IDENTIFIKASI Candida spp. DARI SAMPEL CAIRAN BRONCHOALVEOLAR LAVAGE (BAL) PASIEN INFEKSI SALURAN PERNAPASAN BAWAH
Indonesia
DOI:
https://doi.org/10.30605/biogenerasi.v8i1.2176Keywords:
Candida, Bronchoalveolar Lavage, Multiplex PCRAbstract
Candida spp. it has been possessed of different virulence and resistance patterns in each species, C. albicans remains the main pathogen in infected hosts, but non-albicans Candida also tries with infection. This study aimed to identify Candida species in the bronchoalveolar lavage fluid (BAL) sample of patients with lower respiratory infections with the method of multiplex PCR (Polymerase Chain Reaction). This study was a descriptive and analytical study with a cross-sectional approach, with BAL samples taken from Wahidin Sudirohusodo Hospital. Identification method using multiplex PCR, beginning with screening through culture as the gold standard. Culture on SDA (Sabouraud Dextrose Agar) media from 103 samples of bronchoalveolar lavage fluid (BAL), including 23 samples (22.3%) positive for Candida spp., and positive results related to genotype with multiplex PCR, including 10/23 (43, 5%) C. albicans, and 5/23 (21.7%) C. albicans + C. glabrata. As much as 65.2 % Candida spp. found in males.
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References
Azoulay E. et al. (2006). Candida colonization of the respiratory tract and subsequent Pseudomonas Ventilator-Associated Pneumonia. CHEST, 129:110–117.
Buoeno E.C., Alicia G.L., Emilia M., Juan L.R.T., & Manuel C.E. (2010). Identification of pathogenic rare yeast species in clinical samples : Comparison between phenotypical and molecular methods. Journal of Clinical Microbiology, Vol. 48.
CDC. (2018). Recommendations for identification of Candida auris. Diakses 12 Januari 2019. Available from : https://www.cdc.gov/fungal/candida-auris/recommendations.html.
Deorukhkar S.C. & Shahriar R. (2018). Identification of Candida species: Conventional methods in the era of molecular diagnosis. Ann Microbiol Immunol, 1(1): 1002.
Ellepola A.N.B. & Christine J.M. (2005). Laboratory diagnosis of invasive Candidiasis. Journal of Microbiology, 43(1),65-84.
Forsberg K. et al. (2018). Candida auris : The recent emergence of a multidrug-resistant fungal pathogen. Medical Mycology, 2018,0,1-12.
Jha B.K., Dey S., Tamang M.D., Joshy M.E., Shivananda P.G., & Brahmadatan, K.N. (2006). Characterization of Candida species isolated from cases of lower respiratory tract infection. Kathmandu University Medical Journal, Vol. 4, No. 3.
Kashefi E., S.J.Seyedi, H. Zarrinfar, A.Fata, H.Mehrad-Majd, & M.J.Najafzadeh. (2021). Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorder. J Babol Univ Med Sci, 23;2021.P:331-336.
Lazaro R. & Hernandez M. (2013). Introduction to the Real-Time PRC. UK : Academic Press.
Madhavan P., Farida J., & Pei P.C. (2011) Laboratory isolation and identification of Candida Species. Journal of Applied Sciences, 11: 2870-2877.
Marinho S.A. et al. (2010). Identification of Candida spp. by phenotypic tests and PCR. Brazilian Journal of Microbiology, 41: 286-294.
Mohammed A., Jumma H.A., & Samir K.A. (2016). Multiplex polymerase chain reaction identification of Candida species colonized sputum of patients suffering from various respiratory tract disorders in Duhok, Iraq. International Journal of Research in Medical Sciences, 4(5):1558-1563.
Mohammed A., Jumma H.A., & Samir K.A. (2017). Identification of Candida spp. isolated from urine by phenotypic methods and multiplex PCR in Duhok, Iraq. Science Journal of University of Zakho, 5(1):11-15.
Pendleton K.M., Gary B.H., & Robert P.D. (2017). The significance of Candida in the human respiratory tract : Our evolving understanding. Pathogens and Disease, Vol. 75, No. 3.
Pinchus D.H., Orenga S., & Chatellier S. (2007). Yeast Identification - Past, present, and future methods. Medical Mycology, 45, 97-121.
Rizwan R., Zahida M.,Shehla S., Faisal A.,Mubarak Z. (2018). Prevalence and Sensitivity Patterns of Candidal Infections in Various Tertiary Care Health Subunits of Karachi. International Journal of Clinical Medicine, 645-659.
Susilawati, Kemas Y.R., Muhaimin R., Theodorus, & Hermansyah. (2019). The Use of Multiplex-PCR method in identification of Candida Species from Vaginal Candidiasis Patients. Biodiversitas, Volume 20, Number 10.
Yang C.W., Barkham T.M.S, Chan F.Y., & Wang Y. (2003). Prevalence of Candida species including Candida dubliniensis in Singapore. Journal of Clinical Microbiology, Vol. 41.
Zarrinfar H., Saeed K., Somayeh D., & Rasoul M. (2016). Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms. Brazilian Journal of Microbiology, 172-176.
Buoeno E.C., Alicia G.L., Emilia M., Juan L.R.T., & Manuel C.E. (2010). Identification of pathogenic rare yeast species in clinical samples : Comparison between phenotypical and molecular methods. Journal of Clinical Microbiology, Vol. 48.
CDC. (2018). Recommendations for identification of Candida auris. Diakses 12 Januari 2019. Available from : https://www.cdc.gov/fungal/candida-auris/recommendations.html.
Deorukhkar S.C. & Shahriar R. (2018). Identification of Candida species: Conventional methods in the era of molecular diagnosis. Ann Microbiol Immunol, 1(1): 1002.
Ellepola A.N.B. & Christine J.M. (2005). Laboratory diagnosis of invasive Candidiasis. Journal of Microbiology, 43(1),65-84.
Forsberg K. et al. (2018). Candida auris : The recent emergence of a multidrug-resistant fungal pathogen. Medical Mycology, 2018,0,1-12.
Jha B.K., Dey S., Tamang M.D., Joshy M.E., Shivananda P.G., & Brahmadatan, K.N. (2006). Characterization of Candida species isolated from cases of lower respiratory tract infection. Kathmandu University Medical Journal, Vol. 4, No. 3.
Kashefi E., S.J.Seyedi, H. Zarrinfar, A.Fata, H.Mehrad-Majd, & M.J.Najafzadeh. (2021). Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorder. J Babol Univ Med Sci, 23;2021.P:331-336.
Lazaro R. & Hernandez M. (2013). Introduction to the Real-Time PRC. UK : Academic Press.
Madhavan P., Farida J., & Pei P.C. (2011) Laboratory isolation and identification of Candida Species. Journal of Applied Sciences, 11: 2870-2877.
Marinho S.A. et al. (2010). Identification of Candida spp. by phenotypic tests and PCR. Brazilian Journal of Microbiology, 41: 286-294.
Mohammed A., Jumma H.A., & Samir K.A. (2016). Multiplex polymerase chain reaction identification of Candida species colonized sputum of patients suffering from various respiratory tract disorders in Duhok, Iraq. International Journal of Research in Medical Sciences, 4(5):1558-1563.
Mohammed A., Jumma H.A., & Samir K.A. (2017). Identification of Candida spp. isolated from urine by phenotypic methods and multiplex PCR in Duhok, Iraq. Science Journal of University of Zakho, 5(1):11-15.
Pendleton K.M., Gary B.H., & Robert P.D. (2017). The significance of Candida in the human respiratory tract : Our evolving understanding. Pathogens and Disease, Vol. 75, No. 3.
Pinchus D.H., Orenga S., & Chatellier S. (2007). Yeast Identification - Past, present, and future methods. Medical Mycology, 45, 97-121.
Rizwan R., Zahida M.,Shehla S., Faisal A.,Mubarak Z. (2018). Prevalence and Sensitivity Patterns of Candidal Infections in Various Tertiary Care Health Subunits of Karachi. International Journal of Clinical Medicine, 645-659.
Susilawati, Kemas Y.R., Muhaimin R., Theodorus, & Hermansyah. (2019). The Use of Multiplex-PCR method in identification of Candida Species from Vaginal Candidiasis Patients. Biodiversitas, Volume 20, Number 10.
Yang C.W., Barkham T.M.S, Chan F.Y., & Wang Y. (2003). Prevalence of Candida species including Candida dubliniensis in Singapore. Journal of Clinical Microbiology, Vol. 41.
Zarrinfar H., Saeed K., Somayeh D., & Rasoul M. (2016). Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms. Brazilian Journal of Microbiology, 172-176.
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Published
2023-01-11
How to Cite
Basri, R. F., Rahbiah Akram, S. ., Rama Yanti, H. ., & Sukma, R. . (2023). IDENTIFIKASI Candida spp. DARI SAMPEL CAIRAN BRONCHOALVEOLAR LAVAGE (BAL) PASIEN INFEKSI SALURAN PERNAPASAN BAWAH: Indonesia. Jurnal Biogenerasi, 8(1), 281–288. https://doi.org/10.30605/biogenerasi.v8i1.2176
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