Jurnal Biogenerasi menyajikan artikel orisinal tentang pengetahuan dan informasi riset atau aplikasi riset dan pengembangan dalam bidang biologi dan pendidikan biologi. Bidang kajian Jurnal Biogenerasi meliputi hasil penelitian, uraian metode dan teknik inovasi penelitian serta ulasan/ tinjauan ilmiah mutakhir hasil penelitian dalam bidang di bidang Biologi, edukasi serta pengembangan IPTEK.
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Analisis Produksi Biolistrik oleh Aneurinibacillus thermoaerophilus Strain N25 pada Variasi Konsentrasi Starter dalam Sistem Microbial Fuel Cell (MFC)
The increasing demand for electrical energy drives the development of renewable energy technologies such as Microbial Fuel Cell (MFC), which converts organic substrates into electricity using microorganisms. This study aims to determine the effect of starter concentration and identify the optimal concentration of Aneurinibacillus thermoaerophilus strain N25 in bioelectricity production. An experimental method was used with a dual-chamber MFC system and starter concentrations of 3%, 6%, 9%, 12%, and 15%. Voltage production was measured using a digital multimeter (V) over 24 hours. The results showed that starter concentration affected bioelectricity production, with voltage increasing over time and reaching a peak at the 20th hour. Higher starter concentrations produced higher voltage due to increased microbial activity and more efficient electron transfer.
Copyright (c) 2026 Echy Dania Putri, Irdawati, Azwir Anhar, Moralita Chatri https://creativecommons.org/licenses/by/4.0
POLA PRODUKSI BIOLISTRIK Aneurinibacillus thermoaerophilus STRAIN N25 PADA VARIASI SUBSTRAT LIMBAH PERTANIAN
The increasing energy demand encourages the development of renewable energy technologies such as Microbial Fuel Cell (MFC), which converts organic substrates into electricity using microorganisms. This study aimed to analyze bioelectricity production by Aneurinibacillus thermoaerophilus strain N25 using different substrates (glucose, corn cob, rice straw, and rice husk). The experiment was conducted using a dual-chamber MFC system with voltage measurements over 24 hours. The results showed that voltage increased over time, peaked at the 20th hour, and then decreased. The highest voltage was produced by glucose (0.802 mV), followed by corn cob (0.724 mV), rice straw (0.581 mV), and rice husk (0.491 mV). These results indicate that substrate type affects bioelectricity production, with corn cob as the best agricultural waste substrate.
Copyright (c) 2026 Dinda Aulia Nabila, Irdawati, Dezi Handayani, Elsa Yuniarti https://creativecommons.org/licenses/by/4.0
POLA PRODUKSI LISTRIK Bacillus licheniformis strain KGEB16 AMOBIL PADA SISTEM MICROBIAL FUEL CELL (MFC)
Peningkatan kebutuhan energi berkelanjutan telah menunutut pengembangan Microbial Fuel Cell (MFC) sebagai teknologi alternatif. Penelitian ini bertujuan untuk menganalisis pola produksi listrik Bacillus licheniformis strain KGEB16 yang diamobilisasi pada sistem Microbial Fuel Cell (MFC). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan variasi konsentrasi natrium alginat (2%, 3%, 4%, 5%, dan 6%) serta lima ulangan. Proses amobilisasi sel dilakukan dengan metode entrapment menggunakan natrium alginat, sedangkan pengukuran tegangan listrik dilakukan setiap 2 jam selama periode inkubasi 24 jam. Data yang diperoleh dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Duncan (Duncan Multiple Range Test/DMRT) pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa pola produksi listrik mengalami peningkatan secara bertahap sejak awal inkubasi hingga mencapai puncak pada jam ke-20, kemudian menurun pada jam ke-22 hingga 24. Tegangan tertinggi diperoleh pada konsentrasi natrium alginat 5%, sedangkan tegangan terendah diperoleh pada konsentrasi 2%. Dengan demikian, dapat disimpulkan bahwa konsentrasi natrium alginat berpengaruh signifikan terhadap pola produksi listrik, dengan konsentrasi optimum sebesar 5% pada sistem MFC.
Copyright (c) 2026 fazdkia oktriani, Irdawati, Siska Alicia Farma, Fadilaturahmah https://creativecommons.org/licenses/by/4.0
PENGARUH ISOMETRIC HANDGRIP EXERCISE TERHADAP PERUBAHAN TEKANAN DARAH PADA LANSIA PENDERITA HIPERTENSI
Hypertension or high blood pressure is one of the main health problems in the elderly that risks reducing the quality of life and increasing morbidity and mortality. Hypertension management efforts can be done pharmacologically and non-pharmacologically, one of which is by doing isometric handgrip exercise. This study aims to determine the effect of isometric handgrip exercise and its frequency on changes in blood pressure in elderly people with hypertension in X Village, Madiun Regency. This type of research uses a pre-experimental design with a one-group pretest-posttest design approach. The population of this study was elderly people with hypertension in X Village, Madiun Regency. The sample amounted to 18 respondents selected using a simple random sampling technique. Data were collected by measuring blood pressure before and after being given isometric handgrip exercise intervention for 5 consecutive days, then analyzed using the Wilcoxon signed rank test. The results showed that the average systolic blood pressure before the intervention was 151.67 mmHg and after the intervention became 133.33 mmHg, while the average diastolic blood pressure before the intervention was 91.67 mmHg and after the intervention became 77.78 mmHg. The results of the Wilcoxon test obtained a ρ-value = 0.000 (p < 0.05), which means there is a significant effect of isometric handgrip exercise on changes in blood pressure in elderly people with hypertension. This study shows that isometric handgrip exercise is effective in lowering blood pressure in elderly people with hypertension. This exercise can be used as a simple, safe, inexpensive, and easy to do non-pharmacological intervention independently at home.
Copyright (c) 2026 Hariyadi, Elen Ayu Ananda Prianita, Rela Hastuti, Sri Suhartiningsih https://creativecommons.org/licenses/by/4.0
POTENSI Bacillus licheniformis strain Nam 2-2 DALAM PRODUKSI BIOLISTRIK PADA VARIASI pH
The increasing global energy demand has encouraged the development of sustainable alternative energy sources such as Microbial Fuel Cells (MFCs). This study aimed to determine the optimum pH for bioelectricity production using Bacillus licheniformis strain Nam 2-2 in a dual-chamber MFC system. The experiment employed a completely randomized design with pH variations of 7, 7.5, and 8. Voltage output was measured every 2 hours over a 24-hour incubation period. The results showed that bioelectricity production increased over time, reached a peak at 16–20 hours, and then declined. The highest voltage was observed at pH 7.5, reaching 0.782 mV, followed by pH 8 (0.758 mV) and pH 7 (0.748 mV). These findings indicate that slightly alkaline conditions are more favorable for microbial activity and electron transfer efficiency. Therefore, pH optimization is essential to improve MFC performance and supports its potential as a renewable energy source.
Copyright (c) 2026 Silvi Tamara Silvi, Irdawati, Linda Advinda, Elsa Yuniarti https://creativecommons.org/licenses/by/4.0
Optimasi Primer : Suhu Annealing untuk Deteksi Gen Matrix Virus Influenza A Menggunakan Polymerase Chain Reaction (PCR)
Influenza A virus (IAV) is a pathogen that attacks the respiratory tract in humans and animals, which can be a threat to public health. This virus has a number of genes that are targeted by PCR for diagnostic detection purposes, one of which is the matrix gene. Detection of the matrix gene in the Influenza A virus from DNA/RNA genetic material can be done using the polymerase chain reaction (PCR) technique. One of the stages that determines the success of the PCR process is the annealing stage, for which the optimum temperature and cycle are required for primer attachment in the annealing stage. This research is a descriptive study towards an experiment conducted in June 2025 at the Bacteriology Laboratory of the Research and Innovation Agency (BRIN) KST. Soekarno Cibinong. The results obtained showed that the optimum temperature for matrix gene amplification in the Influenza A virus using the forward primer FluAV M-U44 and reverse primer FluAV M-L287 was 53.5 °C, 54 °C, and 54,5 °C and 35 cycles were evidenced by the formation of a fairly bright band in the electrophoresis results.
Copyright (c) 2026 Natalia Turnip, Nova Dilla Yanthi, Dwi Hilda Putri https://creativecommons.org/licenses/by/4.0
HUBUNGAN SIKAP IBU DENGAN KEJADIAN UNDERWEIGHT PADA BALITA DI WILAYAH KERJA PUSKESMAS COT SEUMEURENG
Good nutritional status in toddlers is essential for child health and development. Nationally, the prevalence of underweight among children under five in Indonesia is 15.9%, consisting of 3.0% severely underweight and 12.9% underweight, while 84.1% of toddlers have normal nutritional status. At the provincial level, Aceh shows a higher prevalence of underweight at 22.6%, including 4.9% severely underweight, 17.7% underweight, and 77.4% with normal nutritional status. At the district level, data from the Aceh Barat Health Office in 2024 recorded 530 cases of underweight, with the highest distribution in the working areas of Cot Seumeureung Health Center (85 cases), Kuala Bhee Health Center (84 cases), and Johan Pahlawan Health Center (83 cases).This condition highlights the importance of the mother’s role, particularly maternal attitudes toward fulfilling toddlers’ nutritional needs. This study aimed to analyze the relationship between maternal attitudes and the incidence of underweight among toddlers in the working area of Cot Seumeureung Health Center. A quantitative cross-sectional design was used, involving 63 mothers of children aged 0–59 months from three villages through total sampling. Data were collected from November to December 2025 using questionnaires, interviews, and anthropometric measurements based on WHO weight-for-age (WFA) z-score standards, and analyzed using the chi-square test (α = 0.05). The results showed that most mothers had positive attitudes toward toddler nutrition, and there was a significant relationship between maternal attitudes and the incidence of underweight (p = 0.040). In conclusion, maternal attitudes play an important role in determining toddlers’ nutritional status; therefore, underweight prevention efforts should focus on fostering positive attitudes, appropriate feeding practices, and strengthening continuous nutrition education and assistance through integrated health posts (posyandu) and health workers.
Copyright (c) 2026 Muriza Rahmatul Husna, Sri Wahyuni Muhsin, Ravi Masitah, Cukri Rahma https://creativecommons.org/licenses/by/4.0
Penerapan Prinsip Bioetik dalam Pengujian Escherichia coli pada Air dan Salmonella pada Ikan Nila (Oreochromis niloticus) Menggunakan Media Compact Dry Berdasarkan Standar ISO/IEC 17025 di Laboratorium BPPMHKP Medan I
Food safety and environmental quality are critical aspects in maintaining public health, particularly in fishery products and water sources that are vulnerable to contamination by pathogenic microorganisms. This study aims to identify the presence of Escherichia coli in water samples and Salmonella in tilapia (Oreochromis niloticus), as well as to analyze the use of Compact Dry media based on ISO/IEC 17025 and the implementation of bioethical principles in microbiological testing. This research employed a descriptive laboratory approach conducted at the BPPMHKP Medan I Laboratory using Compact Dry media based on AOAC methods. The samples consisted of lake water and tilapia, which were tested through enrichment, inoculation, incubation, and colony observation stages. The results showed no growth of Escherichia coli colonies on Compact Dry EC media and no Salmonella colonies on Compact Dry SL media, indicating that all samples were negative for the tested bacteria. The use of Compact Dry media in this study complied with ISO/IEC 17025 standards, as indicated by systematic procedures, the use of validated media, and laboratory quality control. Furthermore, bioethical principles, including scientific integrity, responsibility, biosafety, and accountability, were applied throughout all stages of testing. Therefore, this study demonstrates that the use of validated media and the application of bioethical principles play an important role in ensuring valid, reliable, and accountable laboratory results.
Copyright (c) 2026 Andina Ayu Syahputri, Dini Dara Arifia, Nurbaity Situmorang, Mhd Yusuf Nasution, Widya Arwita https://creativecommons.org/licenses/by/4.0
Kode Etik Pengelolaan Limbah Bekas Dan Bahan Terkontaminasi Pada Laboratorium Kultur Jaringan Tanaman
This study aims to describe the management of used culture media waste and contaminated materials, and to assess the understanding of laboratory staff and interns regarding ethical principles in plant tissue culture laboratories. A descriptive qualitative method was applied using observation, questionnaires, and literature review. The results indicate that most respondents have a good understanding, reflected in their agreement on the importance of sterilization, waste segregation, use of personal protective equipment, and implementation of standard operating procedures (SOP). Respondents are also aware of the risks of laboratory waste to health and the environment. Ethical practices are recognized as part of professional responsibility, but require improvement through training, supervision, and consistent SOP implementation.
Copyright (c) 2026 Najwa Athifah Athifah, Ribi Zuhriah Sani Sani, Mhd Yusuf Nasution, Khairiza Lubis, Nurbaity Situmorang https://creativecommons.org/licenses/by/4.0
ANALISIS PENERAPAN PRINSIP BIOETIK PADA PENGELOLAAN LIMBAH B3 DILABORATORIUM KESEHATAN DAERAH KOTA BEKAS
The management of hazardous and toxic waste (B3) in health laboratories remains a challenge, particularly in ensuring regulatory compliance while consistently applying bioethical principles. This issue is important because improper waste management can negatively impact the environment and public health. Therefore, this study aims to analyze the B3 waste management system, its compliance with regulations, and the implementation of bioethical principles at the Regional Health Laboratory (Labkesda) of Bekasi City. This study employed a descriptive qualitative method with a case study approach. Data were collected through interviews, observations, and documentation involving two informants directly engaged in waste management. In addition, the implementation of bioethics was assessed using a Likert scale with ten indicators. The results show that B3 waste management has been carried out in a structured manner, including identification, containment, transportation, temporary storage, and processing by licensed third parties, thus complying with applicable regulations. Furthermore, the implementation of bioethical principles achieved an average score of 4.8 out of 5, indicating a very good category. However, an obstacle was identified in the form of delays in waste collection by third parties. In conclusion, Labkesda Bekasi City has been able to manage B3 waste effectively and sustainably, although improvements in external coordination are still needed
Keywords: hazardous waste; bioethics; health laboratory; waste management; regulation; Labkes
Copyright (c) 2026 NAUFAL, ELSHA, Nurbaity Situmorang, Khairiza Lubis https://creativecommons.org/licenses/by/4.0
Penerapan Model Pembelajaran Cooperative Learning Tipe Make A Match Untuk Meningkatkan Hasil Belajar Siswa Kelas VIIB SMPN 13 Pontianak
The study aimed to improve student learning outcomes through the implementation of the cooperative learning model of the make a match type. This research was a classroom action study conducted in two cycles, each consisting of planning, action, observation, and reflection stages. The findings show an improvement in the implementation of the learning process, increasing from 97.05% in the first cycle to 100% in the second. In addition, student learning mastery improved from 76.19% to 85.71%. Based on these results, it can be concluded that the cooperative learning model of the make a match type effectively enhances student learning outcomes in the subtopic of interactions among components of an ecosystem.
Copyright (c) 2026 Rosi Desfitri, Titin, Andi Besse Tenriawaru https://creativecommons.org/licenses/by/4.0
PENGARUH PEMANFAATAN LIMBAH CANGKANG TELUR AYAM SEBAGAI BIOFERTILIZER TERHADAP PERTUMBUHAN KACANG TANAH (Arachis hypogaea L.)
This research explores the potential of chicken eggshell waste as an alternative biofertilizer to reduce dependence on synthetic chemical fertilizers, which often trigger land degradation. The main focus of this study was to analyze the extent to which eggshell powder application affects the vegetative phase of peanut (Arachis hypogaea L.) plants, while also determining the most responsive application dose. The experiment was conducted with a four-stage treatment design: P0 (control), P1 (133.3 g/polybag), P2 (200 g/polybag), and P3 (266.6 g/polybag). Parameters observed included plant height dynamics and leaf productivity. The results showed that the integration of eggshell biofertilizer significantly stimulated plant growth compared to the control. The P3 dose (266.6 g/polybag) was identified as the most effective treatment, with an average plant height of 26 cm and 32 leaves at the end of the observation period. Interestingly, at the intermediate dose (P2), there was evidence of inhibition of stem elongation despite the high leaf count. This phenomenon is thought to be related to nutritional imbalances caused by excess calcium (Ca), which inhibits the absorption of other essential micronutrients. Overall, this study confirms that the use of eggshell waste not only serves as a natural source of calcium carbonate (CaCO₃) for soil pH stabilization but is also effective in sustainably improving the agronomic performance of peanuts.
Copyright (c) 2026 Febi Permata Jingga, Azwir Anhar https://creativecommons.org/licenses/by/4.0
Penerapan Prinsip Bioetik dalam SOP Pengendalian Hama Oryctes rhinoceros pada Perkebunan Kelapa Sawit
Kelapa sawit merupakan salah satu komoditas perkebunan yang memiliki peranan penting dalam perekonomian Indonesia. Namun, produktivitas tanaman kelapa sawit sering terganggu oleh serangan hama, salah satunya adalah Oryctes rhinoceros yang dikenal sebagai kumbang tanduk. Penelitian ini bertujuan untuk menganalisis penerapan prinsip bioetik dalam Standar Operasional Prosedur (SOP) pengendalian hama Oryctes rhinoceros pada perkebunan kelapa sawit di PT. Socfin Indonesia Kebun Bangun Bandar. Penelitian ini menggunakan pendekatan kualitatif dengan metode observasi langsung dan wawancara terhadap pekerja di lapangan. Hasil penelitian menunjukkan bahwa penerapan SOP pengendalian hama telah dilaksanakan dengan baik melalui penggunaan alat pelindung diri serta prosedur penyemprotan yang terstruktur. Penerapan tersebut mencerminkan prinsip bioetik terutama dalam aspek keselamatan kerja dan tanggung jawab terhadap lingkungan. Namun demikian, pemahaman pekerja terhadap konsep bioetik masih terbatas karena sebagian besar pekerja hanya memahami SOP sebagai aturan kerja. Oleh karena itu, diperlukan peningkatan sosialisasi dan pelatihan terkait bioetik agar penerapan SOP dapat dilakukan secara lebih optimal dan berkelanjutan.
Copyright (c) 2026 Celine Sitinjak, Farina Justin Sihombing, Nurbaity Situmorang, Mhd Yusuf Nasution https://creativecommons.org/licenses/by/4.0
ETIKA PELEPASLIARAN SATWA HASIL REHABILITASI OLEH LEMBAGA KONSERVASI OIC: ANTARA KESEJAHTERAAN INDIVIDU DAN KEBERLANJUTAN POPULASI
Konflik antara manusia dan orangutan menjadi tantangan dalam upaya konservasi, sehingga diperlukan proses rehabilitasi dan pelepasliaran satwa ke habitat alaminya. Penelitian ini bertujuan untuk mengkaji aspek bioetika dalam pelepasliaran satwa hasil rehabilitasi, khususnya dalam menyeimbangkan kesejahteraan individu dan keberlanjutan populasi. Metode yang digunakan adalah deskriptif melalui kajian literatur dan analisis proses rehabilitasi serta pelepasliaran oleh lembaga konservasi. Hasil menunjukkan bahwa pelepasliaran meliputi beberapa tahapan penting, yaitu pemeriksaan kondisi satwa, transportasi, dan pelepasan ke habitat alami. Keberhasilan pelepasliaran dipengaruhi oleh kesiapan fisik dan perilaku satwa, serta kondisi habitat rilis. Secara bioetika, keputusan pelepasliaran perlu mempertimbangkan kesejahteraan individu tanpa mengabaikan stabilitas populasi liar. Dengan demikian, pelepasliaran satwa harus dilakukan secara terencana dan berbasis pertimbangan ilmiah agar mendukung keberlanjutan ekosistem.
Copyright (c) 2026 Difa Dwi Putri, Dinda Legawa, Putri Cahaya Hadisti, Mhd Yusuf Nasution, Widya Arwita https://creativecommons.org/licenses/by/4.0
ANALISIS PRINSIP BIOETIK DALAM PENANGANAN AWAL SATWA BURUNG HASIL PENANGKAPAN DI PUSAT PENYELAMATAN SATWA TWA SIBOLANGIT
Penangkapan burung liar di Indonesia menjadi ancaman besar bagi kelestarian spesies dan kesejahteraan hewan. Pusat Pelestarian Satwa (PPS) memiliki peranan vital dalam mengelola burung yang disita melalui serangkaian proses awal, yang mencakup penerimaan, pemeriksaan kesehatan, dan karantina sebelum rehabilitasi. Tujuan penelitian ini adalah untuk menilai pelaksanaan prinsip bioetika dalam penanganan awal burung di PPS serta mengukur hubungannya dengan kesejahteraan hewan. Metode yang dipakai adalah pendekatan kualitatif deskriptif melalui observasi langsung terhadap penerimaan burung di PPS Sibolangit pada bulan Januari 2026. Temuan penelitian menunjukkan bahwa proses penanganan, termasuk pencatatan administratif, pemeriksaan kesehatan awal, serta penempatan dalam kandang karantina, telah dilakukan secara terstruktur. Pemeriksaan fisik dan observasi perilaku membantu petugas dalam menentukan kondisi burung serta memilih metode penanganan yang tepat. Prinsip bioetika seperti manfaat, tidak merugikan, dan keadilan juga telah diterapkan, yang terlihat dari pemberian perawatan yang tepat, penanganan yang berhati-hati, serta perlakuan yang adil terhadap semua burung. Secara keseluruhan, penanganan awal di PPS telah mendukung peningkatan kesejahteraan hewan.
bioetik, kesejahteraan hewan, burung, pusat perlindungan satwa, penanganan awal
Copyright (c) 2026 Alya Zakia Azzahra Nasution, Najwa Al Mukarromah, Mhd. Yusuf Nasution, Khairiza Lubis, Widya Arwita https://creativecommons.org/licenses/by/4.0
POTENSI ANTIBAKTERI TANAMAN PARE HUTAN (Momordica balsmina L.) TERHADAP BAKTERI Staphylococcus aureus
The increasing number of multidrug-resistant (MDR) bacteria due to the widespread use of antibiotics has caused health problems, especially in healthcare facilities, one of which is Staphylococcus aureus bacteria. The purpose of this study was to examine the potential of Bitter Melon (Momordica balsmina L.) leaves as an antibacterial against S. aureus bacteria. This study included a type of laboratory experimental research using a maceration extraction method with 96% ethanol solvent, while the antibacterial test used a well diffusion method. The results of the study on the potential of M. balsmina leaf extract as an antibacterial against S. aureus bacteria obtained an inhibition zone diameter of 11.3 mm and was categorized as strong. The findings in this study indicate that the bitter melon plant has the potential as a source of antibacterial agents, so further investigation is needed.
Copyright (c) 2026 Slamet Ifandi, Andi Lindhemutianingrum Siradje, Nur Fatiyah Alhabsyie, Nathasya Anggraini Kabo, Rani Salsabila https://creativecommons.org/licenses/by/4.0
EVALUASI PENERAPAN PRINSIP BIOETIKA DALAM KESEJAHTERAAN HEWAN (ANIMAL WELFARE) BERDASARKAN FIVE FREEDOMS PADA SATWA DI PUSAT PENYELAMATAN SATWA TAMAN WISATA ALAM SIBOLANGIT
Penelitian ini bertujuan untuk mengevaluasi penerapan prinsip bioetika dalam kesejahteraan hewan (animal welfare) berdasarkan konsep Five Freedoms pada satwa di Pusat Penyelamatan Satwa (PPS) Taman Wisata Alam Sibolangit. Metode yang digunakan adalah deskriptif dengan pendekatan kuantitatif melalui observasi langsung dan dokumentasi. Penilaian dilakukan terhadap beberapa indikator, yaitu ketersediaan pakan, kebersihan kandang, kondisi kesehatan satwa, ukuran kandang, dan pengamatan perilaku satwa. Hasil penelitian menunjukkan bahwa pengelolaan satwa di PPS TWA Sibolangit telah memenuhi sebagian besar prinsip Five Freedoms, meliputi bebas dari lapar dan haus, bebas dari ketidaknyamanan, bebas dari penyakit, bebas mengekspresikan perilaku alami, serta bebas dari stres. Nilai kesejahteraan satwa diperoleh sebesar 92% yang termasuk dalam kategori sangat baik. Secara bioetika, pengelolaan satwa telah mencerminkan prinsip beneficence dan non-maleficence. Namun demikian, masih diperlukan peningkatan pada aspek ukuran kandang, pengayaan lingkungan, dan frekuensi pemberian pakan agar kesejahteraan satwa dapat lebih optimal.
Copyright (c) 2026 Nabila Syahfitri, Nazwa Syafitri, Muhammad Yusuf, Khairiza Lubis, Widya Arwita https://creativecommons.org/licenses/by/4.0
KERAGAMAN JAMUR MAKROSKOPIS DI SEKITAR TITIK PEMASANGAN KAMERA TRAP DI KAWASAN HUTAN NAGARI PARIAN LUNANG, PESISIR SELATAN
Indonesia is a tropical country with a high level of biodiversity, including the diversity of macrofungi which play an important role as decomposers in the process of organic matter decomposition and in maintaining ecosystem balance. The Nagari Parian Lunang Forest Area, Pesisir Selatan, West Sumatra, has humid and relatively natural environmental conditions that potentially support the growth of various species of macrofungi. This study aims to identify the species of macrofungi, determine their potential uses, and analyze the influence of environmental factors on their distribution patterns. This research was a descriptive study conducted from September to December 2025 using an exploration method at several camera trap installation points. The mushrooms found were recorded based on their morphological characteristics, documented, and identified using the Collins Fungi Guide and related scientific literature. Environmental parameters measured included light intensity, soil temperature, and air humidity. The results showed that 28 species of macrofungi were successfully identified. Several species have potential uses as food sources, traditional medicine, and producers of bioactive compounds such as antibiotics and antitumor agents. The results of the Canonical Correspondence Analysis (CCA) indicated that light intensity, soil temperature, and air humidity influence the distribution patterns of mushrooms at each observation point.
Copyright (c) 2026 Fichi Azra Syaqila, Dezi Handayani https://creativecommons.org/licenses/by/4.0
Pengaruh Dosis Pupuk Organik Cair FloraOne® Terhadap Pertumbuhan Tanaman Tomat (Solanum lycopersicum L.)
Tomato plants (Solanum lycopersicum L.) are horticultural commodities that have high nutritional value and economic value and are widely needed by the community. The increase in demand for tomatoes needs to be balanced with efforts to increase production through proper cultivation techniques, one of which is through fertilization. The continuous use of inorganic fertilizers can reduce soil fertility and negatively impact the environment, so a more environmentally friendly fertilization alternative, such as the use of liquid organic fertilizer (POC), is needed. FloraOne® liquid organic fertilizer contains nutrients and microorganisms that have the potential to support plant growth. This study aims to determine the effect of applying FloraOne® liquid organic fertilizer on the growth of tomato plants. The research was carried out using an experimental method with a Complete Random Design (RAL) consisting of seven FloraOne® concentration treatments, namely P0 (control), P1 (0.1%), P2 (0.2%), P3 (0.3%), P4 (0.4%), P5 (0.5%), and P6 (0.6%) with four replicates. The observed parameters included plant height, stem diameter, wet weight, dry weight, and flowering age. The data was analyzed using the Analysis of Variance (ANOVA) test at a significance level of 5%. The results showed that the application of FloraOne® liquid organic fertilizer did not have a significant effect on the height, diameter, wet and dry weight of tomato plants (p>0.05) while P3 and P6 showed the appearance of flowers in the 8th week. However, descriptively, the 0.3% concentration treatment showed a better growth tendency than the other treatments.
Copyright (c) 2026 Hoki Masriono Manik, Azwir Anhar https://creativecommons.org/licenses/by/4.0
KAJIAN LITERATUR MIKROSTRUKTUR SISIK IKAN DALAM STUDI TAKSONOMI IKAN
Taksonomi ikan umumnya menggunakan pendekatan morfometri dan meristik dalam proses identifikasi spesies, namun metode tersebut memiliki keterbatasan pada spesies yang berkerabat dekat dan memiliki kemiripan morfologi eksternal. Kajian ini bertujuan menelaah peran mikrostruktur sisik sebagai karakter taksonomi pendukung dalam studi identifikasi ikan. Penelitian dilakukan melalui metode systematic literature review terhadap berbagai publikasi ilmiah yang membahas analisis mikrostruktur sisik menggunakan mikroskop cahaya dan scanning electron microscope (SEM). Hasil kajian menunjukkan bahwa komponen mikrostruktur seperti fokus, circuli, radii, lepidont, tubercle, ctenii, serta struktur canal lateral memiliki variasi yang konsisten antarspesies maupun antar lokasi sisik pada tubuh ikan. Perbedaan karakter tersebut terbukti mampu membedakan spesies yang secara morfologi makroskopis sulit dipisahkan, serta sejalan dengan data kekerabatan molekuler. Selain pada ikan modern, mikrostruktur sisik fosil juga dapat digunakan untuk interpretasi paleoekologi dan estimasi umur. Dengan demikian, analisis mikrostruktur sisik berpotensi menjadi karakter taksonomi pelengkap yang penting dalam memperkuat sistem identifikasi dan klasifikasi ikan.
Copyright (c) 2026 Raesya Cholida, Abdul Razak https://creativecommons.org/licenses/by/4.0
NARRATIVE REVIEW: POTENSI EKSTRAK BAWANG PUTIH (Allium sativum) DALAM MENINGKATKAN PERTUMBUHAN LACTOBACILLUS DAN MENGHAMBAT BAKTERI PENYEBAB DIARE
Diarrhea is a global health challenge exacerbated by increasing antibiotic resistance. This narrative review aims to analyze the potential of garlic extract (Allium sativum) as a natural synbiotic agent in supporting Lactobacillus growth while suppressing enteric pathogens. The method used was a narrative review with systematic literature searches through the Google Scholar and PubMed databases. The synthesis results show that garlic contains allicin and fructooligosaccharide (FOS) compounds at 3.34% w/w, which are effective in inhibiting pathogenic bacteria such as Escherichia coli and Salmonella spp., and are able to stimulate the growth of Lactobacillus acidophilus optimally at a concentration of 4%. In conclusion, Allium sativum has dual potential as an antibacterial agent and prebiotic, whose effectiveness is influenced by concentration and extraction method. Further research is needed to standardize the dosage for clinical application.
Copyright (c) 2026 Fadillah Amanda, Olyvia Azzahra Putri Hartono https://creativecommons.org/licenses/by/4.0
GENOME EDITING BERBASIS CRISPR-CAS9 UNTUK MENINGKATKAN TOLERANSI STRES ABIOTIK PADA TANAMAN PADI (ORYZA SATIVA): A SYSTEMATIC LITERATURE REVIEW
Abiotic stresses such as drought, salinity, extreme temperatures, and oxidative stress are major factors limiting the growth and productivity of rice plants. The development of CRISPR-Cas9-based genome editing technology provides a new, more precise approach to modifying genes that play a role in plants' adaptive responses to environmental stresses. This study aims to synthesize the development of genome editing research in rice plants, focusing on the identification of target genes, genome editing strategies, and their impact on increasing abiotic stress tolerance. The method used is a systematic literature review of research articles published in international scientific journals. A total of 31 articles were analyzed in the literature review process, and 22 articles that explicitly reported target genes and genome editing strategies were used in the main synthesis. The results of the analysis showed that most studies targeted regulatory genes that play a role in regulating stress responses, especially groups of transcription factors such as NAC, DREB, bZIP, MYB, and WRKY. In addition, several studies also targeted genes related to ion homeostasis, hormonal signaling pathways, secondary metabolism, and antioxidant systems. The most dominant genome editing strategy was gene knock-out through CRISPR-Cas9-based mutations, while several recent studies have begun to apply multiplex genome editing to target more than one gene simultaneously. Genome-edited rice plants generally show increased tolerance to various abiotic stresses through improved ion homeostasis, increased water use efficiency, cell membrane stability, and strengthened antioxidant systems. These findings indicate that CRISPR-Cas9 is a precision genome engineering approach with the potential to support the development of rice varieties that are more adaptive to environmental changes.
Copyright (c) 2026 Maya Putriwan, Sumarni, Rachmawaty https://creativecommons.org/licenses/by/4.0
EXPLORATION AND IDENTIFICATION OF MICROBES AND FUNGI FROM SOIL SAMPLES OF GUNGGUNG VILLAGE, BATUAN DISTRICT, SUMENEP REGENCY AS BIOLOGICAL CONTROL AGENTS AT THE EAST JAVA FOOD CROPS AND HORTICULTURE PLANT PROTECTION LABORATORY
Soil microorganisms play an essential role in maintaining soil fertility and suppressing plant pathogens, making them potential biological control agents in sustainable agriculture. This study aimed to explore and identify microbes and fungi from soil samples collected from yellow Kepok banana (Musa spp.) plantations in Gunggung Village, Batuan District, Sumenep Regency, as potential biological agents. The novelty of this research lies in the exploration of untreated Fusarium-infected banana plantation soil to obtain indigenous microbial isolates with biocontrol potential. A descriptive exploratory research design was employed. Bacterial isolation was conducted using the serial dilution method on Potato Dextrose Agar (PDA), followed by purification, macroscopic and microscopic characterization, 5% KOH test, soft rot test, and hypersensitivity test. Fungal isolation was performed using the baiting method with coconut, rice, and mealworms as substrates, followed by morphological identification. The results revealed the presence of Bacillus sp. as the dominant bacterial isolate, characterized by circular colonies, yellowish-white color, Gram-positive reaction, and rod-shaped cells. Soft rot and hypersensitivity assays indicated varying pathogenic responses among isolates. Fungal identification showed the presence of Aspergillus sp. (brownish-green, powdery colonies) and Penicillium sp. (yellowish-green, granular colonies). These findings indicate that soil from the study site contains microbial populations with potential applications as biological control agents. The study highlights the importance of exploring indigenous soil microorganisms to support environmentally friendly plant disease management and sustainable agricultural practices.
Copyright (c) 2026 Diva Anindita Darma, Wiwin Misnati Nur Indahsari https://creativecommons.org/licenses/by/4.0
VARIASI MORFOLOGI SISIK PADA IKAN FAMILI CYNOGLOSSIDAE: SEBUAH TINJAUAN LITERATUR
The tonguefish (family Cynoglossidae) is characterized by its flattened body and is widely distributed across the Indo-Pacific, India, Pakistan, and the Red Sea. This study aims to analyze the variation of scale types in the family Cynoglossidae based on a literature review. The literature search was conducted using electronic databases, namely Google Scholar, PubMed, and ScienceDirect. The keywords used in the search were "Cynoglossidae", "scale morphology", "tonguefish", and "scales". A total of 9 articles met the inclusion criteria and were extracted for data. Fifteen species of tonguefish were successfully identified in terms of their scale types. Three patterns of scale variation were found: ctenoid scales on both sides, ctenoid scales on the eyed side and cycloid scales on the blind side, and cycloid scales on both sides. The conclusion of this review is that the scale types in the family Cynoglossidae vary among species and show consistent patterns, thus they can serve as supporting characters in taxonomic identification and classification.
Copyright (c) 2026 Suchy Rahmadhani, Abdul Razak https://creativecommons.org/licenses/by/4.0
IDENTIFIKASI JENIS-JENIS KEPITING (BRACHYURA) YANG DIPERJUAL-BELIKAN DI PASAR KECAMATAN PADANG BARAT, KOTA PADANG
Crab trade in traditional markets is an important part of the fisheries distribution chain that reflects the utilization of marine resources by coastal communities. This study aims to identify the types of crabs (Brachyura) traded in traditional markets in Padang Barat District, Padang City, and to provide basic information for sustainable fisheries resource management. The research was conducted in February 2026 through direct observation at three traditional markets, namely Purus Fish Market, Raden Saleh Market, and Padang City Central Market. Data were collected through specimen documentation, species recording, and brief interviews with traders. The results of the study show that there are four species of crabs traded, all of which belong to the Portunidae family, namely Scylla serrata, Charybdis feriata, Portunus sanguinolentus, and Portunus pelagicus. The dominance of the Portunidae family indicates that swimming crabs are the main commodity in the marine product trade in the coastal area of West Sumatra. This study provides basic data on crab utilization in traditional markets and can support fisheries management, conservation of biological resources, and coastal economic development based on biodiversity.
Copyright (c) 2026 Rahmadana, Rijal Satria https://creativecommons.org/licenses/by/4.0
ANALISIS KERAGAMANGENETIK DNA UBI JALAR (Ipomea batatas L.)DENGAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)
Sweet potato (Ipomea batatas L.) is a national food security supported by one of the potential plant resources. After rice, corn, and cassava, sweet potato (Ipomea batatas L.) contains the fourth highest carbohydrate. Sweet potato is a good source of thiamine (vitamin B1), riboflavin, vitamin A, vitamin B, and vitamin C. The purpose of this study was to determine the genetics of the analysis results presented in sweet potato (Ipomea batatas L.) using dendograms and DNA banding patterns in sweet potatoes based on RAPD markers. The research method used is an experimental method to collect data by conducting direct experiments, isolating DNA, and conducting PCR using the RAPD technique; Six RAPD primers were used: OPA 2, OPA 3, OPA5, OPA7, OPD 11, and OPD 13. The study resulted in genetic diversity seen from the DNA banding pattern that forms polymorphism. RAPD produces bands measuring 100-900 bp. The genetic diversity of 4 types of Ipomea batatas L. plants analyzed using the NTSYS pc-2.02i application on the kinship relationship displayed with a dendogram shows the same diversity, namely orange sweet potatoes with yellow sweet potatoes, which have the highest level of similarity because they join at a coefficient value of around 0.91. While in purple sweet potatoes joining the group (yellow sweet potatoes and orange sweet potatoes) at a coefficient value of 0.86 indicates that purple sweet potatoes are slightly more different but still in the group. Then in white sweet potatoes have a connection coefficient value of around 0.81 which indicates that this sample is the most different compared to the others)
Copyright (c) 2026 Tri Wulan Dari, Zahratul Idami, Kartika Manalu https://creativecommons.org/licenses/by/4.0
ANALISIS FINGERPRINT DNA GENOTIPE BELIMBING DENGAN GENUS Averrhoa MENGGUNAKAN PRIMER RANDOM AMPLIFIED POLYMORPHIC
Fingerprinting is a technique in DNA technology that can be used to see individual diversity and can also differentiate individuals from each other even though they are very closely related. This study aims to see the results of DNA fingerprinting of starfruit genotypes with the genus Averrhoa using Random Amplified Polymorphic DNA (RAPD) primers and to see the polymorphism value of each Random Amplified Polymorphic DNA (RAPD) primer. The research method used is Fingerprint DNA with Random Amplified Polymorphic DNA (RAPD) primers, namely OPA-2, OPA-3, OPA-5, OPA-7, OPD-11 and OPD-13. The results of the research show that the fingerprint results can be seen clearly. using electrophoresis. The best results were the primers OPA 2, OPA3 with 6 band patterns, followed by the second highest number of OPA5, OPD 11 with 5 band patterns, and the third highest number of OPA7, OPD 13 with 4 DNA fingerprint band patterns, the results of the kinship relationship between each Averrhoa have The polymorphic value shows that sweet starfruit (Averrhoa carambola L.) has a relationship of around 0.62% with starfruit. wuluh ( Averrhoa bilimbi L.). The Demak starfruit (Averrhoa carambola Cultivar Demak) has a relationship of around 0.71% with the Sembiring starfruit (Averrhoa carambola).
Copyright (c) 2026 Nur Hikmah, Zahratul Idami, Kartika Manalu https://creativecommons.org/licenses/by/4.0
IDENTIFIKASI KERAGAMAN MORFOLOGI DAUN TANAMAN UBI KAYU (Manihot escolenta Cantz) BERDASARKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA)
Cassava Plants (Manihot esculenta Crantz), traditionally cultivated and offering many benefits as food ingredients, animal feed, and industrial raw materials. Cassava is also known to be rich in carbohydrates, soluble dietary fiber, and beneficial micronutrients for the body. Morphological and genetic diversity in cassava plants is crucial for the development of superior and improved varieties. This study aims to identify the morphological diversity of cassava leaf plants (Manihot esculenta Crantz) and analyze dendrogram relationships using RAPD (Random Amplified Polymorphic DNA) markers. The research was conducted in Serdang Bedagai Regency and the laboratory of the State Islamic University of North Sumatra, Medan. The methods used included sample collection, DNA isolation, PCR amplification, electrophoresis, and data analysis. The results showed variations in leaf morphology as observed in the number of leaflets, color, and shape of leaves in cassava varieties Genderuwo, Roti, Keriting, and Jari. Morphologically, the Genderuwo and Roti cassava leaves were the most similar. The dendrogram analysis using RAPD primers OPA 2, OPA 3, OPA 5, and OPA 7 showed a close relationship between Genderuwo and Roti varieties (coefficient 0.85), while the Jari variety had the most distant relationship (coefficient 0.60). This research can serve as a reference in cassava plant breeding to produce superior varieties
Copyright (c) 2026 Hafiza Hazmi, Zahratul Idami, Kartika Manalu https://creativecommons.org/licenses/by/4.0
PENGARUH VIDEO PEMBELAJARAN BIOLOGI TERHADAP MOTIVASI DAN HASIL BELAJAR SISWA PADA MATERI SISTEM PEREDARAN DARAH KELAS XI MAS. AL-IHSAN PANDEGLANG
Biology learning often involves abstract concepts, such as the circulatory system, which students find difficult to understand through conventional methods. This study employed a quasi-experimental design with a quantitative approach. A sample of 42 eleventh-grade students from MAS Al-Ihsan Pandeglang was divided into an experimental group, which used video media, and a control group, which used conventional methods. Data were collected through motivation questionnaires and multiple-choice tests. The results showed that the experimental group achieved a significantly higher average N-Gain score of 56.50%, compared to 21.25% for the control group. Furthermore, student motivation was high, with 93.21% of students exhibiting a positive attitude toward the use of learning videos. In conclusion, the use of biology learning videos significantly enhances both student motivation and learning outcomes regarding the circulatory system materiallearning outcomes and motivation compared to conventional methods.
Copyright (c) 2026 Yeni Haerani, Hermawan, Rina Nuraisyah https://creativecommons.org/licenses/by/4.0
EKSPLORASI SERTA IDENTIFIKASI BAKTERI DAN CENDAWAN TANAH KABUPATEN SAMPANG SEBAGAI AGENS HAYATI DI LABORATORIUM AGENS HAYATI UPT PTPH JAWA TIMUR
Exploration of soil microorganisms as biological control agents is one of the environmentally friendly approaches to controlling plant pests and diseases. This study aimed to explore and identify soil bacteria and fungi with potential as biological control agents at the Biological Control Laboratory of the Technical Implementation Unit for Food Crops and Horticultural Plant Protection, East Java. The research was conducted from January to April 2025 through bacterial isolation using the serial dilution method and fungal isolation using the baiting method, followed by macroscopic and microscopic observations as well as physiological and biochemical tests. The results showed that two soil bacterial isolates were obtained, in which one isolate was suspected to be Bacillus sp. with potential as a biological control agent, while the other isolate showed no potential as a biological control agent. A bacterial isolate initially suspected as Bacillus thuringiensis was not identified as an entomopathogenic bacterium based on physiological and biochemical tests. Fungal exploration resulted in isolates suspected as Trichoderma sp. and Aspergillus sp
Copyright (c) 2026 Dyah Putri Yudha Yanti, Wiwin Misnati Nur Indahsari https://creativecommons.org/licenses/by/4.0