Jurnal Biogenerasi
type: channel
description: Jurnal Biogenerasi menyajikan artikel orisinal tentang pengetahuan dan informasi riset atau aplikasi riset dan pengembangan dalam bidang biologi dan pendidikan biologi. Bidang kajian Jurnal Biogenerasi meliputi hasil penelitian, uraian metode dan teknik inovasi penelitian serta ulasan/ tinjauan ilmiah mutakhir hasil penelitian dalam bidang di bidang Biologi, edukasi serta pengembangan IPTEK.
publisher: Universitas Cokroaminoto Palopo
language: en
publication name: Jurnal Biogenerasi
issn: 2579-5163
copyright: In submitting the manuscript to the journal, the authors certify that: They are authorized by their co-authors to enter into these arrangements. The work described has not been formally published before, except in the form of an abstract or as part of a published lecture, review, thesis, or overlay journal. That it is not under consideration for publication elsewhere, That its publication has been approved by all the author(s) and by the responsible authorities – tacitly or explicitly – of the institutes where the work has been carried out. They secure the right to reproduce any material that has already been published or copyrighted elsewhere. They agree to the following license and copyright agreement. License and Copyright Agreement Authors who publish with this journal agree to the following terms: Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
items: Ferns (Pteridophyta) Diversity in West Kalimantan: A Literature-Based Study
items: Analisis Perbandingan Ekstrak Kulit Buah Naga (Hylocereus polyrhizus) dan Kunyit (Curcuma longa L.) Sebagai Indikator Identifikasi Boraks Pada Makanan
items: Keanekaragaman Jenis Mangrove Di Kawasan Hutan Mangrove Kota Tarakan
items: Keanekaragaman Isolat Bakteri Rizosfer Tanaman Jagung (Zea mays L.) pada Tanah Masam di Kabupaten Maros
items: KARAKTERISASI ISOLAT BAKTERI RIZOSFER JAGUNG HIBRIDA (Zea mays L.) PADA TANAH MASAM
items: ANALISIS FECAL COLIFORM PADA AIR LIMBAH MENGGUNAKAN METODE MOST PROBABLE NUMBER (MPN)
items: Analisis Coliform Pada Sampel Air Limbah Menggunakan Metode Most Probable Number
items: Analisis Cemaran Salmonella pada Makanan dengan Metode Compact Dry melalui Media Buffered Peptone Water
items: PENGARUH AIR SUMUR DAN AIR SABUN TERHADAP AKTIVITAS MITOSIS SEL AKAR BAWANG (Allium cepa L.
items: IMPLEMENTASI PEMBELAJARAN IPA BERBASIS ONLINE LEARNING BERBANTUAN E-BOOK INTERAKTIF UNTUK MENINGKATKAN KETERAMPILAN BERPIKIR KREATIF
items: Analisis Cemaran Escherichia coli pada Sampel Makanan Menggunakan Metode Compact Dry
items: Evaluasi Ekstrak Bunga Asoka sebagai Indikator Alami Deteksi Boraks pada Sampel Makanan Olahan
items: STABLE ISOTOPE FINGERPRINTING OF RICE VARIETIES AND CULTIVATION SYSTEMS USING EA–IRMS
items: PENGARUH MEDIA TANAM SERBUK GERGAJI DAN COCOPEAT TERHADAP PERTUMBUHAN DAUN PADA PENCANGKOKAN TANAMAN MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.)
items: ANALISIS PEMBELAJARAN PENDIDIKAN LINGKUNGAN DIINTEGRASIKAN MATA PELAJARAN IPA BERBASIS TEKNOLOGI INFORMASI DI SMK NEGERI 2 PALOPO
items: Profil Keterampilan Praktikum Hukum Newton pada Mata Kuliah IPA Sekolah 1 menggunakan Tes Tulis
items: ANALISIS PERBANDINGAN KEBUTUHAN OKSIGEN (O2) DALAM PROSES RESPIRASI ANTARA TUMBUHAN ANGIOSPERMAE DAN GYMNOSPERMAE
items: Assessment of Biology Laboratory Management and Facilities in Public Senior High Schools
items: TINJAUAN FITOKIMIA DAN AKTIVITAS ANTIDIABETES Cinnamomum zeylanicum Blume PADA DIABETES MELITUS TIPE 2 : LITERATUR REVIEW
items: LITERATUR REVIEW: APLIKASI MOLEKULER BERBASIS PCR DALAM DETEKSI DAN KARAKTERISASI Escherichia coli PADA SEKTOR LINGKUNGAN, PANGAN, DAN KLINIS
items: DISTRIBUSI DAN KARAKTERISTIK SAMPAH WISATA DI ZONA INTERTIDAL LABUAN BAJO SERTA IMPLIKASI AWAL TERHADAP EKOSISTEM PESISIR
items: INVENTARISASI TANAMAN HIAS DI KAMPUS UNIVERSITAS NEGERI PADANG
items: Status Permudaan Alami Hutan Mangrove di Teluk Buo Bungus Kota Padang
items: Literature Review: Bakteri Asam Laktat Dari Dadih Minangkabau: Keragaman, Karakteristik, Dan Potensi Aktivitas Antibakteri
items: Integrasi Alat Bioinformatika Dalam Kurikulum Genetika Molekuler: Meningkatkan Kompetensi Abad 21 Mahasiswa
items: INDUKSI TUNAS Musa paradisiaca L. MENGGUNAKAN KOMBINASI IBA DAN BAP SERTA AIR KELAPA
items: LITERATURE REVIEW: KARAKTERISTIK DAN DISTRIBUSI CORONAVIRUS PADA KELELAWAR SEBAGAI RESERVOIR ALAMI
items: JENIS-JENIS TANAMAN HORTIKULTURA DI DESA SINDANG KASIH KECAMATAN RANOMEETO BARAT KABUPAEN KONAWE SELATAN
items: Analisis Keterampilan Metakognitif Siswa Kelas VIII SMP Makassar Raya dalam Menjawab Soal Berbasis HOTS
items: Efek Seed Priming KNO3 terhadap Perkecambahan dan Pertumbuhan Awal Benih Padi Varietas IR 64 pada Beberapa Tingkat Salinitas

Ferns (Pteridophyta) Diversity in West Kalimantan: A Literature-Based Study
type: item
description: Indonesia is a tropical country that has a diversity of flora and fauna that must be preserved and developed, one of which is ferns. Ferns can grow well in open ground. With sufficient sunlight, acidity (pH) of 3.5–6.5, and temperature of 21-27°C, ferns can grow after forest fires or forests covered by tree canopies. West Kalimantan has a humid tropical climate, diverse habitats, nutrient-rich soil, dense forest canopy, lack of human disturbance, and complex natural ecological processes. Ferns grow and live in a variety of species in the forests of West Kalimantan. The approach used is a literature study, namely looking for primary sources or literature in the form of national and international journals in the previous decade (2014-2024). There are 16 families with 34 species. The most commonly found species come from the Polypodiaceae family. This article can be used as a source of information to support additional research on ferns.
subject: Articles
  topic: section
subject: Pteridophyta,
  topic: keywords
subject: Kalimantan Barat
  topic: keywords
creator: Rifki Aldiansyah
creator: Syamswisna
creator: Audiva Azhta Prameswari
creator: Irvan Ariyansyah
creator: Putri Anggini
creator: Angel Acum Diarsih
rights: Copyright (c) 2024 Rifki Aldiansyah, Syamswisna, Audiva Azhta Prameswari, Irvan Ariyansyah, Putri Anggini, Angel Acum Diarsih https://creativecommons.org/licenses/by/4.0
date: 2024-06-16
publication date: 2024-06-16
starting page: 1170
ending page: 1176
doi: 10.30605/t3w8r731

Analisis Perbandingan Ekstrak Kulit Buah Naga (Hylocereus polyrhizus) dan Kunyit (Curcuma longa L.) Sebagai Indikator Identifikasi Boraks Pada Makanan
type: item
description: This study aimed to analyze and compare the effectiveness of red dragon fruit peel (Hylocereus polyrhizus) extract and turmeric (Curcuma longa L.) extract as natural indicators for detecting borax in food samples. The research used a laboratory experimental method with a qualitative descriptive approach. Food samples tested included sausages, ketupat, lontong, white tofu, crackers, siomay, meatballs, cilok, pempek, nuggets, white noodles, and yellow noodles. The testing process was conducted by dripping dragon fruit peel extract and turmeric extract onto each sample and observing the resulting color changes. The results showed that most samples tested negative for borax, while meatballs and yellow noodles indicated positive results. The color change in turmeric to reddish-brown occurred due to the reaction between curcumin and borax, while dragon fruit peel extract changed color because anthocyanin pigments are sensitive to pH changes. The findings indicate that both natural materials can be used as simple, inexpensive, safe, and effective natural indicators for the qualitative preliminary detection of borax in food. However, further quantitative testing is needed to obtain more accurate results.
subject: Articles
  topic: section
subject: boraks
  topic: keywords
subject: kulit buah naga
  topic: keywords
subject: kunyit
  topic: keywords
subject: antosianin
  topic: keywords
subject: indikator alami
  topic: keywords
creator: Claudia Nainggolan
creator: Eka Setiawan
creator: Emon Sejahtera Zendrato
creator: Grecia Trinatal Simbolon
creator: Kania Restya Diva
creator: Larasati Arum Utami
creator: Rejeki Sihite
rights: Copyright (c) 2026 Claudia Purba, Eka Setiawan, Emon Sejahtera Zendrato, Grecia Trinatal Simbolon, Kania Restya Diva, Larasati Arum Utami, Rejeki Sihite https://creativecommons.org/licenses/by/4.0
date: 2026-06-15
publication date: 2026-06-15
starting page: 872
ending page: 878
doi: 10.30605/sbjm5j61

Keanekaragaman Jenis Mangrove Di Kawasan Hutan Mangrove Kota Tarakan
type: item
description: Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis mangrove yang tumbuh di Kawasan Konservasi Hutan Mangrove Kota Tarakan. Pengambilan data dalam penelitian ini menggunakan metode Purposive Sampling dengan cara menarik garis transek mengarah tegak lurus dari garis Pantai ke arah darat dan pertimbangan kerapatan vegetasi dengan pengampilan sampel dilakukan pada 3 stasiun. Hasil penelitian tentang keanekaragaman jenis mangrove yang tumbuh di Kawasan Konservasi Kota Tarakan menunjukkan bahwa jenis mangrove yang ditemukan sebanyak 7 jenis dan yang paling dominan adalah jenis mangrove Rhizophora Appiculata. Berdasarkan hasil analisis data dapat disimpulkan bahwa indeks keanekaragaman Jenis Mangrove di Kawasan Konservasi Hutan Mangrove dan Bekantan Di Kota Tarakan sebesar 1<H’≤1.8276 yang berarti tergolong kedalam kriteria sedang.
subject: Articles
  topic: section
subject: Mangrove
  topic: keywords
subject: Keanekaragaman
  topic: keywords
subject: Kawasan Konservasi
  topic: keywords
creator: Suang Andi Palimbong
creator: Raflen Aril Gerungan
creator: Subekti Nurmawati
creator: Elizabeth Novi Kusumaningrum
creator: Mustaking
rights: Copyright (c) 2026 Suang Andi Palimbong, Raflen Aril Gerungan, Subekti Nurmawati, Elizabeth Novi Kusumaningrum, Mustaking https://creativecommons.org/licenses/by/4.0
date: 2026-06-13
publication date: 2026-06-13
starting page: 824
ending page: 830
doi: 10.30605/b1qaq858

Keanekaragaman Isolat Bakteri Rizosfer Tanaman Jagung (Zea mays L.) pada Tanah Masam di Kabupaten Maros
type: item
description: This study aimed to the diversity of rhizosphere bacterial isolates associated with maize (Zea mays L.) cultivated in acidic soils of Maros Regency, South Sulawesi Province. Acidic soils characterized by low pH and limited nutrients, but the ability of microorganisms can adapt according to their habitat. However, microorganisms, particularly rhizosphere bacteria, exhibit remarkable adaptability to such conditions. The research employed serial dilution and culture on Nutrient Agar (NA) media to isolate bacteria from rhizosphere soil samples. A total of 14 bacterial isolates were toward will different morphological characteristics. All bacterial isolates found are Gram-positive bacteria. The findings reveal a high diversity of rhizosphere bacterial isolates, suggesting their potential role in enhancing plant growth as biofertilizers. Further research is necessary to evaluate the physiological and molecular potential of these bacterial isolates in nitrogen fixation, phosphate solubilization, and the production of plant growth hormones. Additionally, exploring the potential application of these bacteria as biocontrol agents or biofertilizers could contribute to improving maize productivity in acidic soils
subject: Articles
  topic: section
subject: Bacteria
  topic: keywords
subject: Rhizosphere
  topic: keywords
subject: Maize
  topic: keywords
subject: Acidic
  topic: keywords
creator: Akhmad Syakur
rights: Copyright (c) 2025 Akhmad Syakur https://creativecommons.org/licenses/by/4.0
date: 2025-12-29
publication date: 2025-12-29
starting page: 2358
ending page: 2365
doi: 10.30605/w0nnv838

KARAKTERISASI ISOLAT BAKTERI RIZOSFER JAGUNG HIBRIDA (Zea mays L.) PADA TANAH MASAM
type: item
description: Acidic soil is one of the major constraints in maize cultivation because low soil pH can increase the solubility of Al and Fe, inhibit root growth, and reduce nutrient availability, particularly phosphorus. Rhizosphere bacteria have the potential to support plant growth through various mechanisms; therefore, the exploration of local bacterial isolates from acidic soil environments is important. This study aimed to isolate and characterize rhizosphere bacteria associated with hybrid maize grown in acidic soil in Moncongloe Village, Maros Regency. Bacterial isolation was carried out using serial dilution and cultivation on Nutrient Agar (NA) medium, followed by colony purification and characterization based on colony morphology, Gram reaction using 3% KOH, and catalase activity using 3% H₂O₂. The results showed that 14 bacterial isolates were obtained from maize rhizosphere samples for further characterization. All isolates showed a Gram-positive reaction, while the catalase test revealed that 11 isolates (78.57%) were catalase-positive and 3 isolates (21.43%) were catalase-negative. Differences in morphological and basic biochemical characteristics indicate the diversity of rhizosphere bacterial isolates capable of growing under acidic soil conditions. These isolates have the potential to serve as local microbial candidates for biofertilizer development; however, further studies are required to evaluate their PGPR functional traits, biosafety, and molecular identification.
subject: Articles
  topic: section
subject: hybrid maize
  topic: keywords
subject: acidic soil
  topic: keywords
subject: rhizosphere
  topic: keywords
subject: bacterial isolation
  topic: keywords
subject: characterization
  topic: keywords
creator: Akhmad Syakur
rights: Copyright (c) 2025 Akhmad Syakur https://creativecommons.org/licenses/by/4.0
date: 2025-12-24
publication date: 2025-12-24
starting page: 2350
ending page: 2357
doi: 10.30605/cq4z0s16

ANALISIS FECAL COLIFORM PADA AIR LIMBAH MENGGUNAKAN METODE MOST PROBABLE NUMBER (MPN)
type: item
description: Domestic wastewater has the potential to pollute the environment because it contains pathogenic microorganisms, including fecal coliform bacteria as indicators of biological contamination. This study aimed to determine the presence of fecal coliform in wastewater samples using the Most Probable Number (MPN) method. The research was conducted from February 2 to February 6, 2026, at the Public Health Laboratory Center (BLKM) Medan. The study used a descriptive method with a five-tube MPN technique through presumptive testing using Lauryl Tryptose Broth (LTB) medium and confirmed testing using EC Broth referring to EPA Method 1680. The results showed variations in fecal coliform MPN values in each wastewater sample. Samples AL8, AL9, AL11, and AL12 showed the highest MPN value of >1600 MPN/100 mL, while samples AL1, AL2, and AL6 showed the lowest MPN value of <1.8 MPN/100 mL. The MPN method using LTB and EC Broth media was effective in detecting fecal coliform contamination in wastewater samples.
subject: Articles
  topic: section
subject: Air limbah
  topic: keywords
subject: fecal coliform
  topic: keywords
subject: Most Probable Number
  topic: keywords
subject: Lauryl tryptose broth
  topic: keywords
subject: EC Broth
  topic: keywords
creator: Siti Nabila Nabila
creator: Endang Sulistyarini Gultom
rights: Copyright (c) 2026 Siti Nabila Nabila, Endang Sulistyarini Gultom https://creativecommons.org/licenses/by/4.0
date: 2026-06-14
publication date: 2026-06-14
doi: 10.30605/xmcbmm96

Analisis Coliform Pada Sampel Air Limbah Menggunakan Metode Most Probable Number
type: item
description: Penelitian ini bertujuan untuk menganalisis keberadaan bakteri coliform pada sampel air limbah menggunakan metode Most Probable Number (MPN). Air limbah yang tidak diolah dengan baik berpotensi menjadi sumber pencemaran lingkungan dan penyebaran mikroorganisme patogen. Digunakann media Lauryl Tryptose Broth (LTB) pada uji pendugaan dan Brilliant Green Lactose Bile Broth (BGLB) pada uji penegasan. Sebanyak 15 sampel air limbah dianalisis untuk menentukan jumlah bakteri coliform. Hasil penelitian menunjukkan bahwa sebagian besar sampel terkontaminasi bakteri coliform dengan nilai MPN yang bervariasi antara <1,8 hingga >1600 MPN/100 mL. Beberapa sampel menunjukkan tingkat pencemaran yang sangat tinggi, sedangkan beberapa lainnya tidak terdeteksi mengandung coliform. Perbedaan tingkat pencemaran dipengaruhi oleh kondisi sanitasi, kandungan bahan organik, suhu, dan karakteristik sumber limbah. Hasil penelitian menunjukkan bahwa sebagian besar sampel belum memenuhi standar kualitas mikrobiologis sehingga diperlukan pengelolaan limbah yang lebih baik.
subject: Articles
  topic: section
subject: Coliform
  topic: keywords
subject: Air limbah
  topic: keywords
subject: Most Probable Number
  topic: keywords
subject: Pencemaran mikrobiologis
  topic: keywords
subject: Kualitas air
  topic: keywords
creator: Deka Wira Pratama
creator: Endang Sulistyarini Gultom
rights: Copyright (c) 2026 Deka Wira Pratama, Endang Sulistyarini Gultom https://creativecommons.org/licenses/by/4.0
date: 2026-06-13
publication date: 2026-06-13
starting page: 854
ending page: 859
doi: 10.30605/emwhgg03

Analisis Cemaran Salmonella pada Makanan dengan Metode Compact Dry melalui Media Buffered Peptone Water
type: item
description: Keamanan pangan merupakan aspek krusial dalam menjaga kesehatan masyarakat dari penyakit bawaan makanan (foodborne disease) akibat kontaminasi bakteri patogen seperti Salmonella sp. pada berbagai tahapan pangan, khususnya produk hewani yang tidak diproses secara higienis. Infeksi bakteri Gram negatif ini dapat menyebabkan salmonellosis dengan gejala gangguan pencernaan hingga komplikasi serius pada kelompok rentan, sehingga pemeriksaan mikrobiologi pangan sangat penting untuk melindungi konsumen. Dalam analisisnya, tahap pra-pengkayaan (pre-enrichment) menggunakan media cair non-selektif Buffer Peptone Water (BPW) menjadi langkah awal yang sangat vital untuk memulihkan sel bakteri yang stres atau rusak akibat pengolahan, sekaligus meningkatkan sensitivitas deteksinya. Oleh karena itu, kajian ini bertujuan untuk membahas prosedur pemeriksaan Salmonella sp. pada sampel makanan serta teknik pembuatan media BPW. Melalui pemahaman metode ini, diharapkan pengetahuan mengenai pengujian mikrobiologi pangan dapat meningkat sebagai upaya preventif terhadap kontaminasi bakteri pada makanan.
subject: Articles
  topic: section
subject: Keamanan pangan
  topic: keywords
subject: Salmonella sp
  topic: keywords
subject: Buffer Peptone Water
  topic: keywords
subject: Pra-pengkayaan
  topic: keywords
subject: Mikrobiologi pangan
  topic: keywords
creator: Valdo Rekson Nainggolan
creator: Endang Sulistyarini Gultom
rights: Copyright (c) 2026 Valdo Rekson Nainggolan, Endang Sulistyarini Gultom https://creativecommons.org/licenses/by/4.0
date: 2026-06-13
publication date: 2026-06-13
starting page: 847
ending page: 853
doi: 10.30605/t6d4j218

PENGARUH AIR SUMUR DAN AIR SABUN TERHADAP AKTIVITAS MITOSIS SEL AKAR BAWANG (Allium cepa L.
type: item
description: Mitosis is a cell division process that plays an important role in plant growth. This study aimed to determine the effect of well water and soapy water on the mitotic activity of onion root cells (Allium cepa L.). This research used a descriptive observational method through laboratory practicum with onion roots as the research object. Data were collected through direct observation using a light microscope on mitotic phases of onion root preparations stained with acetocarmine. The results showed that well water produced better root growth and mitotic activity than soapy water. The phases of prophase, metaphase, anaphase, and telophase were more clearly observed in the well water treatment, while fewer dividing cells and damaged tissues were found in the soapy water treatment. It can be concluded that soapy water has a negative effect on the mitotic activity of onion root cells.
subject: Articles
  topic: section
subject: Mitosis
  topic: keywords
subject: onion root
  topic: keywords
subject: well water
  topic: keywords
subject: soapy water
  topic: keywords
subject: Allium cepa L
  topic: keywords
creator: Christian Yopie Daniel Siregar Siagian
creator: Siti Annisa Kusbama
creator: Khory Aulia
creator: Nazla Suhaila Parinduri
creator: Santi Dameana Habeahan
creator: Larasati Arum Utami
creator: Hestia Hairima
rights: Copyright (c) 2026 Christian Yopie Daniel Siregar Siagian, Siti Annisa Kusbama, Khory Aulia, Nazla Suhaila Parinduri, Santi Dameana Habeahan, Larasati Arum Utami, Hestia Hairima https://creativecommons.org/licenses/by/4.0
date: 2026-06-08
publication date: 2026-06-08
starting page: 809
ending page: 815
doi: 10.30605/98kk3z97

IMPLEMENTASI PEMBELAJARAN IPA BERBASIS ONLINE LEARNING BERBANTUAN E-BOOK INTERAKTIF UNTUK MENINGKATKAN KETERAMPILAN BERPIKIR KREATIF
type: item
description: Digital transformation in education encourages the integration of technology in science learning to support the development of 21st-century skills, one of which is creative thinking skills. This study aimed to determine the improvement of students’ creative thinking skills through the implementation of science learning assisted by an interactive e-book on the subtopic of Nutrition in Food. This study employed a pre-experimental research design using the One Group Pretest-Posttest Design. The research sample consisted of 23 eighth-grade students of SMP Piri 1 Yogyakarta selected using a saturated sampling technique. Data were collected using an essay-based creative thinking skills test covering the aspects of fluency, flexibility, originality, and elaboration. The data were analyzed using descriptive quantitative analysis with n-gain calculation. The results showed that the implementation of science learning assisted by an interactive e-book was able to improve students’ creative thinking skills. The n-gain results indicated that 3 students were categorized as high and 20 students were categorized as moderate. Improvements also occurred in each aspect of creative thinking skills, namely fluency by 30.5%, flexibility by 18.9%, originality by 26.1%, and elaboration by 22.3%. The use of interactive e-books in science learning was able to create a more engaging, interactive, and flexible learning experience, thereby supporting the development of students’ creative thinking skills.
subject: Articles
  topic: section
subject: science learning; online learning; interactive e-book; creative thinking skills; digital learning.
  topic: keywords
creator: Nur Rahmah Sangkala
creator: Musawwir Usman
creator: Akhmad Syakur
rights: Copyright (c) 2026 Nur Rahmah Sangkala, Musawwir Usman, Akhmad Syakur https://creativecommons.org/licenses/by/4.0
date: 2026-05-05
publication date: 2026-05-05
starting page: 788
ending page: 797
doi: 10.30605/bykr9k12

Analisis Cemaran Escherichia coli pada Sampel Makanan Menggunakan Metode Compact Dry
type: item
description: Food safety is a critical aspect of protecting public health from foodborne diseases caused by microbiological contamination, with Escherichia coli serving as a key indicator of food hygiene and sanitation. This study aimed to analyze Escherichia coli contamination in food samples using the Compact Dry method and to evaluate its compliance with food safety standards. A descriptive method with a quantitative approach was employed on 20 food samples, which were tested using Compact Dry EC and incubated at 36 ± 0.5°C for 21 ± 2 hours. The results were expressed in CFU/g and compared with the standards set by SNI 7388:2009. The findings showed that 45% of the samples were positive for Escherichia coli, with contamination levels ranging from <1 to 1.25 × 10³ CFU/g, while 55% were negative. All positive samples did not meet food safety standards. Contamination was likely due to poor hygiene and sanitation practices, including cross-contamination during food processing and handling. The Compact Dry method proved to be effective, practical, and efficient for detecting Escherichia coli. These findings indicate that microbiological contamination in food remains a concern, highlighting the need to improve hygiene and sanitation practices to ensure food safety.
subject: Articles
  topic: section
subject: Escherichia coli
  topic: keywords
subject: Food Safety
  topic: keywords
subject: Compact Dry
  topic: keywords
subject: Microbial contamination
  topic: keywords
subject: CFU/g
  topic: keywords
creator: Warda Amalia
creator: Endang Sulistyarini Gultom Endang Sulistyarini Gultom
rights: Copyright (c) 2026 Warda Amalia, Dr. Endang Sulistyarini Gultom S.Si., M.Si., Apt https://creativecommons.org/licenses/by/4.0
date: 2026-06-13
publication date: 2026-06-13
starting page: 841
ending page: 846
doi: 10.30605/3kbf2x84

Evaluasi Ekstrak Bunga Asoka sebagai Indikator Alami Deteksi Boraks pada Sampel Makanan Olahan
type: item
description: Borax is a prohibited food additive but is still found in processed foods in Indonesia. This study aims to evaluate Saraca asoca leaf extract as a natural indicator for borax detection. The extract was prepared using 70% ethanol and tested on borax solutions and food samples. The results showed a color change from brownish-green to yellow with increasing borax concentration, starting at 1.0%. Similar changes in several samples indicated the presence of borax. This change is related to interactions between flavonoid and tannin compounds and borate ions. Therefore, Saraca asoca extract has potential as a simple natural indicator for visual borax detection
subject: Articles
  topic: section
subject: borax, natural indicator, processed food, Saraca asoca, visual detection
  topic: keywords
creator: Desy Purwaty
creator: Sartika Magdalena Sihite
creator: Ilham Pratama
creator: Santa Ceacilia Br Tarigan
creator: Stevani Putri Christie Gulo
creator: Eka Setiawan
creator: Larasati Arum Utami
rights: Copyright (c) 2026 Desy Purwaty, Sartika Magdalena Sihite, Ilham Pratama, Santa Ceacilia Br Tarigan, Stevani Putri Christie Gulo, Eka Setiawan, Larasati Arum Utami https://creativecommons.org/licenses/by/4.0
date: 2026-06-14
publication date: 2026-06-14
starting page: 866
ending page: 871
doi: 10.30605/fxv1ad60

STABLE ISOTOPE FINGERPRINTING OF RICE VARIETIES AND CULTIVATION SYSTEMS USING EA–IRMS
type: item
description: Rice (Oryza sativa L.) is a staple food for more than half of the global population and is available in various types, including white, red, black, brown, and basmati rice. Authentication of rice varieties and cultivation systems is increasingly important for ensuring food quality and preventing fraud. This study aimed to characterize the stable isotope composition of different rice types and to evaluate isotopic differences between organic and conventional cultivation systems using Elemental Analyzer–Isotope Ratio Mass Spectrometry (EA–IRMS). Carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope ratios were measured in seven rice samples. The results showed that δ¹³C values ranged from −30.33‰ to −28.06‰, confirming that all samples belong to the C₃ photosynthetic group, while δ¹⁵N values ranged from 4.29‰ to 6.05‰, reflecting variability in nitrogen sources and soil processes. Each rice type exhibited a distinct isotopic profile, indicating the potential of isotope ratios as fingerprints for varietal differentiation. However, isotopic differences between organic and conventional samples were not consistently distinguishable due to overlapping values. These findings suggest that stable isotope analysis has potential as a tool for rice authentication in the Indonesian market. Nevertheless, the limited sample size in this study indicates that the results should be considered preliminary, and further validation using larger datasets and multivariate statistical approaches is required to improve classification accuracy.
subject: Articles
  topic: section
subject: isotope ratio
  topic: keywords
subject: EA–IRMS
  topic: keywords
subject: rice authentication
  topic: keywords
subject: δ13C
  topic: keywords
subject: δ15N
  topic: keywords
creator: Rahmad Ramdani Sambari
creator: Na’ilah Insani Alifiyah
rights: Copyright (c) 2026 Rahmad Ramdani Sambari, Na’ilah Insani Alifiyah https://creativecommons.org/licenses/by/4.0
date: 2026-06-09
publication date: 2026-06-09
starting page: 816
ending page: 823
doi: 10.30605/58rh2z84

PENGARUH MEDIA TANAM SERBUK GERGAJI DAN COCOPEAT TERHADAP PERTUMBUHAN DAUN PADA PENCANGKOKAN TANAMAN MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.)
type: item
description: The mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) plant is a medicinal plant with high economic and pharmacological value, so effective propagation techniques are needed to increase its production. One method used is grafting, the success of which is influenced by the planting medium. Based on this, a study was conducted on the effect of sawdust and cocopeat growing media on leaf growth in grafting crown of the gods plant. The study was conducted in January 2026 in the Lubuk Minturun area of ​​Padang City. This study used a Completely Randomized Design (CRD) with three treatments: soil (P0), sawdust (P1), and cocopeat (P2), each with six replications, resulting in 18 experimental units. Observed parameters included leaf number and leaf dry weight. Data were analyzed using analysis of variance (ANOVA). The results showed that the planting medium used had no significant effect on leaf number or dry weight.
subject: Articles
  topic: section
subject: mahkota dewa
  topic: keywords
subject: air layering
  topic: keywords
subject: sawdust
  topic: keywords
subject: cocopeat
  topic: keywords
subject: leaf growth
  topic: keywords
creator: Suci Syafputri
creator: Vauzia
creator: Irma Leilani Eka Putri
creator: Filza Yulina Ade
rights: Copyright (c) 2026 Suci Syafputri, Vauzia, Irma Leilani, Eka Putri, Filza Yulina Ade https://creativecommons.org/licenses/by/4.0
date: 2026-06-19
publication date: 2026-06-19
starting page: 898
ending page: 905
doi: 10.30605/g6a7z493

ANALISIS PEMBELAJARAN PENDIDIKAN LINGKUNGAN DIINTEGRASIKAN MATA PELAJARAN IPA BERBASIS TEKNOLOGI INFORMASI DI SMK NEGERI 2 PALOPO
type: item
description: The purpose of this study was to determine the learning outcomes of environmental education integrated with science subjects using information technology applications on domestic waste, wastewater sanitation, and air pollution, which teachers currently find difficult to achieve. The study used an action method, namely: (1) the design phase of lesson plans, information technology devices, and learning materials; (2) the implementation phase of learning devices validated by education experts and information technology experts for suitability before being implemented in 35 classes using a purposive sampling technique; (3) the evaluation phase, which presented the validation results of the lesson plans for all components assessed in the very valid category with an average score of 3.7. The information technology application validation results were in the very valid category with an average score of 3.7. The learning outcome data were analyzed in descriptive statistics, obtained an average learning outcome of 76.0 for domestic waste, soil pollution, and air pollution. 31 students achieved a high level of mastery of environmental education learning materials, representing 88.4% of the students, indicating that they achieved a high level of mastery of the learning outcomes for domestic waste, soil pollution, and pollution. It is concluded that integrating environmental education into science subjects in vocational schools using information technology media can overcome teachers' difficulties in presenting material and evaluating mastery.
subject: Articles
  topic: section
subject: Environmental education
  topic: keywords
subject: learning outcomes
  topic: keywords
subject: action methods
  topic: keywords
creator: Asri
rights: Copyright (c) 2026 Asri https://creativecommons.org/licenses/by/4.0
date: 2026-06-19
publication date: 2026-06-19
starting page: 889
ending page: 897
doi: 10.30605/0q0ndw91

Profil Keterampilan Praktikum Hukum Newton pada Mata Kuliah IPA Sekolah 1 menggunakan Tes Tulis
type: item
description: This study aims to describe the practical skills profile of prospective science teacher students on Newton’s Laws through a written-test-based final examination. This research employed a quantitative descriptive approach with a total sampling technique involving 92 valid students from three classes. The instrument consisted of multiple-choice, sequencing, classification, and image-based identification items designed to measure four aspects: identifying tools and materials, assembling apparatus, reading measuring instruments, and analyzing observation results. The data were analyzed descriptively using categorization based on score ranges. The results showed that students’ overall practical skills were in the good category, with an average score of 68.6%. The findings for each aspect were as follows: (1) reading measuring instruments achieved the highest score (75.7%); (2) followed by identifying tools and materials (72.7%); (3) assembling apparatus (58.7%); and (4) analyzing observation results (42.7%), with the latter two aspects falling into the Fair category. These findings imply the need to strengthen instruction in data analysis skills and procedural understanding of assembling experimental apparatus.
subject: Articles
  topic: section
subject: Practical skills
  topic: keywords
subject: Newton’s Laws
  topic: keywords
subject: science education
  topic: keywords
subject: written test
  topic: keywords
subject: quantitative descriptive
  topic: keywords
creator: Shoffan Fatkhulloh
creator: Nurhayani H. Muhiddin
creator: Putri damayanti
rights: Copyright (c) 2026 Shoffan Fatkhulloh, Nurhayani H. Muhiddin, Putri damayanti https://creativecommons.org/licenses/by/4.0
date: 2025-05-20
publication date: 2025-05-20
starting page: 779
ending page: 787
doi: 10.30605/9p53ra19

ANALISIS PERBANDINGAN KEBUTUHAN OKSIGEN (O2) DALAM PROSES RESPIRASI ANTARA TUMBUHAN ANGIOSPERMAE DAN GYMNOSPERMAE
type: item
description: Penelitian ini bertujuan untuk membandingkan kebutuhan oksigen (O₂) dalam proses respirasi antara tumbuhan dari kelompok Angiospermae dan Gymnospermae. Penelitian ini dilakukan melalui metode eksperimen menggunakan alat respirometer yang dilaksanakan di Laboratorium Biologi Universitas Maritim Raja Ali Haji pada bulan Mei tahun 2026. Sampel yang diteliti meliputi Hibiscus rosa-sinensis dan Ipomoea Aquatica yang mewakili Angiospermae, serta Pinus merkusii dan Cycas revoluta untuk Gymnospermae. Pengamatan dilakukan dua kali pada setiap sampel dengan menggunakan KOH untuk menyerap karbon dioksida (CO₂) dan larutan eosin sebagai indikator perubahan volume gas di respirometer. Data yang diperoleh dianalisis menggunakan uji ANOVA untuk melihat perbedaan konsumsi oksigen di antara kedua kelompok tumbuhan tersebut. Hasil menunjukkan bahwa rata-rata konsumsi oksigen pada Gymnospermae adalah 0,95 ml, sedangkan Angiospermae mencatat 0,88 ml. Meski Gymnospermae menunjukkan laju respirasi yang cenderung lebih tinggi, analisis ANOVA menemukan bahwa perbedaan ini tidak signifikan secara statistik karena nilai F-hitung (0,3741) lebih kecil dari F-tabel pada tingkat signifikansi 5% dan 1%. Studi ini mengindikasikan bahwa perbedaan laju respirasi dipengaruhi oleh faktor fisiologis, struktur anatomi, metabolisme, serta kondisi lingkungan saat pengamatan. Oleh karena itu , dapat disimpulkan bahwa kebutuhan oksigen dalam proses respirasi antara kelompok Angiospermae dan Gymnospermae tidak menunjukkan perbedaan yang signifikan.
subject: Articles
  topic: section
subject: respirasi tumbuhan, oksigen, Angiospermae, Gymnospermae, respirameter
  topic: keywords
creator: Hairummuslimah Qurays
creator: Erdi Ananda
creator: Helmi Putri
creator: Risma Yunika
creator: Riski Amelia
creator: Rohana Desma Yunita
creator: Erda Muhartati
rights: Copyright (c) 2026 Hairummuslimah Qurays, Erdi Ananda, Helmi Putri, Risma Yunika, Riski Amelia, Rohana Desma Yunita, Erda Muhartati https://creativecommons.org/licenses/by/4.0
date: 2026-06-13
publication date: 2026-06-13
starting page: 831
ending page: 840
doi: 10.30605/b7bbrk78

Assessment of Biology Laboratory Management and Facilities in Public Senior High Schools
type: item
description: Biology laboratories play an important role in supporting practical learning activities and developing students’ scientific skills in science education. Effective laboratory management and adequate facilities are necessary to ensure that practical activities are conducted safely and optimally. This study aimed to analyse: (1) the management of biology laboratories, (2) the completeness of biology laboratory facilities and materials, and (3) the implementation of laboratory management practices in public senior high schools in Malang City. This study employed a descriptive research design using percentage-based statistical analysis. The population consisted of 10 public senior high schools in Malang City, and 3 schools were purposively selected based on school zoning representation and Accreditation A status. The respondents included biology teachers, laboratory heads, and laboratory assistants. Data were collected through observations, questionnaires, interviews, and documentation. The research instruments were validated through expert judgment by biology education lecturers. The results showed that overall management of biology laboratories was rated very good, with an average score of 87.69%. Material ordering, storage, and safety and maintenance management were generally implemented effectively. The completeness of biology laboratory facilities and materials was also categorised as very complete, with an average percentage of 89.20%. However, several weaknesses remained, particularly in hazardous waste disposal, laboratory safety practices, and infrastructure maintenance. Overall, biology laboratories in public senior high schools in Malang City were adequately managed and equipped to support biology practical learning activities.
subject: Articles
  topic: section
subject: Biology Laboratory
  topic: keywords
subject: Laboratory Management
  topic: keywords
subject: Laboratory Facilities
  topic: keywords
subject: Practical Learning
  topic: keywords
subject: Senior High School
  topic: keywords
creator: Lianto
creator: Novela Memiasih
rights: Copyright (c) 2026 Lianto, Novela Memiasih https://creativecommons.org/licenses/by/4.0
date: 2026-06-05
publication date: 2026-06-05
starting page: 798
ending page: 808
doi: 10.30605/qwqdnv69

TINJAUAN FITOKIMIA DAN AKTIVITAS ANTIDIABETES Cinnamomum zeylanicum Blume PADA DIABETES MELITUS TIPE 2 : LITERATUR REVIEW
type: item
description: Type 2 diabetes mellitus is a metabolic disorder with rapidly increasing global prevalence and a high burden of complications. Limitations of conventional therapies, including reduced efficacy and adverse effects, highlight the need for complementary approaches with multi-target actions. This study aimed to evaluate the phytochemical composition and antidiabetic potential of Cinnamomum zeylanicum as an adjunct therapy. A literature review was conducted on studies published between 2016 and 2026 retrieved from Google Scholar and ScienceDirect. The findings demonstrate that Cinnamomum zeylanicum is rich in bioactive compounds, particularly polyphenols, flavonoids, cinnamaldehyde, and eugenol, which exert glucose-lowering effects through multiple mechanisms, including inhibition of α-amylase and α-glucosidase, enhancement of insulin sensitivity, and antioxidant activity. Evidence from in vitro, in vivo, and clinical studies consistently indicates significant reductions in blood glucose levels and improvements in metabolic parameters. Overall, Cinnamomum zeylanicum shows strong potential as an effective adjunct therapeutic agent for type 2 diabetes mellitus
subject: Articles
  topic: section
subject: phytochemistry
  topic: keywords
subject: antidiabetic
  topic: keywords
subject: Cinnamomum zeylanicum
  topic: keywords
subject: type 2 diabetes mellitus
  topic: keywords
creator: Intan Putri Winanda
creator: Yuni Ahda
rights: Copyright (c) 2026 Intan Putri Winanda, Yuni Ahda https://creativecommons.org/licenses/by/4.0
date: 2026-05-16
publication date: 2026-05-16
starting page: 729
ending page: 734
doi: 10.30605/vz1wz004

LITERATUR REVIEW: APLIKASI MOLEKULER BERBASIS PCR DALAM DETEKSI DAN KARAKTERISASI Escherichia coli PADA SEKTOR LINGKUNGAN, PANGAN, DAN KLINIS
type: item
description: Escherichia coli is a gram-negative bacterium and a key indicator of water and food contamination. Conventional PCR is a widely used molecular method for specific, rapid, and sensitive E. coli detection. This literature review systematically examines the application of conventional PCR in E. coli detection across water, food, and clinical sample matrices from 2017 to 2025. Thirteen scientific articles were reviewed regarding research objects, PCR methods, research stages, and findings. Conventional PCR using primers 16E1/16E2 (584 bp), Afa FP/RP (480 bp), and ESS (825 bp) effectively detected E. coli in diverse samples. PCR showed superior sensitivity (100%) and specificity (94.34%) compared to culture methods (Hariri, 2022). Direct PCR offered a cost-effective alternative without DNA extraction (JKG, 2025). Multiplex PCR enabled simultaneous detection of multiple virulence genes (Ekawati et al.; Microbiology Spectrum, 2024). Conventional PCR remains a reliable, specific, and rapid tool for E. coli detection across diverse sample types.
subject: Articles
  topic: section
subject: Quality of service for cadres
  topic: keywords
subject: toddlers
  topic: keywords
subject: maternal visits
  topic: keywords
creator: Diva Yunavita
creator: Dwi Hilda Putri
rights: Copyright (c) 2026 Diva Yunavita, Dwi Hilda Putri https://creativecommons.org/licenses/by/4.0
date: 2026-05-11
publication date: 2026-05-11
starting page: 717
ending page: 722
doi: 10.30605/jcp1q263

DISTRIBUSI DAN KARAKTERISTIK SAMPAH WISATA DI ZONA INTERTIDAL LABUAN BAJO SERTA IMPLIKASI AWAL TERHADAP EKOSISTEM PESISIR
type: item
description: Labuan Bajo has experienced rapid tourism growth over the past decade, accompanied by increasing pressure on its coastal environments. This study aimed to examine the spatial distribution and composition of marine debris in intertidal areas and to provide an initial assessment of potential ecological implications. A descriptive survey approach was applied through field observations at selected coastal sites representing varying levels of tourism intensity. Marine debris was quantified using standardized sampling units and classified into major material categories, with further sub-classification for plastic items. The results showed that debris distribution varied across locations, with higher densities recorded in areas with intensive tourism activity. Plastic dominated the debris composition, particularly single-use packaging, plastic bags, and food-related items. Fragmented materials were also observed, indicating ongoing degradation processes that may contribute to microplastic formation. Differences in debris accumulation appeared to be influenced by local human activity and site characteristics, including accessibility and shoreline features. These findings suggest that tourism-derived debris has begun to exert measurable pressure on coastal ecosystems, especially in intertidal zones where biological interactions are frequent. While this study does not directly assess biological impacts, the observed patterns indicate potential risks related to ingestion, entanglement, and habitat disturbance. The study provides baseline information that can support future ecological monitoring and more detailed investigations in tourism-dominated coastal systems.
subject: Articles
  topic: section
subject: marine debris
  topic: keywords
subject: tourism
  topic: keywords
subject: coastal ecosystem
  topic: keywords
subject: plastic waste
  topic: keywords
subject: intertidal
  topic: keywords
subject: Labuan Bajo
  topic: keywords
creator: Muhammad Taufik Kaisupy
creator: Singgih Prasetyo Adi Wibowo
creator: Muhammad Reza Cordova
rights: Copyright (c) 2026 Muhammad Taufik Kaisupy, Singgih Prasetyo Adi Wibowo, Muhammad Reza Cordova https://creativecommons.org/licenses/by/4.0
date: 2026-05-18
publication date: 2026-05-18
starting page: 742
ending page: 752
doi: 10.30605/kw6jra20

INVENTARISASI TANAMAN HIAS DI KAMPUS UNIVERSITAS NEGERI PADANG
type: item
description: Inventarisasi tanaman hias merupakan langkah penting dalam mendukung pengelolaan ruang terbuka hijau dan penerapan konsep green campus di lingkungan perguruan tinggi. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis tanaman hias yang terdapat di Kampus Universitas Negeri Padang (UNP), mengetahui sebarannya, serta menganalisis fungsi ekologisnya. Penelitian dilaksanakan dengan menggunakan metode survei eksploratif melalui metode jelajah (cruise method). Data dikumpulkan melalui pengamatan langsung, dokumentasi, dan identifikasi tanaman berdasarkan karakter morfologi menggunakan literatur botani dan aplikasi identifikasi tanaman. Hasil penelitian menunjukkan bahwa di Kampus UNP ditemukan 12 spesies tanaman hias yang tergolong ke dalam 8 famili, dengan famili Araceae dan Asparagaceae sebagai famili yang paling dominan. Tanaman hias tersebar di berbagai area kampus seperti taman fakultas, halaman rektorat, jalur pedestrian, dan area parkir. Keberadaan tanaman hias tersebut tidak hanya berfungsi sebagai elemen estetika, tetapi juga memiliki peran ekologis penting seperti penyerap polutan udara, penghasil oksigen, pengatur suhu mikro, serta penunjang keanekaragaman hayati. Hasil inventarisasi ini diharapkan dapat menjadi data dasar dalam perencanaan dan pengelolaan lanskap hijau kampus yang berkelanjutan serta mendukung kegiatan pembelajaran dan konservasi lingkungan di Universitas Negeri Padang.
subject: Articles
  topic: section
subject: inventory
  topic: keywords
subject: ornamental plants
  topic: keywords
subject: identification
  topic: keywords
subject: campus
  topic: keywords
subject: State University of Padang for cadres
  topic: keywords
subject: toddlers
  topic: keywords
subject: maternal visits
  topic: keywords
creator: Mina Almiah
creator: Moralita Chatri
creator: Linda Advinda
creator: Filza Yulina Ade
rights: Copyright (c) 2026 Mina Almiah, Moralita Chatri, Linda Advinda, Filza Yulina Ade https://creativecommons.org/licenses/by/4.0
date: 2026-05-11
publication date: 2026-05-11
starting page: 704
ending page: 716
doi: 10.30605/yg3bpj84

Status Permudaan Alami Hutan Mangrove di Teluk Buo Bungus Kota Padang
type: item
description: Mangrove forests are important coastal ecosystems that function as shoreline protectors, carbon sinks, and habitats for many aquatic organisms. In Teluk Buo, Bungus, Padang City, mangrove stands have experienced pressure from land conversion and human activities, so information on natural regeneration is needed to support management efforts. This study aimed to determine the natural regeneration status of mangroves in Teluk Buo based on species density values. The study used a descriptive quantitative method with a plot sampling approach at three observation stations, each consisting of three plots selected by purposive sampling. Observation plots of 10 x 10 m were used for trees, 5 x 5 m for saplings, and 2 x 2 m for seedlings. Data were analyzed using species density and regeneration status criteria according to Shankar (2001). The results showed that two mangrove species were found, namely Rhizophora apiculata and Bruguiera gymnorrhiza. Station 1 had a seedling density of 75, sapling density of 12, and tree density of 26.33, indicating a sufficient regeneration status. Station 2 had a seedling density of 144, sapling density of 24, and tree density of 5.67, while Station 3 had a seedling density of 122, sapling density of 17.33, and tree density of 8.33; both stations indicated good regeneration status. Rhizophora apiculata was the dominant species at all growth levels, whereas Bruguiera gymnorrhiza showed limited regeneration. In general, natural regeneration of mangroves in Teluk Buo was classified as good to sufficient, with regeneration being more strongly supported by Rhizophora apiculata.
subject: Articles
  topic: section
subject: Mangrove
  topic: keywords
subject: natural regeneration
  topic: keywords
subject: density
  topic: keywords
subject: Rhizophora apiculate
  topic: keywords
subject: Teluk Buo
  topic: keywords
creator: Nadila Safitri
creator: Irma Leilani Eka Putri
creator: Vauzia
creator: Filza Yulina Ade
rights: Copyright (c) 2026 Nadila Safitri, Irma Leilani Eka Putri, Vauzia, Filza Yulina Ade https://creativecommons.org/licenses/by/4.0
date: 2026-05-18
publication date: 2026-05-18
starting page: 735
ending page: 741
doi: 10.30605/8kzxcf45

Literature Review: Bakteri Asam Laktat Dari Dadih Minangkabau: Keragaman, Karakteristik, Dan Potensi Aktivitas Antibakteri
type: item
description: Dadih merupakan produk susu fermentasi tradisional khas Minangkabau, Sumatera Barat, yang diproduksi melalui fermentasi spontan susu kerbau dalam tabung bambu. Produk ini telah lama dikenal sebagai sumber bakteri asam laktat (BAL) yang kaya dan beragam, dengan potensi manfaat sebagai agen probiotik, antibakteri, antioksidan, dan biopreservatif alami. Artikel review ini mengintegrasikan temuan dari berbagai studi ilmiah mengenai keragaman spesies BAL yang diisolasi dari dadih, karakteristik morfologi dan biokimianya, serta potensi aktivitas antibakterinya terhadap bakteri patogen pangan, terutama Escherichia coli dan Staphylococcus aureus. Kajian literatur menunjukkan dominasi genus Lactobacillus, Lactococcus, Pediococcus, Enterococcus, Weissella, dan Leuconostoc dalam komunitas BAL dadih. Aktivitas antibakteri BAL dadih diperantarai oleh produksi senyawa bioaktif seperti asam laktat, hidrogen peroksida, diasetil, dan bakteriosin, dengan mekanisme kerja yang melibatkan permeabilisasi membran sel dan gangguan sintesis dinding sel patogen. Isolat BAL dari dadih secara konsisten menunjukkan penghambatan lebih kuat terhadap bakteri Gram-positif (S. aureus) dibandingkan Gram-negatif (E. coli), yang dikaitkan dengan perbedaan struktur dinding sel. Dadih Minangkabau berpotensi besar sebagai sumber BAL unggulan untuk pengembangan probiotik dan biopreservatif pangan berbasis kearifan lokal
subject: Articles
  topic: section
subject: Aktivitas Antibakteri
  topic: keywords
subject: Bakteri Asam Laktat
  topic: keywords
subject: Dadih
  topic: keywords
subject: Fermentasi
  topic: keywords
subject: Probiotik
  topic: keywords
creator: Rahmi Adzra Haura
rights: Copyright (c) 2026 Rahmi Adzra Haura, Dandy Yusuf, Dwi Hilda Putri https://creativecommons.org/licenses/by/4.0
date: 2026-05-11
publication date: 2026-05-11
starting page: 723
ending page: 728
doi: 10.30605/wmgnc484

Integrasi Alat Bioinformatika Dalam Kurikulum Genetika Molekuler: Meningkatkan Kompetensi Abad 21 Mahasiswa
type: item
description: This study aims to analyze the effectiveness of bioinformatics-based molecular genetics learning in improving students’ 21st-century competencies. A quantitative approach with a pre-experimental one group pretest-posttest design was employed. The participants consisted of students from one class selected through total sampling. The research procedure included administering a pretest, implementing bioinformatics-based learning using NCBI BLAST supported by student worksheets, and conducting a posttest. Data were analyzed using descriptive statistics, N-gain, and a paired sample t-test. The results showed that the mean pretest score of 72.30 increased to 87.95 in the posttest. The N-gain value was 0.56, categorized as moderate. The paired sample t-test indicated a significant difference between pretest and posttest scores (p<0.05). These findings demonstrate that bioinformatics-based learning effectively enhances students’ 21st-century competencies, including critical thinking, problem-solving, digital literacy, and collaboration. Therefore, integrating bioinformatics into molecular genetics learning can serve as an innovative approach to improve learning quality in higher education.
subject: Articles
  topic: section
subject: Bioinformatics
  topic: keywords
subject: Molecular Genetics Learning
  topic: keywords
subject: 21st-Century Competencies
  topic: keywords
subject: N-gain
  topic: keywords
subject: Paired Sample t-test
  topic: keywords
creator: Revalina Mawarni
creator: Tomi Hidayat
rights: Copyright (c) 2026 Revalina Mawarni, Tomi Hidayat https://creativecommons.org/licenses/by/4.0
date: 2026-05-22
publication date: 2026-05-22
starting page: 753
ending page: 758
doi: 10.30605/c1egr668

INDUKSI TUNAS Musa paradisiaca L. MENGGUNAKAN KOMBINASI IBA DAN BAP SERTA AIR KELAPA
type: item
description: Pisang kepok (Musa paradisiaca L.) menjadi komoditas unggulan Indonesia yang memiliki nilai ekonomi dan gizi tinggi. Namun, perbanyakan bibit secara konvensional seringkali lambat dan rentan terhadap penyakit. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi zat pengatur tumbuh (ZPT) IBA (Indole Butyric Acid) dan BAP (Benzylaminopurine) serta penambahan air kelapa terhadap induksi tunas pisang kepok secara in vitro. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL) non-faktorial dengan empat perlakuan: A1 (MS + IBA 2 mg/L + BAP 3 mg/L), A2 (MS + IBA 3 mg/L + BAP 2 mg/L), B1 (MS + IBA 2 mg/L + BAP 3 mg/L + Air Kelapa 15%), dan B2 (MS + IBA 3 mg/L + BAP 2 mg/L + Air Kelapa 15%). Hasil penelitian menunjukkan bahwa perlakuan memberikan pengaruh nyata terhadap jumlah tunas, namun berpengaruh tidak nyata terhadap waktu muncul tunas, tinggi tunas, diameter tunas, jumlah daun, dan bobot basah eksplan. Perlakuan B1 merupakan media yang paling optimal untuk menginduksi jumlah tunas terbanyak (1,67 tunas), tinggi tunas tertinggi (3,32 cm), diameter tunas terbesar (1,42 cm), dan jumlah daun terbanyak (2,11 daun). Secara keseluruhan, kombinasi sitokinin yang lebih tinggi (BAP 3 mg/L) dibandingkan auksin (IBA 2 mg/L) didukung oleh penambahan air kelapa 15% memberikan hasil pertumbuhan lebih stabil dan optimal pada fase induksi tunas pisang kepok.
subject: Articles
  topic: section
subject: Air Kelapa
  topic: keywords
subject: BAP
  topic: keywords
subject: IBA
  topic: keywords
subject: In Vitro
  topic: keywords
subject: Pisang Kepok
  topic: keywords
creator: Nadiatul Firdha
creator: Sajaratud Dur
creator: Irda Nila Selvia
rights: Copyright (c) 2026 Nadiatul Firdha, Sajaratud Dur, Irda Nila Selvia https://creativecommons.org/licenses/by/4.0
date: 2026-05-03
publication date: 2026-05-03
starting page: 666
ending page: 673
doi: 10.30605/qqtxmd98

LITERATURE REVIEW: KARAKTERISTIK DAN DISTRIBUSI CORONAVIRUS PADA KELELAWAR SEBAGAI RESERVOIR ALAMI
type: item
description: Coronavirus merupakan virus RNA untai tunggal positif yang memiliki keragaman genetik tinggi dan mampu berevolusi melalui mutasi serta rekombinasi. Kelelawar diketahui sebagai reservoir alami coronavirus karena memiliki sistem imun yang unik serta karakteristik ekologi yang mendukung penyebaran virus. Penelitian ini bertujuan untuk menganalisis dan mensintesis berbagai hasil penelitian mengenai karakteristik dan distribusi coronavirus pada kelelawar. Metode yang digunakan adalah literature review dengan mengumpulkan artikel dari Google Scholar, PubMed, dan ScienceDirect dalam sepuluh tahun terakhir. Hasil kajian menunjukkan bahwa coronavirus pada kelelawar memiliki keragaman genetik yang tinggi, terutama pada kelompok Alphacoronavirus dan Betacoronavirus, serta tersebar luas di berbagai wilayah, khususnya Asia Tenggara. Beberapa penelitian juga menunjukkan adanya potensi transmisi lintas spesies (spillover) yang dipengaruhi oleh adaptasi virus dan faktor lingkungan. Kesimpulannya, kelelawar berperan penting dalam menjaga keragaman dan distribusi coronavirus, sehingga diperlukan pemantauan yang berkelanjutan untuk mencegah munculnya penyakit zoonotik di masa depan.
subject: Articles
  topic: section
subject: coronavirus kelelawar
  topic: keywords
subject: zoonosis
  topic: keywords
subject: keragaman genetik
  topic: keywords
subject: distribusi
  topic: keywords
subject: reservoir
  topic: keywords
creator: Desrinda Dwi Adinda Putri
creator: Sugiyono Saputra
creator: Dwi Hilda Putri
rights: Copyright (c) 2026 Desrinda Dwi Adinda Putri, Sugiyono Saputra, Dwi Hilda Putri https://creativecommons.org/licenses/by/4.0
date: 2026-04-29
publication date: 2026-04-29
starting page: 652
ending page: 659
doi: 10.30605/azkxbc74

JENIS-JENIS TANAMAN HORTIKULTURA DI DESA SINDANG KASIH KECAMATAN RANOMEETO BARAT KABUPAEN KONAWE SELATAN
type: item
description: Horticulture is a field of agricultural science that focuses on the cultivation of fruits, vegetables, and ornamental plants. The term is derived from the Latin words "hortus," meaning garden, and "colere," meaning cultivation. Horticulture literally refers to the cultivation of these types of plants. However, in the 1993-1998 State Policy Guidelines (GBHN), the horticulture group was expanded to include medicinal plants, in addition to fruits, vegetables, and ornamental plants. Plant identification is a discipline within botany that focuses on the process of recognizing and classifying plants based on morphological, anatomical, physiological, ecological and molecular characteristics. This research aims to identify the types of horticultural plants in Sindang Kasih Village, Ranomeeto Barat District, South Konawe Regency, Southeast Sulawesi. This study uses a qualitative descriptive method with an exploratory technique. The data analysis technique used in this study is descriptive. The results of the study found 23 types of vegetable horticultural plantst consisting of 12 families including Amaranthaceae, Amaryllidaceae, Apiaceae, Asteraceae, Brassicaceae, Convolvulaceae, Euphorbiaceae, Fabaceae, Lamiaceae, Malvaceae, Moringaceae, and Solanaceae. In fruit horticultural plants, 32 types were found consisting of 22 families including Anacardiaceae, Annonaceae, Arecaceae, Bromeliaceae, Cactaceae, Caricaceae, Clusiaceae, Cucurbitaceae, Meliaceae, Moraceae, Muntingiaceae, Musaceae, Myrtaceae, Oxalidaceae, Passifloraceae, Poaceae, Rubiaceae, Rutaceae, Sapindaceae, Sapotaceae, Sterculiaceae, and Vitaceae.
subject: Articles
  topic: section
subject: Types
  topic: keywords
subject: Horticultural Plants
  topic: keywords
subject: Vegetables
  topic: keywords
subject: Fruits
  topic: keywords
subject: Sindang Kasih Village
  topic: keywords
creator: Damhuri
creator: Asmawati Munir
creator: Cheryl Artha Agnestesyah Berlin
rights: Copyright (c) 2026 Damhuri, Asmawati Munir, Cheryl Artha Agnestesyah Berlin https://creativecommons.org/licenses/by/4.0
date: 2026-05-24
publication date: 2026-05-24
starting page: 764
ending page: 772
doi: 10.30605/hftm1326

Analisis Keterampilan Metakognitif Siswa Kelas VIII SMP Makassar Raya dalam Menjawab Soal Berbasis HOTS
type: item
description: This study aimed to analyze the metacognitive skills of eighth-grade junior high school students in Makassar Raya in answering Higher Order Thinking Skills (HOTS)-based questions. The study employed a quantitative approach with a descriptive-analytical design. The participants were 27 students selected through total sampling. Data were collected through a HOTS-based essay test consisting of 10 items. Each student response was assessed using the Corebima (2008) metacognitive skills rubric, with a score range of 0–7. The data were analyzed using descriptive statistics, including frequency, percentage, mean, median, mode, and standard deviation. The results showed that the students’ metacognitive skills were dominated by a score of 4, achieved by 12 students (44.44%). A score of 3 was obtained by 7 students (25.93%), a score of 5 by 3 students (11.11%), scores of 2 and 6 by 2 students each (7.41%), and a score of 7 by 1 student (3.70%). No students obtained a score of 1. These findings indicate that the students’ metacognitive skills were in the moderate or transitional category. In general, the students were able to provide correct, coherent, logical, and well-reasoned answers, but they had not yet optimally expressed their responses in their own words. Therefore, HOTS-based instruction needs to be accompanied by strategies that encourage students to plan, monitor, and evaluate their thinking processes more reflectively.
subject: Articles
  topic: section
subject: Metacognitif Skills
  topic: keywords
subject: HOTS
  topic: keywords
subject: Science Learning
  topic: keywords
creator: Irwandi Rahmat
rights: Copyright (c) 2026 Irwandi Rahmat https://creativecommons.org/licenses/by/4.0
date: 2026-05-09
publication date: 2026-05-09
starting page: 692
ending page: 697
doi: 10.30605/2ne0yv49

Efek Seed Priming KNO3 terhadap Perkecambahan dan Pertumbuhan Awal Benih Padi Varietas IR 64 pada Beberapa Tingkat Salinitas
type: item
description: Salinity is one of the major limiting factors in rice cultivation, negatively affecting seed germination and early plant growth. One approach that can be taken to improve seed tolerance under saline conditions is seed priming. This study aims to examine the effect of potassium nitrate (KNO3) seed priming on the germination and early growth of IR 64 rice seeds at various salinity levels. This study was an experiment designed using a completely randomized design with a single treatment factor, namely NaCl salinity levels (0, 50, 100, and 150 mM), in which all seeds were treated with 1% KNO3 seed priming prior to stress application. Each treatment was replicated four times. The observed parameters included germination percentage, root length, shoot length, seedling length, and seed vigor index. The data were analyzed using analysis of variance (ANOVA) followed by Duncan’s multiple range test at a 5% significance level. The results showed that KNO3 seed priming was able to maintain the germination percentage of IR 64 seeds at all salinity levels, with values that were not statistically significantly different. However, increasing NaCl concentrations had a significant negative effect on the morphological growth of seedlings, as indicated by a decrease in root length, shoot length, seedling length, and seed vigor index. The treatment without salinity produced the best early growth, while high salinity significantly inhibited seedling growth. Based on these results, it can be concluded that KNO3 seed priming plays an effective role in maintaining the viability of IR 64 rice seeds under saline conditions, but it is not yet fully capable of overcoming the negative effects of salinity on early plant growth.
subject: Articles
  topic: section
subject: Seed priming
  topic: keywords
subject: KNO3
  topic: keywords
subject: salinity
  topic: keywords
subject: IR 64 rice
  topic: keywords
subject: germination
  topic: keywords
creator: Puji Ramadhani
creator: Annisa Mardhatillah
creator: Violita Violita
rights: Copyright (c) 2026 Puji Ramadhani, Annisa Mardhatillah, Violita Violita https://creativecommons.org/licenses/by/4.0
date: 2026-04-29
publication date: 2026-04-29
starting page: 660
ending page: 665
doi: 10.30605/hcqyq463