Pramesta, D. Nofiyanto, A. K, & B. Belinda (Eds.); Andi (Angg). Cv Andi Offset.
Heriansyah, P., & Indrawanis, E. (2020). Uji Tingkat Kontaminasi Eksplan Anggrek Bromheadia Finlysoniana L.Miq Dalam Kultur In Vitro Dengan Penambahan Ekstrak Tomat. Agroqua, 18(2), 223–232. Https://Doi.Org/10.32663/Ja.V
Lasa, S. C., Purnama, M. M. E., & Pellondo’u, M. E. (2021). Identifikasi Keanekaragaman Jenis - Jenis Anggrek ( Orchidaceae ) Di Hutan Lindung Desa Timur. Wana Lestari, 04(01), 082–091.
Nurbaiti, R. (2016). Studi Pengecambahan Biji Dan Pertumbuhan Seedling Anggrek Dendrobium Hibrida In Vitro : Pengaruh Media Dasar, Ekstrak Tomat Dan Arang Aktif. Lampung.
Nurfadilah, Mukarlina, & P.W, E. R. (2018). Multiplikasi Anggrek Hitam ( Coelogyne Pandurata Lindl ) Pada Media Murashige Skoog ( Ms ) Dengan Penambahan Ekstrak Pisang Ambon Dan Benzyl Amino Purin ( Bap ). Protobiont, 7(3), 47–53.
Nurfadilah, S. (2016). Pengaruh Media Kultur Dan Arang Aktif Pada Perkecambahan Biji Dan Perkembangan Seddling Anggrek Langka Dendrobium Taurulinum J.J. Smith In Vitro. Ilmu-Ilmu Hayati, 15(1), 49–57.
Rahayu, E. M. Della, & Mulyani, M. (2020). Asymbiotic Seed Germination And Plantlet Development Of Dendrobium Spectabile ( Blume ) Miq . Buletin Kebun Raya, 23(April), 25–35.
Rahmadi, A., Wicaksana, N., Nurhadi, B., Suminar, E., & Mubarok, S. (2020). Optimasi Teknik Sterilisasi Dan Induksi Tunas Tanaman Durian ( Durio Zibethinus Murr ) ‘ K Amajaya ’ Lokal Cimahi Secara In Vitro. Kultivasi, 19(1), 1083–1088. Https://Doi.Org/Https://Doi.Org/10.24198/Kultivasi.V19i1.24559 Rahmadi,
Sadili, A., & Sundari, S. (2017). Keanekaragaman , Sebaran , Dan Pemanfaatan Jenis-Jenis Anggrek ( Orchidaceae ) Di Hutan Bodogol , Taman Nasional. Widyariset, 3(2),95–106. Https://Doi.Org/Http://Dx.Doi.Org/10.14203/Widyariset.3.2.2017.95-106 ©
Saputri, W., Mukarlina, & Linda, R. (2015). Respon Pertumbuhan Anggrek Hitam ( Coelogyne Pandurata Lindl .) Secara In-Vitro Dengan Penambahan Ekstrak Tauge Dan Benzyl Amino Purine ( Bap ). Protobiont, 4(2), 84–89.
Serliana, Mukarlina, & Linda, R. (2017). Pertumbuhan Anggrek Hitam ( Coelogyne Pandurata Lindl .) Secara In Vitro Dengan Penambahan Ekstrak Tomat ( Solanum Lycopersicum L ) Dan Benzyl Amino Purine ( Bap ). Protobiont, 6(3), 310–315.
Setiawati, T., Nurzaman, M., Rosmiati, E. S., & Gustianipitaloka, G. (2016). Pertumbuhan Tunas Anggrek Dendrobium Sp. Menggunakan Kombinasi Benzyl Amino Purin (BAP) Dengan Ekstrak Bahan Organik Pada Media Vacin And Went (Vw). Pro-Life, 3(3), 143–152.
Siregar, L. A. M., Sembiring, Shela Lia Nika Br, & Kardhinata, E. Harso. (2017). Perbanyakan Anggrek (Cattleya Trianae Lindl & Rchb.Fil.) Menggunakan Beberapa Komposisi Media Padat Dan Cair Secara In Vitro. Sumatera Utara.
Udomdee, W., Wen, P. J., Lee, C. Y., Chin, S. W., & Chen, F. C. (2014). Effect Of Sucrose Concentration And Seed Maturity On In Vitro Germination Of Dendrobium Nobile Hybrids. Plant Growth Regulation, 72(3), 249–255. Https://Doi.Org/10.1007/S10725-013-9856-X
Yusuf, Y., & Indiarto, A. (2015). Pertumbuhan Dan Perkembangan Embrio Anggrek Vanda Limbata Blume X Vanda Tricolor Lindl . Pada Medium Pupuk Organik Cair Secara In Vitro. Prosiding Seminar Nasional Biologi Dan Pembelajarannya, 533–538.
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Published
2024-12-04
How to Cite
Yuni Astriani Dewi, N. P. ., Yasintha Moi, M. ., & Asti Funan, M. . (2024). TINGKAT KONTAMINASI ANGGREK Vanda limbata DENGAN EKSTRAK BAHAN ALAMI YANG BERBEDA SECARA IN VITRO. Jurnal Biogenerasi, 10(1), 517–523. https://doi.org/10.30605/biogenerasi.v10i1.4942
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